Spectrum of Hemoglobinopathies in West Bengal, India: A CE-HPLC Study on 10407 Subjects

Mukhopadhyay, Debanjan; Kaushik, Sushmita; Sengupta, Moumita; Mitra, Sumit; Datta, Chhanda; Mitra, Pinaki

doi:10.1007/s12288-014-0373-5

Cited by 13 publications

(12 citation statements)

References 8 publications

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“…In contrary to these findings, mean RDW was found to increase in all the subjects with Haemoglobin variants. The same findings were also reported by Baruah et al 13] and Mukhopadhyay et al [14] . Increase in RDW is not a usual finding of pure haemoglobinopathies.…”

Section: Discussionsupporting

confidence: 89%

A Study on Variants of Haemoglobin in Barak Valley, Assam

Hmar¹,

Nath²,

Barman³

2016

IOSR

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Section: Discussionsupporting

confidence: 89%

A Study on Variants of Haemoglobin in Barak Valley, Assam

Hmar¹,

Nath²,

Barman³

2016

IOSR

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“…Sachdev et al [26] , Jain et al [30] and Mukhopadhyay et al [29] found 12.57%, 29.3 % and 14.5% abnormal hemoglobin fractions respectively. Sachdev et al [26] , Rao et al [27] , Dolai et al [28] and Mukhopadhyay et al [29] found beta thalassemia trait cases to be 8.9 %,18.1 %,10.38 % and 5.6 % respectively. Our findings are comparable to these different Indian studies.…”

Section: Discussionmentioning

confidence: 95%

“…Different studies by Sachdev et al [26] , Rao et al [27] , Dolai et al [28] and Mukhopadhyay et al [29] have taken 2600, 800, 35413 and 10407 cases respectively for their study. Sachdev et al [26] , Jain et al [30] and Mukhopadhyay et al [29] found 12.57%, 29.3 % and 14.5% abnormal hemoglobin fractions respectively.…”

Section: Discussionmentioning

confidence: 99%

Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Sarvaiya

Chauhan

2017

APALM

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Introduction: Hemoglobinopathies and thalassemia are hereditary disorders of hemoglobin (Hb) affecting mankind at prevalent regional level. Automated cation exchange high performance liquid chromatography is being increasingly used as the initial diagnostic method for identifying normal and abnormal hemoglobin variants. Methodology:Total 2035 sample received and studied. All samples run on cation exchange high performance liquid chromatography machine by BIO-RAD.Results: Total 386(18.96%) cases had abnormal hemoglobin fractions. Beta thalassemia major cases were 17(0.83%). Beta thalassemia intermedia cases were 4(0.19%). Sickle cell trait (heterozygous Hb S) cases were 96(4.71%). Double heterozygous for sickle cell-beta thalassemia cases were 14(0.68%). There were 06 cases (0.29%) of Hb D heterozygous. There were one each case of Hb E heterozygous and Hb E-beta thalassemia (0.04% each). Hb F was raised in 7 adult cases (0.34%). Conclusion:To conclude, cation exchange high performance liquid chromatography is less time consuming, cost effective, labor saving, reproducible, accurate, sensitive and specific method to detect hemoglobinopathies and thalassemia. Most of the abnormal cases are diagnosed with this method, with few inconclusive cases require further genetic and molecular workup.

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“…[1] At present, approximately 250 million people constituting 4.5% of the world population are carriers of a hemoglobinopathy gene. [2] India constitutes 10% of thalassemia in the world with 10,000 children being born with thalassemia major every year. The frequency range of beta thalassemia is 3.5% to 15% in Indian population.…”

Section: Introductionmentioning

confidence: 99%

“…[4] In newborns, timely detection of various abnormal Hb can prevent occurrence of more serious disorders like thalassemia major. [2] High performance liquid chromatography (HPLC) has been proved to be a sensitive, specific, and reliable alternative to electrophoresis. It helps in direct identification and quantification of both normal Hb and abnormal Hb types.…”

Section: Introductionmentioning

confidence: 99%

Spectrum of thalassemia and hemoglobinopathies in a tertiary care diagnostic center

Iqbal¹,

Tabassum²,

Chatura³

2016

JCRI

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Context: Hemoglobinopathies and thalassemias are one of the most common genetic abnormalities prevalent in India and the Middle East. Aim: This study was performed to identify the distribution of abnormal types of hemoglobin (Hb) in a tertiary care diagnostic laboratory. Materials and Methods: An observational study was conducted in the Department of Hematology in a tertiary care diagnostic lab of South India to know the prevalence of hemoglobinopathies and thalassemia using high performance liquid chromatography (HPLC) as the diagnostic method. Results: A total of 518 samples were received over a period of 6-month. All 518 samples were processed for HPLC. 407 samples were normal and 111 samples showed abnormal Hb variants. Sickle cell trait (HbS heterozygous) was diagnosed in 56 (10.8%) cases, beta thalassemia minor in 40 (7.72%) cases, and sickle cell disease (HbS homozygous) in 12 (2.5%) cases, thalassemia major in 2 (0.38%) cases and 1 (0.2%) case of hereditary persistence of fetal hemoglobin. Conclusion: One of the best methods emerging for screening and detection of various hemoglobinopathies is HPLC. This study showed that CE-HPLC is a reliable tool in diagnosing the presence of abnormal Hb in suspected cases on routine hematology.

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Spectrum of Hemoglobinopathies in West Bengal, India: A CE-HPLC Study on 10407 Subjects

Cited by 13 publications

References 8 publications

A Study on Variants of Haemoglobin in Barak Valley, Assam

A Study on Variants of Haemoglobin in Barak Valley, Assam

Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Spectrum of thalassemia and hemoglobinopathies in a tertiary care diagnostic center

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