Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring

Khosravizadeh, Zahra; Khodamoradi, Kajal; Rashidi, Zahra; Jahromi, Malihe; Shiri, Elham; Salehi, Ensieh; Talebi, Ali

doi:10.1007/s10815-022-02545-6

Cited by 18 publications

(12 citation statements)

References 81 publications

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“…Given the much clearer and greater increased risks to female fertility with increasing age, such considerations may well be magnified in relation to sperm cryopreservation for men, particularly given the more limited intervention required to obtain cryostorable gametes. However, cryopreservation of sperm has adverse effects on sperm quality and function [ 142 ] and thus a greater requirement for later use of assisted conception treatments. These implications are likely to outweigh the risk of increasing paternal age.…”

Section: Addressing the Issues Of Advanced Paternal Agementioning

confidence: 99%

Reproductive axis ageing and fertility in men

Silva

Anderson

2022

Rev Endocr Metab Disord

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Compared to women, increasing male age is not accompanied by such marked changes in reproductive function but changes certainly do happen. These include alterations to the hypothalamo-pituitary-testicular axis, with resultant implications for testosterone production and bioavailability as well as spermatogenesis. There is a decline in sexual function as men age, with a dramatic increase in the prevalence of erectile dysfunction after the age of 40, which is a marker for both clinically evident as well as covert coronary artery disease. Despite a quantitative decline in spermatogenesis and reduced fecundability, the male potential for fertility persists throughout adult life, however there are also increasingly recognised alterations in sperm quality and function with significant implications for offspring health. These changes are relevant to both natural and medically assisted conception.

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Section: Addressing the Issues Of Advanced Paternal Agementioning

confidence: 99%

Reproductive axis ageing and fertility in men

Silva

Anderson

2022

Rev Endocr Metab Disord

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“…Oxidative stress refers to a state in which there is an imbalance between reactive oxygen species (ROS) and antioxidants in the body. While a small amount of ROS is necessary for normal physiological processes in the body, such as sperm capacitation and the acrosome reaction ( 6 ), an excess of ROS can cause damage to the body, including DNA damage, changes in protein structure, abnormal hormone levels, lipid peroxidation (LPO), and even epigenetic changes ( 7 ). It is now believed that the sources of ROS in seminal plasma mainly include immature sperm and white blood cells.…”

Section: Introductionmentioning

confidence: 99%

Analysis of research trends (2014-2023) on oxidative stress and male fertility based on bibliometrics and knowledge graphs

Du,

Yu,

Fan

2024

Front. Endocrinol.

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BackgroundOxidative stress (OS) is considered one of the major factors affecting male fertility, and research in this field has seen constant growth year by year. Currently, around 700 relevant papers are published each year, with a trend of further growth. Therefore, this study systematically summarizes the literature published in the last decade from a bibliometric perspective, revealing the dynamic development of the field, identifying research hotspots, analyzing future trends, and providing reference for further research.MethodsRelevant literature on oxidative stress and male fertility was retrieved from the Web of Science Core Collection (WoSCC) database, covering the timespan from 2014 to 2023 and including two types, articles and reviews. CiteSpace and VOSviewer were used for bibliometric analysis, including cluster analysis, co-occurrence analysis, co-citation analysis, and burst analysis of countries/regions, institutions, journals, authors, references, and keywords.ResultsThis paper studied a total of 5,301 papers involving 107 countries/regions, with China having the highest number of publications (898 papers) and the United States having the highest centrality (0.62). Burst analysis of journal citations revealed the emergence of many new journals (e.g., Antioxidants-Basel, Front Endocrinol) after 2021, indicating continuous expansion and development in this field. Cluster analysis of co-cited references and co-occurring keywords divided the research into areas such as oxidative stress and male infertility, oxidative stress level detection, and antioxidants. The keywords associated with research hotspots shifted from oxidative stress detection, sperm DNA damage, apoptosis, and redox potential to DNA methylation, embryonic development, infection, polyunsaturated fatty acids, and antioxidants.ConclusionBibliometric methods provide an intuitive reflection of the development process in the field of oxidative stress and male fertility, as well as the analysis of research hotspots in different periods. Research on oxidative stress and embryonic development, as well as antioxidant health management, may become hotspots in future research.

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“…However, the cold shock and alterations in atmospheric oxygen seriously led to biochemical, biophysical, ultrastructural, molecular, and functional alterations ( 5 – 7 ). These alterations in turn trigger excessive production and accumulation of reactive oxygen species (ROS) in mammalian semen ( 8 – 11 ). The imbalance between free radicals and the antioxidant defense system in semen caused by altered ROS production levels led to oxidative stress and osmotic stressors with subsequent acrosomal damage, mitochondrial membrane depolarization, and disruption of plasma membrane permeability ( 12 – 16 ).…”

Section: Introductionmentioning

confidence: 99%

Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

Ren

Zhang

et al. 2023

Front. Vet. Sci.

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IntroductionIn the present study, the synergistic protective effect of co-supplementation of glutathione (GSH) with selenium nanoparticles (SeNPs) on the cryopreservation efficiency of bull semen was analyzed.MethodsAfter collection, the ejaculates of Holstein bulls were subsequently diluted with a Tris extender buffer supplemented with different concentrations of SeNPs (0, 1, 2, and 4 μg/ml), followed by semen equilibration at 4°C and assessment of sperm viability and motility. Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 μg/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 μg/ml SeNPs (GSH + SeNPs group). After cryopreservation, motility, viability, mitochondrial activity, plasma membrane integrity, acrosome integrity, concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), and ability of frozen-thawed sperm cells to support in vitro embryonic development were evaluated.Results and discussionNo side effect of SeNPs concentrations applied in the current study on the motility and viability of equilibrated bull spermatozoa was found. Meanwhile, supplementation of SeNPs significantly promoted the motility and viability of equilibrated bull spermatozoa. Furthermore, the co-supplementation of GSH with SeNPs effectively protected bull spermatozoa from cryoinjury as expressed by promoting semen motility, viability, mitochondrial activity, plasma membrane integrity, and acrosome integrity. Finally, the enhanced antioxidant capacity and embryonic development potential in the frozen-thawed bull spermatozoa cryopreserved by co-supplementation of GSH with SeNPs further confirmed the synergistic protective effect of co-supplementation of GSH with SeNPs on the cryopreservation of bull semen.

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Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring

Cited by 18 publications

References 81 publications

Reproductive axis ageing and fertility in men

Reproductive axis ageing and fertility in men

Analysis of research trends (2014-2023) on oxidative stress and male fertility based on bibliometrics and knowledge graphs

Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

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