Spectrum of Bacterial Pathogens from Urinary Infections Associated with Struvite and Metabolic Stones

Abstract

Objective: The purposes of this multi-center study were to evaluate the rate of infection stones and to evaluate the urine cultures of patients with infection stones. Materials: Charts of adulpatients with urinary stones were reviewed and data on stone analyses and urine cultures were collected. Results: In total, 1204 renal stone formers (RSFs) from 10 countries were included (776 males, 428 females). Fifty-six patients (4.6%) had struvite stones. The highest frequency of struvite stones was observed in India (23%) and Pakistan (18%). Lower rates were reported in Canada (2%), China (3%), Argentina (3%), Iraq (3%), Italy (3.5%) and Poland (3%), and intermediate rates in Egypt (5.5%) and Bulgaria (5.4%). Urine cultures were retrieved from 508 patients. Patients with struvite stones had a positive culture in 64.3% of the samples and patients with other stones, in 26.7%. In struvite stones, the most common isolates were Escherichia coli (27.7%) and Proteus spp. (27.7%), followed by Klebsiella spp. (16.7%); in other types of stone, it was Escherichia coli (47.6%), followed by Gram-positive bacteria (14.0%) Conclusions: The struvite stone composition was associated with a urinary infection, although an infection was not demonstrable with a conventional midstream urine culture in about 30%.

Microbial Detection and Antibiotic Susceptibility patterns of Clinical Isolates from Women With Urinary Tract Infection in AL-Nasiriyah City/Iraq

“…The high frequency of Staphylococcus spp. in our data is concurred with the study reported by [35] and [36], while our finding's contradicts with those of [37]. Moreover, this study showed that Staphylococcus aureus are the most common etiological agents causing UTIs in current study.…”

“…One-hundred and fifty samples included of patients women and control group have been divided into four age groups in this study. The age group of females were (15)(16)(17)(18)(19)(20)(21)(22)(23)(24), (25)(26)(27)(28)(29)(30)(31)(32)(33)(34), (35)(36)(37)(38)(39)(40)(41)(42)(43)(44), and (above 44 years). The study recorded the majority of both patients and control group were in the age (25-34) second group 46.3% and 38.10% respectively.…”

Metagenome-based microbial community analysis of urine-derived fertilizer

“…The re-emergence of the genus Pseudomonas in the struvite sample after its marked decrease in the stored urine can be explained based on a recent work on the bacterial pro le of kidney stones which revealed the presence of Pseudomonas in struvite stone (Halinski et al, 2023). The increase in abundance of Shewanella in the struvite sample can be attributed to the study by (Luo et al, 2018) which revealed the potential of Shewanella oneidensis strain as an inducer of struvite mineralization.…”

“…With the emergence of culture-independent approaches and the subsequent human microbiome project (Turnbaugh et al, 2007), studies revealed the presence of a unique microbiota in the urinary tract system (reviewed in Zuber et al, 2023), the microbial structure and composition is also reported to be subject to change with age, sex and health status (Halinski et al, 2023. Next-Generation Sequencing (NGS) targeting 16S rRNA was the most widely used approach to determine the bacterial portion of the urinary microbiome (Perez-Carrasco et al, 2021, Siddiqui et al, 2011, Lahr et al, 2016.…”