Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility – a pilot study

Jaiswal, Ankit; Baliu-Souza, Tatiana; Turner, Katerina; Nadiminty, Nagalakshmi; Rambhatla, Amarnath; Agarwal, Ashok; Krawetz, Stephen A.; Dupree, James M.; Saltzman, Barbara; Schon, Samantha B.; Avidor‐Reiss, Tomer

doi:10.1016/j.ejcb.2022.151243

Cited by 10 publications

(9 citation statements)

References 85 publications

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“…This technique found that patients diagnosed with teratozoospermia (poor morphology) had lower centriole quality (Turner et al, 2021), as was suggested earlier by electron microscopy studies (Alosilla Nakamura et al, 2002;Porcu, 2003;Rawe et al, 2002;Sha et al, 2017;Terada et al, 2009). Also, FRAC identi es male factor infertility in couples with unexplained infertility (Jaiswal et al, 2022). FRAC is a sensitive method for identifying male infertility because it detects changes in relative protein distribution using uorescent microscopy.…”

Section: Introductionmentioning

confidence: 77%

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Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study

Turner

Achinger

Kong

et al. 2023

Preprint

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The mechanisms underlying male infertility are poorly understood. Most mammalian spermatozoa have two centrioles: the typical barrel-shaped proximal centriole (PC) and the atypical fan-like distal centriole (DC) connected to the axoneme (Ax). These structures are essential for fertility. However, the relationship between centriole quality and subfertility (reduced fertility) is not well established. Here, we tested the hypothesis that assessing sperm centriole quality can identify cattle subfertility. By comparing sperm from 25 fertile and 6 subfertile bulls, all with normal semen analyses, we found that unexplained subfertility and lower sire conception rates (pregnancy rate from artificial insemination in cattle) corelate with abnormal centriolar biomarker distribution. Fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) found only four fertile bulls (4/25, 16%) had positive FRAC tests (having one or more mean FRAC ratios outside of the distribution range in a group’s high-quality sperm population), whereas all of the subfertile bulls (6/6, 100%) had positive FRAC tests (P=0.00008). The most sensitive biomarker was Acetylated Tubulin, which had a novel labeling pattern between the DC and Ax. These data suggest that FRAC and Acetylated Tubulin labeling can identify bull subfertility that remains undetected by current methods and may provide insight into a novel mechanism of subfertility.

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Section: Introductionmentioning

confidence: 77%

“…Centriolar biomarkers can be structural proteins or posttranslational modi cations (Jaiswal et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study

Turner

Achinger

Kong

et al. 2023

Preprint

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“…Of the remaining 375 papers, 117 did not classify a definition of unexplained infertility. As a result, a total of 258 primary research papers with full text available were included …”

Section: Resultsmentioning

confidence: 99%

The definition of unexplained infertility: A systematic review

Raperport,

Desai,

Qureshi

et al. 2023

BJOG

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BackgroundThere is no consensus on tests required to either diagnose unexplained infertility or use for research inclusion criteria. This leads to heterogeneity and bias affecting meta‐analysis and best practice advice.ObjectivesThis systematic review analyses the variability of inclusion criteria applied to couples with unexplained infertility. We propose standardised criteria for use both in future research studies and clinical diagnosis.Search strategyCINAHL and MEDLINE online databases were searched up to November 2022 for all published studies recruiting couples with unexplained infertility, available in full text in the English language.Data collection and analysisData were collected in an Excel spreadsheet. Results were analysed per category and methodology or reference range.Main resultsOf 375 relevant studies, only 258 defined their inclusion criteria. The most commonly applied inclusion criteria were semen analysis, tubal patency and assessment of ovulation in 220 (85%), 232 (90%), 205 (79.5%) respectively. Only 87/220 (39.5%) studies reporting semen analysis used the World Health Organization (WHO) limits. Tubal patency was accepted if bilateral in 145/232 (62.5%) and if unilateral in 24/232 (10.3%). Ovulation was assessed using mid‐luteal serum progesterone in 115/205 (56.1%) and by a history of regular cycles in 87/205 (42.4%). Other criteria, including uterine cavity assessment and hormone profile, were applied in less than 50% of included studies.ConclusionsThis review highlights the heterogeneity among studied populations with unexplained infertility. Development and application of internationally accepted criteria will improve the quality of research and future clinical care.

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“…However, in recent years, many countries have witnessed a significant decline in birth rates, leading to an acceleration in population aging. Infertility affecting approximately 12%–20% of couples is a major factor in the declining birth rate (Jaiswal et al., 2022). The incidence of infertility is on the rise.…”

Section: Neu5gcmentioning

confidence: 99%

“…Infertility affecting approximately 12%-20% of couples is a major factor in the declining birth rate (Jaiswal et al, 2022). The incidence of infertility is on the rise.…”

Section: Neu5gc and Risk Of Infertilitymentioning

confidence: 99%

N‐glycolylneuraminic acid in red meat and processed meat is a health concern: A review on the formation, health risk, and reduction

Liang,

Wu,

et al. 2024

Comp Rev Food Sci Food Safe

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One of the most consistent epidemiological associations between diet and human disease risk is the impact of consuming red meat and processed meat products. In recent years, the health concerns surrounding red meat and processed meat have gained worldwide attention. The fact that humans have lost the ability to synthesize N‐glycolylneuraminic acid (Neu5Gc) makes red meat and processed meat products the most important source of exogenous Neu5Gc for humans. As our research of Neu5Gc has increased, it has been discovered that Neu5Gc in red meat and processed meat is a key factor in many major diseases. Given the objective evidence of the harmful risk caused by Neu5Gc in red meat and processed meat to human health, there is a need for heightened attention in the field of food. This updated review has several Neu5Gc aspects given including biosynthetic pathway of Neu5Gc and its accumulation in the human body, the distribution of Neu5Gc in food, the methods for detecting Neu5Gc, and most importantly, a systematic review of the existing methods for reducing the content of Neu5Gc in red meat and processed meat. It also provides some insights into the current status and future directions in this area.

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Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility – a pilot study

Cited by 10 publications

References 85 publications

Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study

Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study

The definition of unexplained infertility: A systematic review

N‐glycolylneuraminic acid in red meat and processed meat is a health concern: A review on the formation, health risk, and reduction

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