Spectrum–effect relationship study between HPLC fingerprints and antioxidant of honeysuckle extract

Liu, Hongyan; Zhu, Shu; Liu, Qian; Yong-qing, Zhang

doi:10.1002/bmc.4583

Cited by 24 publications

(21 citation statements)

References 14 publications

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“…The spectrum-effect relationship is a new and reliable method that can combine chromatographic fingerprint with pharmacological effects by multiple chemometrics. This method could help to explore the correlations between bioactive components and efficacy, and find the major bioactive ingredients in herbal medicines (Yang et al, 2016;Liu et al, 2019). The spectrumeffect relationship method has been successful used to discover varieties of bioactive compounds in herbal medicines.…”

Section: Discussionmentioning

confidence: 99%

“…(Jiang et al, 2010;Quan et al, 2019;Gong et al, 2020). To overcome this shortcoming, a new and reliable method named spectrum-effect relationships analysis was employed to investigate the correlations between pharmacodynamics and chemical components of herbal medicines (Yang et al, 2016;Liu et al, 2019). Chromatographic fingerprints could characterize the chemical components of herbal medicines, which is an effective method for uniformity and quality evaluation of herbal medicines.…”

Section: Introductionmentioning

confidence: 99%

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Screening of Anti-Lipase Components of Artemisia argyi Leaves Based on Spectrum-Effect Relationships and HPLC-MS/MS

et al. 2021

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Pancreatic lipase is a key lipase for triacylglyceride digestion and absorption, which is recognized as a promising target for treatment of metabolic disorders. Natural phytochemicals are hopeful sources for pancreatic lipase inhibitors. The leaves of Artemisia argyi H.Lév. and Vaniot (AL) is commonly used as herbal medicine or food supplement in China and other Asian countries for hundreds of years. AL mainly contains essential oils, phenolic acids, flavonoids and terpenoids, which exhibit many pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial, analgetic, anti-cancer, anti-diabetes and immunomodulatory effects. However, the anti-lipase activity of AL was lack of study and the investigation of anti-lipase ingredients from AL was also insufficient. In the present study, the anti-lipase activity of AL was evaluated in vitro and the potentially pancreatic lipase inhibitors of AL were investigated. High performance liquid chromatography was used to establish fingerprints of AL samples, and fifteen peaks were selected. The anti-lipase activities of AL samples were evaluated by a pancreatic lipase inhibition assay. Then, the spectrum-effect relationships between fingerprints and pancreatic lipase inhibitory activities were investigated to identify the anti-lipase constitutes in AL. As the results, four caffeoylquinic acids, which were identified as neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, and isochlorogenic acid A by high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, were selected as potential pancreatic lipase inhibitors in AL. Moreover, anti-lipase activity assessment and molecular docking study of the four compounds were performed to validate the potential lipase inhibitors in AL. The results revealed that the four caffeoylquinic acids in AL as bioactive compounds displayed with anti-lipase activity. The present research provided evidences for the anti-lipase activity of AL, and suggested that some bioactive compounds in AL could be used as lead compounds for discovering of new pancreatic lipase inhibitors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening of Anti-Lipase Components of Artemisia argyi Leaves Based on Spectrum-Effect Relationships and HPLC-MS/MS

et al. 2021

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“…In recent years, chemometric analysis has shown great potential and been increasingly used to seek out the key antioxidant components from complex plant extracts. [40][41][42][43] For example, Chen et al…”

Section: Chemometric Analysismentioning

confidence: 99%

Correlations between phytochemical fingerprints of Moringa oleifera leaf extracts and their antioxidant activities revealed by chemometric analysis

Chen

2020

Phytochemical Analysis

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Introduction Moringa oleifera Lam. is widely cultivated and applied in tropical and subtropical areas. Numerous studies have been focused on the antioxidant capacity of M. oleifera leaves, but its correlated bioactive phytochemicals remain elusive. Objective In order to search for the corresponding chemical compounds from M. oleifera leaves responsible for their antioxidant activity, the correlations between phytochemical fingerprints of 15 batches of M. oleifera leaves and their antioxidant activities were investigated by using chemometric analysis. Material and Methods Fifteen batches of M. oleifera leaves were extracted with 90% ethanol solution, and their phytochemical fingerprints and antioxidant activities were estimated by using high‐performance liquid chromatography‐ultraviolet‐electrospray ionisation tandem mass spectrometry (HPLC‐UV/ESI‐MS/MS), and three detected methods, namely 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, 2,2′‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS) assay and ferric‐reducing antioxidant power (FRAP) assay, respectively. Chemometric analysis was then applied to reveal the correlations between their phytochemical fingerprints and corresponding antioxidant capacity. Results Fifteen M. oleifera leaf extracts exhibited strong antioxidant activities, in which 24 common compounds were identified by LC–MS. Furthermore, the partial least squares (PLS) analysis indicated that compounds 14, 16, 18 and 23 were the main potential effective components in at least two antioxidant tests. They were identified as kaempferol 3‐O‐rutinoside, quercetin 3‐O‐(6″‐malonyl‐glucoside), kaempferol 3‐O‐glucoside, and quercetin derivative, respectively. Conclusion The correlations between phytochemical fingerprints of M. oleifera leaf extracts and their corresponding antioxidant capacities were revealed by chemometric analysis, which provides an alternative method for screening for potential bioactive compounds with antioxidant capacity from M. oleifera leaves.

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“…Spectrum-effect relationships have been widely used to screen the active compounds of TCMs by combining chromatographic fingerprint of TCMs with their biological activity. Chromatographic fingerprints of traditional Chinese medicines (TCMs) contain a large number of information and could express the chemical characteristics of samples integrally [28,29]. In recent years, spectrum-effect relationships were often being used to assess the quality control of TCMs [30].…”

Section: Introductionmentioning

confidence: 99%

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

You

Zhong

et al. 2021

Journal of Chemistry

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Crocus sativus L. is commonly used as functional food and medicinal herb in traditional Chinese medicine. In this study, the spectrum–effect relationship was established between HPLC fingerprints and in vitro antioxidant activity of saffron to improve the quality evaluation method of saffron. The fingerprints of 21 batches of saffron collected from different regions were assessed, and the data were further analyzed by chemometric methods, including similarity analysis, hierarchical clustering analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The spectrum–effect relationship between fingerprints and antioxidant effect of saffron was analyzed by grey relational analysis and partial least square methods to figure out the antioxidant component of saffron. Thirteen common peaks of 21 batches of saffron were included in the analysis, and peak 3 (picrocrocin), peak 7 (crocin I), and peak 10 (crocin II) were identified as the main active components responsible for antioxidant efficacy. Besides, a multi-index quality control method was developed for simultaneous determination of these three antioxidant components in saffron. Taken together, this study provided new strategies for the quality control and the development of new bioactive products of saffron in the future.

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Spectrum–effect relationship study between HPLC fingerprints and antioxidant of honeysuckle extract

Cited by 24 publications

References 14 publications

Screening of Anti-Lipase Components of Artemisia argyi Leaves Based on Spectrum-Effect Relationships and HPLC-MS/MS

Screening of Anti-Lipase Components of Artemisia argyi Leaves Based on Spectrum-Effect Relationships and HPLC-MS/MS

Correlations between phytochemical fingerprints of Moringa oleifera leaf extracts and their antioxidant activities revealed by chemometric analysis

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

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