Spectrum of bacterial keratitis in North India: A retrospective analysis of six years at a tertiary care eye center

Abstract

Purpose: To analyze the pattern of bacterial pathogens causing infective keratitis and their resistance to the recommended antibiotics over six years. Methods: It was a retrospective study of 9,357 cases of bacterial keratitis from January 2015 to December 2020, at a tertiary care ophthalmic center. A total of 9,547 corneal specimens were obtained from the study subjects. Demographic details of the patients, pathogenic bacteria isolated, and their antimicrobial susceptibility were noted and analyzed. Results: Bacterial pathogens were identified in 23.52% of the specimens. The most common isolates were coagulase-negative Staphylococci (60.75%), followed by Pseudomonas aeruginosa (14.23%), Staphylococcus aureus (13.92%), gram negative bacilli of the family Enterobacterales (8.64%), Streptococcus spp. (1.72%), Acinetobacter spp. (0.13%), and other non-fermenting gram-negative bacilli (0.57%). In Staphylococci , 55–80% of isolates were resistant to erythromycin, and 40–70% to fluoroquinolones, while no resistance was observed against vancomycin. 40–60% of isolates of P. aeruginosa were resistant to cephalosporins, 40–55% to fluoroquinolones, and 30–60% to aminoglycosides. Also, 40–80% of isolates of Enterobacterales were resistant to cephalosporins, and 50–60% to fluoroquinolones. Most gram-negative isolates were susceptible to carbapenems and polymyxin B. Conclusion: To the best of our knowledge, our study is the largest compilation of microbiological profile of bacterial keratitis from North India. It highlights the current trend of the bacterial pathogens that cause infectious keratitis. Staphylococci and Pseudomonas were found to be the most common pathogens. Increased resistance was seen against some of the commonly prescribed empirical antibiotics. Such evidence is useful for restructuring the empirical prescription practices from time to time.

A comparison of antimicrobial regimen outcomes and antibiogram development in microbial keratitis: a prospective cohort study in Alexandria, Egypt

“…We observed similar results to previous studies conducted in Alexandria and North India reporting that PA was the second most common bacteria isolated in MK and the most prevalent GN causative agent [ 33 , 34 ]. Nevertheless, a study conducted in Nottingham, UK, during 2015 to 2019, reported that PA was the most common isolated bacteria, followed by Staphylococci spp.…”

Spectrum and antibiotic sensitivity of bacterial keratitis: a retrospective analysis of eight years in a Tertiary Referral Hospital in Southwest China

“…Poorly controlled infection can result in progressive tissue destruction and severe visual impairment ( Durand et al., 2021 ). Common microorganisms responsible for bacterial keratitis include S. aureus , coagulase-negative staphylococci (CoNS), and S. pneumoniae ( Prokosch et al., 2012 ; Ahmed et al., 2022 ; Zhang et al., 2022 ). Corneal ulcers caused by bacterial infestation are often associated with persistent chronic ocular surface diseases, long-term corneal contact lens wear, eye trauma, and systemic or local corneal immunosuppression ( Amarasekera et al., 2019 ; Durrani et al., 2019 , 2020 ; Verner et al., 2020 ).…”