Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence

Firooziyan, Samira; Djadid, Navid Dinparast; Gholizadeh, Saber

doi:10.1186/s12936-018-2523-y

Cited by 24 publications

(52 citation statements)

References 26 publications

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“…stephensi, the Type form is reported as main malaria vector in urban areas of Indian subcontinent [24]. On the other hand, many reports have mentioned all these three forms as e cient vectors for malaria transmission in Iran [6,16]. Despite of e cient controlling strategies for malaria, this mosquito is increasing its geographic range.…”

Section: Discussionmentioning

confidence: 99%

“…8). Whereas like the tree constructed in previous studies [15,16] An. stephensi sibling A and B were placed in the separate clades (Fig.…”

Section: Genetic Analysismentioning

confidence: 92%

“…An. stephensi sibling species sequences, A (KJ557463), B (KJ557452), C (KJ557455) [16] used as representative for and sequence comparisons and phylogenetic tree construction.…”

Section: Ampli Cation Of Coi Coii Its2 and Obp1 Fragmentsmentioning

confidence: 99%

“…Mitochondrial oxidases subunit 1 and 2 (COI and COII), and ribosomal internal transcribed spacer 2 (rDNA-ITS2) and domain-3 (D3) loci are the major molecular markers used in literature, but none of them have distinguished the three subtypes of An. stephensi [16]. Moreover, overlapping in number of ridges on the surface of eggs (used as morphological keys) may create ambiguity in identi cation [3].…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Characterization of ‘var. mysorensis’ form of Anopheles Stephensi

Khan¹,

Gholizadeh²,

Zhang³

et al. 2020

Preprint

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Background: Anopheles stephensi Listen (1901) is the major malaria vector in the Asia, and recently in some regions of Africa. This species includes three biological forms, namely “type”, “intermediate” and “mysorensis” with varying degree of vector competence for malaria parasites. To recognize these siblings of An. stephensi lab strain, we used the morphological features of eggs and several genetic markers i.e. Obp1 (odorant binding protein), mitochondrial oxidases subunit 1 and 2 (COI and COII), nuclear internal transcribed spacer 2 locus (ITS2). Methods: Eggs were collected from individual mosquito (n = 50) and observed for the number of ridges under stereomicroscope. DNA was extracted from female mosquitoes. After amplifying fragments by using different genetic markers (Obp1, COI, COII and ITS2), the PCR products were purified and sequenced using Sanger Sequence Technology. Phylogenetic analysis was performed after aligning query sequences against the submitted sequences in GenBank using bioinformatics software. Results: The range of ridges number on each egg float was 12-13 that corresponds to the mysorensis form. Sequence analysis for COI, COII and ITS2 demonstrated 100%, 99.46% and 99.29% similarity of our species with other Chinese, Indian and Iranian strains of An. stephensi. All the sequences of Obp1 intron I region matched 100% with the previously submitted sequences for An. stephensi sibling C (mysorensis form) from Iran and Afghanistan.Conclusion: The current study elaborately describes the morphological and molecular details (sequence data) of the ‘mysorensis’ form of An. stephensi that could be helpful in elucidating its classification and also in its diﬀerentiation from other biotypes of the same and other similar anophelines species. Our findings confirmed OBP1 as the only genetic marker that successfully recognized our lab strain (mysorensis form/ sibling C). Thus using OBP1 (alone) may be very phenomenal in similar studies in the future. Future studies should involve the development of appropriate and reliable molecular keys (for sibling species identification) complementary to morphological keys.

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Section: Discussionmentioning

confidence: 99%

“…8). Whereas like the tree constructed in previous studies [15,16] An. stephensi sibling A and B were placed in the separate clades (Fig.…”

Section: Genetic Analysismentioning

confidence: 92%

“…An. stephensi sibling species sequences, A (KJ557463), B (KJ557452), C (KJ557455) [16] used as representative for and sequence comparisons and phylogenetic tree construction.…”

Section: Ampli Cation Of Coi Coii Its2 and Obp1 Fragmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Characterization of ‘var. mysorensis’ form of Anopheles Stephensi

Khan¹,

Gholizadeh²,

Zhang³

et al. 2020

Preprint

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“…Moreover, based on the nucleotide sequence variation of intron I Asteobp1 gene in different populations, An. stephensi may prove to be a complex species [40].…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis

Ghavami

Khoeini

Djadid

2020

Malar J

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Background: Anopheles maculipennis complex, the historic vector of malaria, causes serious medical problems worldwide and exhibits different behaviours. Studying the odorant-binding proteins (OBPs), which influence the chemosensory system and behavioural responses, is essential to understanding the population structure and developing effective control measures against this vector. The present study was designed to identify and analyse the obp1 gene in An. maculipennis. Methods: Adults of An. maculipennis sensu stricto were collected in Zanjan Province, northwest of Iran, and gDNAs of female mosquitoes were extracted. Fragments of An. maculipennis obp1 (Amacobp1) gene were amplified using degenerate and specific primers, and some of amplicons were selected for sequencing. Results: Analysis of amplified products identified that the sequence of Amacobp1 gene was 1341 bp long. This gene contains three exons (5′, internal, and 3′of 160, 256, and 18 bp, respectively) and encodes 144 amino acids. The sizes of introns I and II in deduced gene are 268 and 358 nucleotides, respectively. The amino acid sequence in the C-terminal of AmacOBP1 is similar to that of major malaria vector Anopheles species. However, its N-terminal has a specific signal peptide with 19 amino acids. This peptide is conserved in different studied populations, and its sequence of amino acids shows the most variation among anopheline species. Conclusions: Degenerate primers in this study are suggested for studying obp1 gene in Anopheles species. Ama-cobp1 gene is proposed as a molecular marker for the detection of intraspecific ecotypes and diagnosis of different species within Maculipennis Group. Moreover, the N-terminal of AmacOBP1 peptide is recommended as a molecular marker to identify the Amacobp1 expression patterns in different chemosensory organs for assessing the molecular mechanisms and developing novel behavioural disturbance agents to control An. maculipennis.

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Anopheles stephensi Liston 1901: Origin and Chorogeography—A New Hypothesis

Tyagi¹

2023

Desert Malaria

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Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence

Cited by 24 publications

References 26 publications

Morphological and Molecular Characterization of ‘var. mysorensis’ form of Anopheles Stephensi

Morphological and Molecular Characterization of ‘var. mysorensis’ form of Anopheles Stephensi

Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis

Anopheles stephensi Liston 1901: Origin and Chorogeography—A New Hypothesis

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