Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca<sup>2+</sup> Imaging

Hartmann, Constanze; Triller, Annika; Spehr, Marc; Dittrich, Ralf; Hatt, Hanns; Buettner, Andrea

doi:10.1002/cplu.201300008

Cited by 19 publications

(25 citation statements)

References 64 publications

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“…This receptor was functionally characterized in human testis and spermatozoa [64,65]. OR7C1 showed reliable expression in all TG samples investigated (FPKM values up to 1.7) but not in the DRG sample.…”

Section: Resultsmentioning

confidence: 99%

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

Flegel

Schöbel

Altmüller³

et al. 2015

PLoS ONE

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168

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The chemosensory capacity of the somatosensory system relies on the appropriate expression of chemoreceptors, which detect chemical stimuli and transduce sensory information into cellular signals. Knowledge of the complete repertoire of the chemoreceptors expressed in human sensory ganglia is lacking. This study employed the next-generation sequencing technique (RNA-Seq) to conduct the first expression analysis of human trigeminal ganglia (TG) and dorsal root ganglia (DRG). We analyzed the data with a focus on G-protein coupled receptors (GPCRs) and ion channels, which are (potentially) involved in chemosensation by somatosensory neurons in the human TG and DRG. For years, transient receptor potential (TRP) channels have been considered the main group of receptors for chemosensation in the trigeminal system. Interestingly, we could show that sensory ganglia also express a panel of different olfactory receptors (ORs) with putative chemosensory function. To characterize OR expression in more detail, we performed microarray, semi-quantitative RT-PCR experiments, and immunohistochemical staining. Additionally, we analyzed the expression data to identify further known or putative classes of chemoreceptors in the human TG and DRG. Our results give an overview of the major classes of chemoreceptors expressed in the human TG and DRG and provide the basis for a broader understanding of the reception of chemical cues.

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Section: Resultsmentioning

confidence: 99%

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

Flegel

Schöbel

Altmüller³

et al. 2015

PLoS ONE

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156

168

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“…OR1D2 is localized to the midpiece of human spermatozoa (Neuhaus et al, 2006 ). OR4D1 and OR7A5 are expressed in human testis and sperm and they can be activated by naturally occurring ligands (OR4D1: 5a-androst-16-en-3-one; OR7A5: 4-hydroxy-2,5-dimethyl-3(2H)-furanone) (Hartmann et al, 2013 ). For all three ORs, their activations were linked to a chemokinetic function in human spermatozoa (Veitinger et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…We were able to deorphanize three newly identified spermatozoal ORs (OR2W3-nerol; OR10J1-dimetol; OR2H1-methional). Interestingly, methional is an endogenously occurring ligand in the female reproductive tract (Hartmann et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, we showed that OR1D2 (OR17-4) can be activated by the synthetic odorant bourgeonal (Spehr et al, 2003 ) and is localized in the midpiece of human spermatozoa (Neuhaus et al, 2006 ). In addition, OR4D1 and OR7A5 are expressed in human testis, and sperm can be activated by the synthetic as well as the naturally occurring ligands of these two receptors (Veitinger et al, 2011 ; Hartmann et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Olfactory Receptors Expressed in Human Spermatozoa

Flegel

Vogel

Hofreuter

et al. 2016

Front. Mol. Biosci.

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100

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The detection of external cues is fundamental for human spermatozoa to locate the oocyte in the female reproductive tract. This task requires a specific chemoreceptor repertoire that is expressed on the surface of human spermatozoa, which is not fully identified to date. Olfactory receptors (ORs) are candidate molecules and have been attributed to be involved in sperm chemotaxis and chemokinesis, indicating an important role in mammalian spermatozoa. An increasing importance has been suggested for spermatozoal RNA, which led us to investigate the expression of all 387 OR genes. This study provides the first comprehensive analysis of OR transcripts in human spermatozoa of several individuals by RNA-Seq. We detected 91 different transcripts in the spermatozoa samples that could be aligned to annotated OR genes. Using stranded mRNA-Seq, we detected a class of these putative OR transcripts in an antisense orientation, indicating a different function, rather than coding for a functional OR protein. Nevertheless, we were able to detect OR proteins in various compartments of human spermatozoa, indicating distinct functions in human sperm. A panel of various OR ligands induced Ca2+ signals in human spermatozoa, which could be inhibited by mibefradil. This study indicates that a variety of ORs are expressed at the mRNA and protein level in human spermatozoa.

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“…Few studies have explored the pathways used to identify bioactive substances that elicit responses from conspecific insect sperm, and it is unlikely that the volatile AgOr ligands used here comprise the endogenous signals involved in A. gambiae spermatozoa activation. Indeed, endogenous ligands for human sperm ORs have recently been characterized, which are distinct from their previously identified volatile ligands (39). Examples of directed movement of sperm have been extensively characterized in marine invertebrates and mammals (reviewed in ref.…”

Section: Significancementioning

confidence: 99%

Odorant receptor-mediated sperm activation in disease vector mosquitoes

Pitts

Liu

Zhou

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Insects, such as the malaria vector mosquito, Anopheles gambiae, depend upon chemoreceptors to respond to volatiles emitted from a range of environmental sources, most notably blood meal hosts and oviposition sites. A subset of peripheral signaling pathways involved in these insect chemosensory-dependent behaviors requires the activity of heteromeric odorant receptor (OR) ion channel complexes and ligands for numerous A. gambiae ORs (AgOrs) have been identified. Although AgOrs are expressed in nonhead appendages, studies characterizing potential AgOr function in nonolfactory tissues have not been conducted. In the present study, we explore the possibility that AgOrs mediate responses of spermatozoa to endogenous signaling molecules in A. gambiae. In addition to finding AgOr transcript expression in testes, we show that the OR coreceptor, AgOrco, is localized to the flagella of A. gambiae spermatozoa where Orco-specific agonists, antagonists, and other odorant ligands robustly activate flagella beating in an Orco-dependent process. We also demonstrate Orco expression and Orco-mediated activation of spermatozoa in the yellow fever mosquito, Aedes aegypti. Moreover, we find Orco localization in testes across distinct insect taxa and posit that OR-mediated responses in spermatozoa may represent a general characteristic of insect reproduction and an example of convergent evolution.transcriptomics | cell signaling | gamete | chemical ecology | Culicidae

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Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca²⁺ Imaging

Abstract: Reagents for cell culture use were purchased from Invitrogen, unless stated otherwise. Human embryonic kidney 293 (HEK293)

Cited by 19 publications

References 64 publications

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

Characterization of the Olfactory Receptors Expressed in Human Spermatozoa

Odorant receptor-mediated sperm activation in disease vector mosquitoes

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Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca2+ Imaging

Abstract: Reagents for cell culture use were purchased from Invitrogen, unless stated otherwise. Human embryonic kidney 293 (HEK293)

Cited by 19 publications

References 64 publications

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

Characterization of the Olfactory Receptors Expressed in Human Spermatozoa

Odorant receptor-mediated sperm activation in disease vector mosquitoes

Contact Info

Product

Resources

About

Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca²⁺ Imaging