Spectroscopic translation of cell–material interactions

Allen, Josephine B.; Liu, Yang; Kim, Young L.; Turzhitsky, Vladimir; Backman, Vadim

doi:10.1016/j.biomaterials.2006.07.002

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…The value of this powerlaw exponent was sensitive to the differentiation status of smooth muscle cells, the level of adhesion of endothelial cells on fibronectin and laminin substrates, and the extent of endothelial cell apoptosis in response to various concentrations of tumor necrosis factor-a. 12 Similar measurements have also been acquired dynamically from silk films following several mineralization cycles. 13 The intensity of the detected scattered light was highly correlated with the overall levels of mineral deposition, while the wavelength dependence of the scattered light revealed important differences in the organization of the mineral deposits, depending on the b-sheet content of the original film.…”

Section: Spectroscopic Measurements For Tissue Engineering Applicationsmentioning

confidence: 85%

“…More recently, light scattering spectroscopic measurements performed using linearly polarized light and detecting the backscattered light that has undergone only a single or very few scattering events have revealed the potential of this technique to assess important cellular and matrix features. 12,13 Specifically, it has been found that the backscattered light intensity has an inverse power law dependence as a function of wavelength consistent with scattering from a log-normal distribution of scatterers with sizes varying from a few tens to a few hundreds of nanometers. The value of this powerlaw exponent was sensitive to the differentiation status of smooth muscle cells, the level of adhesion of endothelial cells on fibronectin and laminin substrates, and the extent of endothelial cell apoptosis in response to various concentrations of tumor necrosis factor-a.…”

Section: Spectroscopic Measurements For Tissue Engineering Applicationsmentioning

confidence: 86%

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Optical Spectroscopy and Imaging for the Noninvasive Evaluation of Engineered Tissues

Georgakoudi

Rice

Hronik-Tupaj

et al. 2008

Tissue Engineering Part B: Reviews

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Optical spectroscopy and imaging approaches offer the potential to noninvasively assess different aspects of the cellular, extracellular matrix, and scaffold components of engineered tissues. In addition, the combination of multiple imaging modalities within a single instrument is highly feasible, allowing acquisition of complementary information related to the structure, organization, biochemistry, and physiology of the sample. The ability to characterize and monitor the dynamic interactions that take place as engineered tissues develop promises to enhance our understanding of the interdependence of processes that ultimately leads to functional tissue outcomes. It is expected that this information will impact significantly upon our abilities to optimize the design of biomaterial scaffolds, bioreactors, and cell systems. Here, we review the principles and performance characteristics of the main methodologies that have been exploited thus far, and we present examples of corresponding tissue engineering studies.

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Section: Spectroscopic Measurements For Tissue Engineering Applicationsmentioning

confidence: 85%

Section: Spectroscopic Measurements For Tissue Engineering Applicationsmentioning

confidence: 86%

Optical Spectroscopy and Imaging for the Noninvasive Evaluation of Engineered Tissues

Georgakoudi

Rice

Hronik-Tupaj

et al. 2008

Tissue Engineering Part B: Reviews

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“…Although it is possible to study oxygen gradients in constructs or tissues using insertion of probes, 57 this disturbs the organization of growing cells and impedes the goal of monitoring scaffold cell interactions. 58 Scaffold based oxygen sensing is investigated here with boron dyes that emit a phosphorescence and fluorescence signal whose ratio changes with oxygen concentration, to overcome this drawback for construct study. Importantly, the nanofiber morphology of the dye-polymer conjugate scaffolds, obtained with careful parameter adjustments for the low molecular weight polymer, is an excellent substrate for cell attachment and growth.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Oxygen Sensing Using Dual Emissive Boron Dye–Polylactide Nanofibers

et al. 2014

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Oxygenation in tissue scaffolds continues to be a limiting factor in regenerative medicine despite efforts to induce neovascularization or to use oxygen-generating materials. Unfortunately, many established methods to measure oxygen concentration, such as using electrodes, require mechanical disturbance of the tissue structure. To address the need for scaffold-based oxygen concentration monitoring, a single-component, self-referenced oxygen sensor was made into nanofibers. Electrospinning process parameters were tuned to produce a biomaterial scaffold with specific morphological features. The ratio of an oxygen sensitive phosphorescence signal to an oxygen insensitive fluorescence signal was calculated at each image pixel to determine an oxygenation value. A single component boron dye–polymer conjugate was chosen for additional investigation due to improved resistance to degradation in aqueous media compared to a boron dye polymer blend. Standardization curves show that in fully supplemented media, the fibers are responsive to dissolved oxygen concentrations less than 15 ppm. Spatial (millimeters) and temporal (minutes) ratiometric gradients were observed in vitro radiating outward from the center of a dense adherent cell grouping on scaffolds. Sensor activation in ischemia and cell transplant models in vivo show oxygenation decreases on the scale of minutes. The nanofiber construct offers a robust approach to biomaterial scaffold oxygen sensing.

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“…The cellular response to a biomaterial, such as proliferation and differentiation, not only depend on the chemical composition of the material, but also on the morphology of its surface [24], which plays a crucial role in determining cell behavior and influences the amount of ions released from the biomaterial [25]. that in addition to Mg some other ions such as Ca and Si can also be released to the medium, thus also contributing to the proliferation and differentiation of osteoblasts [27,28].…”

Section: Discussionmentioning

confidence: 99%

Magnesium substitution in brushite cements for enhanced bone tissue regeneration

Cabrejos-Azama

Alkhraisat

Rueda

et al. 2014

Materials Science and Engineering: C

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We have synthesized calcium phosphate cements doped with different amounts of magnesium (Mg-CPC) with a twofold purpose: i) to evaluate in vitro the osteoblast cell response to this material, and ii) to compare the bone regeneration capacity of the doped material with a calcium cement prepared without magnesium (CPC). Cell proliferation and in vivo response increased in the Mg-CPCs in comparison with CPC. The Mg-CPCs have promoted higher new bone formation than the CPC (p < 0.05). The cytocompatibility and histomorfometric analysis performed in the rabbit calvaria showed that the incorporation of magnesium ions in CPC improves osteoblasts proliferation and provides higher new bone formation. The development of a bone substitute with controllable biodegradable properties and improved bone regeneration can be considered a step towards personalized therapy that can adapt to patient needs and clinical situations.

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Spectroscopic translation of cell–material interactions

Cited by 7 publications

References 33 publications

Optical Spectroscopy and Imaging for the Noninvasive Evaluation of Engineered Tissues

Optical Spectroscopy and Imaging for the Noninvasive Evaluation of Engineered Tissues

Spatiotemporal Oxygen Sensing Using Dual Emissive Boron Dye–Polylactide Nanofibers

Magnesium substitution in brushite cements for enhanced bone tissue regeneration

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