Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora

Chen, Yinrui; Pan, Guoji; Xu, Wen-Fen; Sun, Qingwen; Wang, Bo; Zhang, Ye; Yang, Tianjin

doi:10.1016/j.jchromb.2020.121970

Cited by 32 publications

(19 citation statements)

References 26 publications

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“…In addition, due to the hydrophobicity of DPPH, it may affect the scavenging ability of hydrophilic compounds, so the anti-oxidant ability can be fully evaluated by combining with water-soluble ABTS [ 30 ]. These several measurement methods are complementary, and the measurement method is simple and fast [ 31 ], so these several indicators are used to evaluate the antioxidant capacity of total flavonoids before and after purification. L-ascorbic acid was chosen as the positive control because it has a strong antioxidant effect, which has been confirmed in many in vivo and in vitro experiments [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Adsorption and Desorption Characteristics of Total Flavonoids from Acanthopanax senticosus on Macroporous Adsorption Resins

Wang

Chu

et al. 2021

Molecules

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We examined the application of six different resins with the aim of selecting a macroporous resin suitable for purifying Acanthopanax senticosus total flavonoids (ASTFs) from Acanthopanax senticosus crude extract (EAS) by comparing their adsorption/desorption capacities, which led to the selection of HPD-600. Research on the adsorption mechanism showed that the adsorption process had pseudo-second-order kinetics and fit the Freundlich adsorption model. Moreover, the analysis of thermodynamic parameters indicated that the adsorption process is spontaneous and endothermic. The optimal conditions for purification of ASTFs were determined as sample pH of 3, 60% ethanol concentration, and 3 BV·h−1 flow rate, for both adsorption and desorption, using volumes of 2.5 and 4 BV, respectively. The application of macroporous resin HPD-600 to enrich ASTFs resulted in an increase in the purity of total flavonoids, from 28.79% to 50.57%. Additionally, the antioxidant capacity of ASTFs was higher than that of EAS, but both were lower than that of L-ascorbic acid. The changes in ASTFs compositions were determined using ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS), with the results illustrating that the levels of seven major flavonoids of ASTFs were increased compared to that in the crude extract.

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Section: Resultsmentioning

confidence: 99%

Adsorption and Desorption Characteristics of Total Flavonoids from Acanthopanax senticosus on Macroporous Adsorption Resins

Wang

Chu

et al. 2021

Molecules

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“…For example, the two methods (PPMCC and MLRA) we used in this work indicated that both could establish the spectral correlation model to determine the chromatographic peaks which are closely related to the antioxidant activity. The two antioxidant methods mentioned in this work were also used in a lot of research [ 51 – 53 ]. The results showed that the methods were reliable for the determination of antioxidant activity in vitro .…”

Section: Discussionmentioning

confidence: 99%

Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription

Zhang

Zhao

et al. 2021

Journal of Analytical Methods in Chemistry

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Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.

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“…Spectrum‐effect relationship analysis can combine the chromatographic fingerprint with pharmacological efficacy through multiple statistical analysis methods. This helps to assess the internal quality and find the major active constituents involved in clinical efficacy 4,5,26 . Spectrum‐effect relationship has been successfully applied to analyse a variety of bioactive constituents in TCM.…”

Section: Discussionmentioning

confidence: 99%

Spectrum‐effect relationship between UPLC fingerprints and anti‐lung cancer effect of Panax ginseng

Zhou

Liu

Zhang

et al. 2020

Phytochemical Analysis

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Objectives Lung cancer has the highest mortality rate among the various types of cancer. Panax ginseng (C. A. Mey). is a popular anti‐cancer herbal supplement. The quality control of ginseng is crucial to ensure its clinical efficacy. This study aimed to establish new quality control methods for ginseng and to identify its main active components responsible for lung cancer treatment. Methods Ultra‐high‐performance liquid chromatography (UPLC) was used to establish fingerprints of 18 batches of ginseng. CCK‐8 test was performed to evaluate the inhibitory activity of ginseng on Lewis lung cancer (LLC) cells. The spectrum‐effect relationship analysis of ginseng was assessed by canonical correlation analysis (CCA) and bioactivity validation. Key findings Six common peaks were identified and the variation coefficients were determined. The 18 batches of ginseng inhibited the proliferation of LLC cells to different degrees, showing different half maximal inhibitory concentration (IC50) values. Spectrum‐effect relationship analysis showed that ginsenoside Ro is the main anti‐proliferative constituent of LLC cell. Conclusions Spectrum‐effect relationship is suitable for quality control of ginseng used for lung cancer. It is also effective in discovering the active ingredients related to the clinical efficacy of traditional Chinese medicine.

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Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora

Cited by 32 publications

References 26 publications

Adsorption and Desorption Characteristics of Total Flavonoids from Acanthopanax senticosus on Macroporous Adsorption Resins

Adsorption and Desorption Characteristics of Total Flavonoids from Acanthopanax senticosus on Macroporous Adsorption Resins

Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription

Spectrum‐effect relationship between UPLC fingerprints and anti‐lung cancer effect of Panax ginseng

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