Spectroscopical and mechanical characterization of normal and thalassemic red blood cells by Raman Tweezers

Luca, Anna Chiara De; Rusciano, Giulia; Ciancia, Rosanna; Martinelli, Vincenzo; Pesce, Giuseppe; Rotoli, Bruno; Selvaggi, Lara; Sasso, Antonio

doi:10.1364/oe.16.007943

Cited by 120 publications

(82 citation statements)

References 46 publications

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“…While this simplifies the full spectrum into a collection of contributing components, it can sacrifice some of the physical meaning behind these features. Spectral deconvolution has been used to analyze Raman spectra in a variety of applications, including gastric cancer [426], effects of pesticides [472], red blood cell disease [473], breast cancer [474], and stem cell differentiation [475].…”

Section: Direct Peak Analysismentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Direct Peak Analysismentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

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“…In addition, when Raman spectroscopy is combined with confocal microscopy and optical tweezers, the resultant laser tweezers Raman spectroscopy (LTRS) allows the nondestructive, noninvasive detection of biochemical processes at the single-cell level (9,10,19,46). Indeed, LTRS has been used to analyze the DPA level and the germination of individual Bacillus spores (5,19,30).…”

Section: ؊1mentioning

confidence: 99%

Characterization of Wet-Heat Inactivation of Single Spores of Bacillus Species by Dual-Trap Raman Spectroscopy and Elastic Light Scattering

Zhang

Kong

Setlow

et al. 2010

Appl Environ Microbiol

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Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELSBacterial spores of Bacillus species are formed in sporulation and are metabolically dormant and extremely resistant to a variety of harsh conditions, including heat, radiation, and many toxic chemicals (37). Since spores of these species are generally present in foodstuffs and cause food spoilage and food-borne disease (37, 38), there has long been interest in the mechanisms of both spore resistance and spore killing, especially for wet heat, the agent most commonly used to kill spores. The killing of dormant spores by wet heat generally requires temperatures about 40°C higher than those for the killing of growing cells of the same strain (37, 43). A number of factors influence spore wet-heat resistance, with a major factor being the spore core's water content, as spores with higher core water content are less wet-heat resistant than are spores with lower core water (15,25). The high level of pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) and the types of its associated divalent cations, predominantly Ca 2ϩ , that comprise ϳ25% of the dry weight of the core also contribute to spore wet-heat resistance, although how low core water content and CaDPA protect spores against wet heat is not known. The protection of spore DNA against depurination by its saturation with a group of ␣/␤-type small, acid-soluble spore proteins also contributes to spore wet-heat resistance (14,23,33,37).Despite knowledge of a number of factors important in spore wet-heat resistance, the mechanism for wet-heat killing of spores is not known. Wet heat does not kill spores by DNA damage or oxidative damage (35, 37). Instead, spore killing by this agent is associated with protein denaturation and enzyme inactivation (2, 7, 44), although specific proteins for which damage causes spore death have not been identified. Wet-heat treatment also often results in the release of the spore core's large depot of CaDPA. The mechanism for this CaDPA release is not known but is presumably associated with the rupture of the spore's inner membrane (7). In addition, the relationship between protein denaturation and CaDPA release is not clear, although recent work suggests that significant protein denaturation can occur prior to CaDPA release (7). Almost all information on spore killing by moist heat has been obtained with spore populations, and essentially nothing is known about the behavior of individual spores exposed to potentially lethal temperatures in water. Given the likely heterogeneity of spores in populations, in particular in their wetheat resistances (16,18,39,40), it could be most informative to analyze the behavior of individual spores exposed to high temperatures in water.Raman spectroscopy is widely used in biochemical studies, as this technique has high sensitivity and responds rapidly to subtle changes in molecule structure (1, 22, 31). In addition,

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“…151 Other studies explored the oxygenation capability of diseased erythrocytes: Rusciano et al explored the characterization of an erythrocyte from ␤-thalassemic patients obtained by using an optical tweezers system integrated with a Raman probe. 152 They compared the Raman spectra of single thalassemic erythrocytes to those obtained from healthy subjects, relating the observed differences to the analyzed disease. The photoinduced effects caused by the Raman and trapping laser radiation were also investigated.…”

Section: Imaging and Spectroscopy With Optical Manipulationmentioning

confidence: 99%

Light forces the pace: optical manipulation for biophotonics

2010

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Abstract. The biomedical sciences have benefited immensely from photonics technologies in the last 50 years. This includes the application of minute forces that enable the trapping and manipulation of cells and single molecules. In terms of the area of biophotonics, optical manipulation has made a seminal contribution to our understanding of the dynamics of single molecules and the microrheology of cells. Here we present a review of optical manipulation, emphasizing its impact on the areas of single-molecule studies and single-cell biology, and indicating some of the key experiments in the fields.

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Spectroscopical and mechanical characterization of normal and thalassemic red blood cells by Raman Tweezers

Cited by 120 publications

References 46 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Characterization of Wet-Heat Inactivation of Single Spores of Bacillus Species by Dual-Trap Raman Spectroscopy and Elastic Light Scattering

Light forces the pace: optical manipulation for biophotonics

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