Sperizin Is a Murine RING Zinc-Finger Protein Specifically Expressed in Haploid Germ Cells

Fujii, Takayuki; Tamura, Kazutoshi; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Yomogida, Kentaro; Tanaka, Hiromitsu; Nishimune, Yoshitake; Nishimura, Hiroshi; Abiko, Yoshimitsu

doi:10.1006/geno.1998.5738

Cited by 40 publications

(45 citation statements)

References 26 publications

(25 reference statements)

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“…Further studies will be warranted to elucidate the role of DHA in sterol metabolism in testis. Sperizin is a protein highly expressed in spermatids and may act as ubiquitin ligase ( 29,30 ). A study reported complete abolition of sperizin RNA in the testis of D6D-null mice and suggested an arrest of the gene expression sequence at the spermiogenesis stage ( 20 ).…”

Section: Testis Dha and Cholesta-35-diene Were Restored By Dietary Dhamentioning

confidence: 99%

Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice

Roqueta‐Rivera

Stroud

Haschek

et al. 2010

Journal of Lipid Research

130

116

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This article is available online at http://www.jlr.org Supplementary key words essential fatty acids • arachidonic acid • highly unsaturated fatty acids • very-long-chain polyunsaturated fatty acids • choelsta-3,5-diene • male reproduction • spermiogenesis Delta-6 desaturase (D6D) is the fi rst and rate-limiting enzyme for highly unsaturated fatty acid (HUFA) synthesis that consists of a series of elongation and desaturation reactions ( 1 ). The dietary essential fatty acids 18:2n-6 (linoleic acid) and 18:3n-3 ( ␣ -linolenic acid) are substrates for D6D and precursors of physiologically important HUFAs, such as 20:4n-6 [arachidonic acid (AA)], 22:5n6 [docosapentaenoic acid (DPAn6)], and 22:6n3 [docosahexaenoic acid (DHA)]. D6D is also required for the fi nal desaturation step for the synthesis of DPAn6 and DHA.These HUFAs are present in high concentration in testes and sperm of mammals. DPAn6, a HUFA derived from AA, dramatically increases in rat testes during the sexual maturation stage ( 2 ). In mice, AA, DPAn6, and DHA are abundant in membrane phospholipids of round spermatids ( 3 ) and mature mouse spermatozoa ( 4 ), suggesting an important role for these fatty acids for proper spermatogenesis. In humans, DHA is the main HUFA in sperm ( 5 ). DHA is specifi cally high in the sperm tail when compared with the sperm head in monkeys ( 6 ), implying a role of DHA in sperm tail function. AA may also have a role in male fertility as a precursor to eicosanoids. Prostaglandin E2, for example, has been shown to increase sperm motility ( 7 ), while inhibition of cycloxygenase-2 in mouse vas deferens results in a decrease of sperm motility and fertilAbstract Delta-6 desaturase-null mice ( ؊ / ؊ ) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The ؊ / ؊ males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type ( Abbreviations: AA, arachidonic acid; CD, cholesta-3,5-diene; D6D, ⌬ 6 desaturase; DHA, docosahexaenoic acid; DPAn6, docosapentaenoic acid; FAME, fatty acid methyl esters; HUFA, highly unsaturated fatty acid; VLPUFA, very-long-chain polyunsaturated fatty acid.

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Section: Testis Dha and Cholesta-35-diene Were Restored By Dietary Dhamentioning

confidence: 99%

Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice

Roqueta‐Rivera

Stroud

Haschek

et al. 2010

Journal of Lipid Research

130

116

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“…Previously, we isolated cDNA clones that are specifically expressed in mouse haploid germ cells from the subtracted cDNA library (7). Many of these haploid germ cell-specific genes are thought to be involved in the differentiation process of spermiogenesis and/or in sperm function (7)(8)(9)(10)(11)(12)(13)(14)(15). We report here the characterization of a novel RBCC (RING finger, B-box type zinc finger, and coiled-coil domain) motif family gene named haprin (from haploid germ cell-specific RBCC protein), which was cloned from a subtracted cDNA library.…”

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confidence: 99%

Haprin, a Novel Haploid Germ Cell-specific RING Finger Protein Involved in the Acrosome Reaction

Kitamura

Tanaka

Nishimune

2003

Journal of Biological Chemistry

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The acrosome reaction (i.e. the exocytosis of the sperm vesicle) is a prerequisite for fertilization, but its molecular mechanism is largely unknown. We have identified a cDNA clone for a gene named haprin, which encodes a haploid germ cell-specific RING finger protein. This protein is a novel member of the RBCC (RING finger, B-box type zinc finger, and coiled-coil domain) motif family that has roles in several cellular processes, such as exocytosis. It is transcribed exclusively in testicular germ cells after meiotic division. Western blot and immunohistochemical analyses showed the molecular weight of Haprin protein to be M r ϳ82,000. It was localized in the acrosomal region of elongated spermatids and mature sperm and was not present in acrosome-reacted sperm. The specific antibody against the RING finger domain of Haprin inhibited the acrosome reaction in permeabilized sperm. These results indicated that the novel RBCC protein Haprin plays a key role in the acrosome reaction and fertilization.

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“…This observation and the intriguing specific expression of ORF162 in male sexual organs suggest that ORF162 is most likely involved in a male-specific function, such as spermatogenesis. Indeed, a RING finger protein involved in spermatogenesis has been identified in mouse (31). Further in-depth analysis of the ORF162 transcription pattern and protein analyses are required to identify its function in M. polymorpha.…”

Section: Discussionmentioning

confidence: 99%

The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene

Okada

Sone

Fujisawa

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of Ϸ70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2-3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human.dioecism ͉ sex chromosome ͉ FISH ͉ RING finger motif ͉ convergent evolution

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Sperizin Is a Murine RING Zinc-Finger Protein Specifically Expressed in Haploid Germ Cells

Cited by 40 publications

References 26 publications

Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice

Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice

Haprin, a Novel Haploid Germ Cell-specific RING Finger Protein Involved in the Acrosome Reaction

The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene

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