Sperm cell heterogeneity revealed by centrifugal counter-current distribution in an aqueous two-phase system

Quality characteristics and fertilizing ability of ram sperm subpopulations separated by partition in an aqueous two-phase system

“…We have shown that CCCD in an aqueous TPS is a resolute technique revealing sperm heterogeneity [22][23][24], the functional variability in response of spermatozoa, which is known to be an inherent characteristic of sperm cells highly involved in successful fertilization [25,26]. This heterogeneity appears to be associated with different membranes integrity states of the fractionated cells in the CCCD process [24,27], and also with different maturation states [25].…”

“…This heterogeneity appears to be associated with different membranes integrity states of the fractionated cells in the CCCD process [24,27], and also with different maturation states [25]. Moreover, CCCD has been revealed as a useful tool for the estimation of the fertilizing potential of a given semen sample, taking into account the heterogeneity of the profile together with the viability values in the central chambers [28].…”

Centrifugal countercurrent chromatography to elucidate surface differences of adipose tissue‐derived stem cells

“…In order to design a suitable TPS for the study of ASC heterogeneity by CCCD, we tested a system composed of dextran, PEG, and Ficoll. Our previous works on sperm cell separation have shown that the inclusion of either Percoll or Ficoll can be useful to avoid cell sedimentation when centrifugation is used to shorten the phase separation process []. This issue is of particular interest for the separation of cells by phase partitioning, because the important shortening of the separation process promoted by enhancement of the phase settling can prevent cell death.…”

Management Factors Affecting Fertility in Sheep

“…Long abstinence periods (Pascual, 1993) and successive ejaculations (Ollero et al, 1994) have been associated with membrane alterations of spermatozoa. A decrease in semen volume and sperm concentration with successive ejaculations has been reported in several studies (Ollero et al, 1996;Kaya et al, 2002).…”