Spectrum–effect relationship between HPLC fingerprints and hypolipidemic effect of Curcuma aromatica

Abstract

Curcuma aromatica is used as a traditional Chinese medicine, and it is mainly distributed in Guangxi, China. In this study, 10 batches of C. aromatica were collected from different origins in Guangxi. The fingerprints were established by HPLC technique to investigate the quality stability of C. aromatica. The spectrum-effect relationship between HPLC fingerprints and hypolipidemic effect of C. aromatica was assessed by similarity analysis, gray relational analysis and multiple linear regression analysis. From the results, the similarity values between each batch of C. aromatica and reference fingerprint were >0.880, indicating the good quality stability of the 10 batches of C. aromatica. Twenty common peaks were selected as the fingerprints to evaluate the quality and hypolipidemic effect of C. aromatica. The results of spectrum-effect relationship showed that peaks 10, 18, 13, 15 and 17 in the fingerprints were closely related to hypolipidemic effect. This study successfully established the spectrum-effect relationship between HPLC fingerprints and hypolipidemic effect of C. aromatica, which provided methods for quality control and more effectively studies on bioactive compounds of C. aromatica. It could also provide a new simple and effective method for utilizing the fingerprints to optimize the Chinese prescription and develop traditional Chinese medicine.

Comparative study of chemical composition and active components against α‐glucosidase of various medicinal parts of Morus alba L.

“…Spectrum–effect relationship analysis is an emerging approach to facilitating the bioactivity‐relevant quality control of herbal medicines. There is no doubt that the spectrum–effect relationship approach fits very well with the holistic mode of herbal medicines, eliciting the pharmacological efficacy via several components (Liu, Wu, Huang, Liu, & Feng, ; Shi et al, ; Zhang, Chen, Yang, & Shi, ). In the present study, we established a spectrum–effect relationship model to identify a set of combinatorial marker compounds that might be representative of the bioactivity of Cortex Mori, Ramulus Mori, Folium Mori and Fructus Mori.…”

Stability of Alkaloids during Drying Process and Their Effect on Anticoagulating Activity of Uncariae Ramulus Cum Uncis

“…Chromatographic separation was conducted on a ZORBAX SB‐C 18 column (150 × 4.6 mm, 5 μ m), and the temperature was maintained at 30°C [7]. The detection wavelength was at 245 nm.…”

“…Analysis was conducted for both the standard solutions and sample solutions obtained in Sections 3.1.3 and Preparation of Sample Solution in Different Dry Conditions in Section 3.1.4, respectively at the 80th min to determine the precision [7]. Through three injections of one sample, the repeatability was evaluated and each sample solution was detected three times in parallel.…”

“…For instance, HPLC plays an important role in determining contents, analyzing constituents of many compounds, and obtaining the HPLC spectrum of CHM, which are deemed as useful in the identification and quality control of botanical medicines [4–6]. However, it was found that multicomponents instead of one contribute to the active compounds of CHM [7]. Xu's group suggested that the HPLC spectrum can help analyze the whole fingerprint of CHM, which illustrates the detailed compounds of a CHM [8].…”

Identification of blood-activating components from Xueshuan Xinmaining Tablet based on the spectrum–effect relationship and network pharmacology analysis

“…According to the Similarity Evaluation System for Chromatographic Fingerprint of TCM (version 2012A; Beijing, China), the ngerprints were automatically matched and established. 17 Chromatograms of S 1 -S 10 samples, including PA and t R , were put into the Analytical Instrument Association (AIA) form (*.cdf). Subsequently, a reference ngerprint was automatically generated by comparing S 1 -S 10 samples based on the median method, and the similarity (SA) between the chromatogram of the reference ngerprint and each sample chromatogram was calculated with the soware.…”