Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART

Bungum, Mona; Spanò, Marcello; Humaıdan, Peter; Eleuteri, Patrizia; Rescia, Michele; Giwercman, Aleksander

doi:10.1093/humrep/dem353

Cited by 102 publications

(90 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The negative effects of apoptosis on fertilization and pregnancy are less present or even absent. Another explanation for the absence of predictive power of DNA damage for IVF/ICSI outcomes might be that when using DGC, which reduces the percentage of sperm with DNA denaturation, there exist other types of DNA damage that are not detected by acridine orange [8], but additionally affecting IVF/ICSI outcomes. As a consequence, sperm DNA integrity may or may not have an impact on the clinical results of IVF and ICSI cycles [12].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate the relationship between sperm apoptotic biomarkers and patient clinical characteristics, conventional sperm parameters and fertility potential. Material and methods Sperm analysis, phospholipid asymmetry, mitochondrial membrane potential (MMP) and DNA denaturation were assessed in 142 males of infertile couples. Seventy-three couples were allocated to the natural conception group, and 55 couples underwent IVF or ICSI. Results DNA denaturation correlated positively with age and negatively with testicular volume (TV). MMP correlated negatively with BMI and FSH and positively with TV. Normal viable sperm correlated positively with TV and negatively with age, BMI and FSH. DNA denaturation was associated with a significantly lower natural pregnancy rate (OR 5.4, p00.011). Conclusion Sperm apoptosis is related to male age, BMI, testicular volume and FSH. Among the apoptotic markers, only DNA denaturation has been found to predict natural pregnancy better than conventional sperm parameters.

show abstract

Section: Discussionmentioning

confidence: 99%

“…According to some authors, it might provide additional information regarding the outcome of in vitro fertilization [6,14,26,46]. However, there are authors denying this property to DNA damage [8,12,48].…”

Section: Introductionmentioning

confidence: 99%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…This is very important if a DNA integrity test will be included in the panel as DNA integrity testing has recently been challenged as not having clinical relevance when used to evaluate male infertility [59]. The need to reevaluate old test scores is best illustrated by how a reevaluation of the use of the SCSA DNA fragmentation index (DFI) scores has dramatically changed how the test results are used to direct patients to appropriate ART [16,17]. The SCSA is the only DNA integrity test with substantial scientific literature supporting the credibility of this test for use in diagnosing male infertility, and up until 2007, patients whose sperm had a DNA fragmentation index (DFI)≥30 were told that their sperm had minimal to no chance for successful ART attempts at pregnancy [22,31,32].…”

Section: Discussionmentioning

confidence: 99%

“…The reason for what many interpreted to be a loss of the SCSA's utility to predict ART outcome was not understood until the SCSA was evaluated for utility in predicting live birth outcome in each of the commonly used ART treatments, analyzing IUI, IVF, and ICSI live birth outcome as separate data sets. When this was done, it was found that the DFI significantly predicted live birth outcome in IUI cycles, but had no utility in predicting IVF or ICSI live birth outcome [16]. At present, the suggested course of treatment for patients with abnormal SCSA scores is that they be directed to ICSI, as this procedure has a higher live birth outcome rate than IVF.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Brown

Merryman²,

Rivnay

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD TM ) Test, 2) Sperm DNA Decondensation (SDD TM ) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully. Methods A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple). Results The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate040 % (n060 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p00.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle. Conclusion SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

show abstract

Sperm Chromatin and ART (IUI, IVF and ICSI) Pregnancy

Bungum

2011

Sperm Chromatin

View full text Add to dashboard Cite

Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART

Abstract: SCSA performed in semen prepared by DGC cannot predict the outcome of ART.

Cited by 102 publications

References 50 publications

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Sperm Chromatin and ART (IUI, IVF and ICSI) Pregnancy

Contact Info

Product

Resources

About