Sperm Cryopreservation

Todorovic, Biljana Popovic; Verheyen, Greta; Vloeberghs, Veerle; Tournaye, Herman

doi:10.1007/978-3-030-47767-7_36

Cited by 2 publications

(2 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rapid freezing method is based on the direct contact of samples with liquid nitrogen vapor for at least 10 minutes, in this method the sperm are mixed with cryoprotective agent and placed in cryotubes that will be exposed to nitrogen vapors. After the vapor exposure phase; the samples are immersed in liquid nitrogen ( 140 , 141 ). The addition of CPAs to the samples seeks to minimize osmotic damage and prevent intracellular and extracellular ice crystals from forming, however, CPAs have cytotoxic characteristics ( 142 ).…”

Section: Current Strategies To Reestablish Testicular Functionalitymentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

View full text Add to dashboard Cite

Traditional therapeutic interventions aim to restore male fertile potential or preserve sperm viability in severe cases, such as semen cryopreservation, testicular tissue, germ cell transplantation and testicular graft. However, these techniques demonstrate several methodological, clinical, and biological limitations, that impact in their results. In this scenario, reproductive medicine has sought biotechnological alternatives applied for infertility treatment, or to improve gamete preservation and thus increase reproductive rates in vitro and in vivo. One of the main approaches employed is the biomimetic testicular tissue reconstruction, which uses tissue-engineering principles and methodologies. This strategy pursues to mimic the testicular microenvironment, simulating physiological conditions. Such approach allows male gametes maintenance in culture or produce viable grafts that can be transplanted and restore reproductive functions. In this context, the application of several biomaterials have been proposed to be used in artificial biological systems. From synthetic polymers to decellularized matrixes, each biomaterial has advantages and disadvantages regarding its application in cell culture and tissue reconstruction. Therefore, the present review aims to list the progress that has been made and the continued challenges facing testicular regenerative medicine and the preservation of male reproductive capacity, based on the development of tissue bioengineering approaches for testicular tissue microenvironment reconstruction.

show abstract

Section: Current Strategies To Reestablish Testicular Functionalitymentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Thus, it is more urgent to search for more affordable methods of assessing the quality of spermatozoa, including the analysis of the morphological specifi cities of their structure, which would be used as an instrument for cryopreservation effi ciency screening. The methods of spermatozoa cryopreservation are known to be suboptimal (Todorovic et al, 2022). It was proven regarding many species of animals that the spermatozoa survival rate after thawing depended on their initial morphofunctional characteristics (Dorado et al, 2010;Casas et al, 2009;Núñez-Martínez et al, 2007;Pavlovych et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Morphological and morphometric characteristics of fresh and cryopreserved spermatozoa of saanen bucks

Bogdaniuk

Petrushko

2023

Agric. sci. pract.

View full text Add to dashboard Cite

Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout the year to effectively increase livestock. Aim. To evaluate the influence of seasons on morphological and morphometric characteristics of fresh and cryopreserved goat sperm. Methods. The ejaculate samples of three sexually mature bucks of the Saanen breed were obtained during the breeding and non-breeding seasons. To assess the morphological characteristics, the smears of sperm were stained and then visualized under a light microscope with a magnification of ×1,000. For morphometric measurements, sperm micrographs were taken and analyzed using the ImageJ software. The cryopreservation of sperm was performed in a medium with 10 % glycerol and 20 % egg yolk. The samples were thawed in a water bath, then the morphological and morphometric characteristics of the cells were evaluated. Results. After the cryopreservation, the number of sperm with abnormal morphological structures was significantly higher compared to fresh sperm, both in the breeding and non-breeding seasons (p ≤ 0.05). After the cryopreservation, the length and width of the head decreased significantly (p ≤ 0.05) compared to the corresponding sperm sizes before the cryopreservation. Analyzing the morphological char- acteristics of the tail part, we revealed the fact of its reduction after the cryopreservation, probably due to twisting, loop formation, and detachment of its part. Conclusions. It was found that in the non-breeding season, the number of spermatozoa with damaged morphological structures increases, compared to the breeding season, which must be taken into account when using reproductive technologies. The morphological and morphometric characteristics of Saanen buck spermatozoa change after the cryopreservation, regardless of the season, which indicates the need to choose the fertilization tactics that will ensure the selection of spermatozoa without any morphological abnormalities.

show abstract

Sperm Cryopreservation

Cited by 2 publications

References 111 publications

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

Morphological and morphometric characteristics of fresh and cryopreserved spermatozoa of saanen bucks

Contact Info

Product

Resources

About