Spectrum and susceptibility of preoperative conjunctival bacteria

Fernández-Rubio, María-Elisa; Cuesta-Rodríguez, Teresa; Urcelay-Segura, J.L.; Cortés-Valdés, C

doi:10.1016/j.oftale.2014.01.007

Cited by 3 publications

(6 citation statements)

References 40 publications

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“…and NFGNB in this study represents a high resistance to some current antibiotic prophylaxes for cataract surgery (Table 5). 3,5,6,8,9,20,21 In our previous study, the category smokers + exsmokers was associated with the Gram-negative bacilli group. 18 In this study, only the smoker's category is associated with Enterobacteriaceae and not with NFGNB (Table 5).…”

Section: Discussionmentioning

confidence: 80%

“…15 Our association of NFGNB, Enterococcus spp., and Enterobacteriaceae in participants aged older than 79 years is interesting of their pathogenic behavior and high antibiotic resistance, which could result in a selection of PE cases caused by these bacteria and even could determine the risk factors for a PE series. [2][3][4][5][6][7][8]17,21 For example, in a big Swedish series, with 99.3% of their patients receiving intracameral cefuroxime prophylaxis, age older than 84 years was a risk for PE, but male sex was not. 3 However, in another large French PE series with 26.5% of their patients receiving intracameral cefuroxime prophylaxis, age was not a risk for PE, but male sex was.…”

Section: Discussionmentioning

confidence: 99%

“…and NFGNB in this study represents a high resistance to some current antibiotic prophylaxes for cataract surgery (Table 5). 3,5,6,8,9,20,21…”

Section: Discussionmentioning

confidence: 99%

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Association between the clinical and demographic characteristics of patients undergoing cataract surgery and their prevalence of conjunctival bacteria

Fernández-Rubio¹,

Urcelay-Segura²,

Bellón-Cano³

et al. 2021

J Cataract Refract Surg

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Association between the clinical and demographic characteristics of patients undergoing cataract surgery and their prevalence of conjunctival bacteria

Fernández-Rubio¹,

Urcelay-Segura²,

Bellón-Cano³

et al. 2021

J Cataract Refract Surg

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“…Therefore, it is very important to maintain preoperative and postoperative asepsis of both eyelid and conjunctiva. However, not all the antibiotic prophylaxis has been reported to be clearly effective and, additionally, there is an increase of resistant bacteria that di cult the success of these strategies [24][25][26]. Therefore, apart from other prophylactic choices, periocular skin hygiene might contribute to reduce the risk of ocular infections associated to the surgery.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of daily hygiene routine using cleansing wipes in the reduction of eyelid bacterial load.

Gómez-Gómez

Méndez

Muniesa

2020

Preprint

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Background- This study aims to evaluate the efficacy of using a cleansing eyelid wipe to reduce microbial load in healthy subjects. Methods- A single-center, prospective study was conducted. Twenty healthy subjects were assigned to wipe their periocular area (eyelid, eyelashes and lid margin) twice a day with a commercial sterile wipe for 5 consecutive days. Bacterial load (total aerobic bacteria and Staphylococcus genus) was recovered from the same wipes and was measured on day 1, 3 and 5, with day 1 serving as an internal control. Both eyes were assessed independently, resulting in a total of 40 samples. Microbial evaluation was performed by culture on Tryptone Soy Agar (TSA) and Mannitol Salt Agar (MSA) plates. Results- Positive and negative controls rendered expected results thus validating this innovative extraction procedure. Microbial growth on both media revealed that the great majority of microorganisms detected belong to the genus Staphylococcus. Bacterial load reduction was significant (Mann-Whitney test p < 0.05) for both microorganisms between days 1 to 3, days 3 to 5 and days 1 to 5. Measurements at day 3 revealed a reduction on bacterial load of total aerobes and Staphylococcus spp. by 58.7% and 56.5% respectively. Moreover, on day 5, bacterial load showed a mean reduction of 80.8% and 82.7% on total aerobes and Staphylococcus spp. respectively. Furthermore, on day 5, 42.5% of the eyes showed ≥ 90% reduction in total aerobes and 42.5% for Staphylococcus spp. On the third day 25% of the volunteers showed ≥ 90% reduction of aerobes microbial load at least in one eye and the percentage was 15% for Staphylococcus spp. On the fifth day those values arise to 50% for total aerobes and 65% for Staphylococcus spp.Conclusions- The study demonstrates the efficacy of the eyelid hygiene with commercial cleansing wipes in significantly reducing (p < 0.05) bacterial load in the periocular area after 3 days of use, with further reduction on the fifth day. Therefore, these wipes could be recommended in situations in which adequate of palpebral hygiene is needed.

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“…La presencia de estas bacterias puede deberse, en parte, a que el 55 % de los participantes eran estudiantes de medicina veterinaria o a la convivencia con mascotas (dato que no se analizó en este estudio); sin embargo, estas bacterias se clasificaron como flora transitoria, ya que solo se aislaron en una muestra de cada sujeto. La convivencia con animales y la actividad que desempeñan los individuos incrementa la diversidad y la cantidad del microbioma ambiental, que probablemente incide en la comunidad de bacterias que forman parte de la flora normal de piel y mucosas (32,33 Las bacterias de la microbiota intestinal se han reportado con cierta frecuencia en la conjuntiva humana, especialmente E. coli (8,14,34); el origen de estas bacterias en la conjuntiva puede deberse a autocontagio o malos hábitos de higiene.…”

Section: Discussionunclassified

Caracterización de la microbiota conjuntival transitoria y residente de adultos jóvenes

Algarra¹,

Niño²,

Rodríguez³

2017

Cienc. Tecnol. Salud Vis. Ocul.

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Cómo citar este artículo: Martin Algarra V, Jaimes Niño V, Rodríguez MF. Caracterización de la microbiota conjuntival transitoria y residente de adultos jóvenes. Cienc Tecnol Salud Vis Ocul. 2017;15(1):37-45. doi: http://dx.doi.org/10.19052/sv.3994 RESUMEN La microbiota ocular se encuentra principalmente en la conjuntiva y está constituida por bacterias de los géneros Staphylococcus corynebacterium spp., Bacillus spp., Neisseria spp., Moraxella spp. y Streptococcus spp. Esta diversidad puede variar según las condiciones estacionales, la temperatura, la edad y la exposición ambiental. Objetivo: caracterizar la diversidad de la microbiota residente y transitoria de la conjuntiva en adultos jóvenes. Metodología: estudio observacional descriptivo de corte transversal en 67 adultos jóvenes, hombres y mujeres entre 18 y 25 años de edad, que cumplieron con los criterios de inclusión y aceptaron firmar el consentimiento informado. A cada sujeto se le tomaron dos muestras del saco conjuntival, con un intervalo de una semana. Estas se cultivaron en agar sangre y agar chocolate a 37 °C por 24 horas. Las colonias aisladas se identificaron mediante el sistema automatizado VITEK. Resultados: se determinaron 16 géneros y 29 especies diferentes. El género más frecuente fue Staphylococcus (55,6 %), seguido de Acinetobacter (12,0 %), Bacillus (7,0 %), Pasterella (4,6 %) y Escherichia (3,7 %). La microbiota residente estuvo constituida principalmente por el género Staphylococcus y sus especies más representativas fueron S. epidermidis (50,0 %) y S. lentus (13,3 %). Conclusión: existe gran diversidad de bacterias en la microbiota conjuntival, mayor en la microbiota transitoria que en la residente; probablemente, esto depende del ambiente en el que se encuentra el individuo.

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Spectrum and susceptibility of preoperative conjunctival bacteria

Cited by 3 publications

References 40 publications

Association between the clinical and demographic characteristics of patients undergoing cataract surgery and their prevalence of conjunctival bacteria

Association between the clinical and demographic characteristics of patients undergoing cataract surgery and their prevalence of conjunctival bacteria

Efficacy of daily hygiene routine using cleansing wipes in the reduction of eyelid bacterial load.

Caracterización de la microbiota conjuntival transitoria y residente de adultos jóvenes

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