Sperm characterization of the endangered Amazonian fish Hypancistrus zebra: Basic knowledge for reproduction and conservation strategies

Caldas, Jôsie Schwartz; Godoy, Leandro

doi:10.1016/j.anireprosci.2019.03.012

Cited by 16 publications

(11 citation statements)

References 18 publications

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“…Three samples of sperm (10 μL) from healthy (n = 5) and bleached (n = 8) colonies were placed on histological glass slides and the motility rate was assessed under a microscope (40× magnification). The percentage of motile cells exhibiting forward movement was recorded at 0, 3 and 4 h after spawning to determine spermatozoa lifespan, i.e., the full arrest of motility for all spermatozoa in the optical field (Caldas and Godoy 2019). To evaluate sperm concentration per bundle, 20 μL of sperm from healthy (n = 4) and bleached (n = 3) colonies (10 bundles colony −1 ) was fixed in 40 μL of 4% buffered formal saline solution (Hancoch 1957).…”

Section: Gamete Concentration and Quality Assessmentsmentioning

confidence: 99%

Southwestern Atlantic reef-building corals Mussismilia spp. are able to spawn while fully bleached

et al. 2021

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Bleaching events are becoming more frequent and intense worldwide. Southwestern Atlantic corals are considered stresstolerant species that suffer less during bleaching episodes. Therefore, we investigated if the reef-building corals Mussismilia hispida and M. harttii are capable of spawning while fully bleached. We compared spermatozoa concentration and motility, egg diameter and embryo viability between seemingly healthy and bleached colonies for both species. Findings show that both species spawned viable gametes. Concentration and motility of newly-released spermatozoa were similar between healthy and bleached colonies for both species. Unlike M. hispida, a 10% reduction in size was found in egg diameter for M. harttii. Embryo viability for bleached M. hispida colonies did not decrease; for bleached M. harttii there was a reduction from 14.7 to 6.3%, but fertilization and larval development still took place. These findings describe the unprecedented episode of corals spawning viable gametes when entirely bleached, supporting the suggestion of greater resilience for Southwestern Atlantic corals.

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Section: Gamete Concentration and Quality Assessmentsmentioning

confidence: 99%

Southwestern Atlantic reef-building corals Mussismilia spp. are able to spawn while fully bleached

et al. 2021

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“…The typical duration of motility is short, ranging from one to 2 min (Dreanno et al, 1998;Poleo et al, 2001). This short duration, also observed in M. oligolepis, is related to rapid energy expenditure after the onset of motility (Caldas and Godoy, 2019). This also manifests in decreased sperm vigour with time (Murgas et al, 2011).…”

Section: Discussionmentioning

confidence: 97%

“…This requires an understanding of both male and female gametes to succeed. Although there has been a growing body of research on male gametes, as exemplified by Dreanno et al (1998), Streit Jr et al (2002; Paulino et al (2012), Silva et al (2014), Lenz et al (2018), Caldas and Godoy (2019) and Kowalski and Cejko (2019), very little information is known about the semen of many native species, including the fire-eye tetra, M. oligolepis.…”

Section: Introductionmentioning

confidence: 99%

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Rodrigues

Santos

Cordeiro

et al. 2020

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Summary The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

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“…Em machos a qualidade espermática pode ser avaliada a partir da análise de parâmetros espermáticos pós-coleta e submetidos à ativação por diferentes ativadores 86 , já que os espermatozoides são imóveis no testículo e só entram em movimento após o contato com uma solução geralmente hipo-osmótica ao plasma seminal 87 . Porém, antes da ativação espermática a qualidade pode ser estimada com a avaliação dos parâmetros de integridade de membrana 88 integridade de DNA 89 , metilação de DNA 90 , peroxidação lipídica 91 , estresse oxidativo 92 e índices de alterações morfológicas 93 . Além disso, determinação da concentração espermática 94 , pH e composição do plasma seminal 95 também podem ser avaliados.…”

Section: Avaliação Da Qualidade De Gametasunclassified

Aspectos Econômico Da Piscicultura Na Amazônia Brasileira

Costa¹

2021

Aquicultura Na Amazônia: Estudos Técnico-Científicos E Difusão De Tecnologias

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica. A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.

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Sperm characterization of the endangered Amazonian fish Hypancistrus zebra: Basic knowledge for reproduction and conservation strategies

Cited by 16 publications

References 18 publications

Southwestern Atlantic reef-building corals Mussismilia spp. are able to spawn while fully bleached

Southwestern Atlantic reef-building corals Mussismilia spp. are able to spawn while fully bleached

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Aspectos Econômico Da Piscicultura Na Amazônia Brasileira

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