Spectroscopic Study of Maghemite Nanoparticles Surface-Grafted with DMSA

Soler, Maria A. G.; Lima, E.C.D.; Nunes, Eloiza S; Silva, Fabio L. R.; Oliveira, A. C.; Azevedo, Ricardo Bentes; Morais, P.C.

doi:10.1021/jp1109916

Cited by 37 publications

(20 citation statements)

References 48 publications

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“…Magnetite nanoparticles were synthesized by mixing ferric and ferrous chloride aqueous solutions (2:1 molar ratio) with concentrated ammonia aqueous solution followed by vigorous stirring as described in our previous works [21][22][23]. The black magnetic precipitate was isolated by magnetic separation, washed with water and oxidized by refluxing in HCl solution under oxygen flux at 96°C, yielding a brownish colloidal suspension, indicating the efficient oxidation of magnetite to maghemite.…”

Section: Magnetic Nanoparticle Preparation and Functionalizationmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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Nanotherapy applied to cancer treatment is constantly evolving, and new approaches to current techniques, such as magnetohyperthermia, are being implemented to solve and minimize the limitations of conventional therapeutic strategies. The purpose of this study was to investigate the action of polyphosphate-coated maghemite nanoparticles (MNPs) on oral squamous cell carcinoma. Human oral cancer cells (UM-SCC14A) were incubated with MNPs at various concentrations and subjected to cell proliferation tests (MTT), apoptosis assays and transmission electron image analysis. Viability and apoptotic events were time and dose dependent. These in vitro tests showed that at the intermediate concentration tested there is no significant toxicity, as confirmed by transmission electron microscopy. For this reason this MNPs concentration was chosen for the subsequent in vivo tests. Oral tumor induction was performed by applying the carcinogen DMBA to Syrian hamsters. Animals were then treated by magnetohyperthermia using MNPs. No signs of general clinical symptoms of toxicity or abnormal behavioral reactions were observed. However, animals treated with MNPs and exposed to the alternating magnetic field in the hyperthermia procedure exhibited a significant and time dependent cancer regression, as confirmed by histopathological analyses and immunohistochemistry. Actually, in quantitative terms of the magnetotherapy efficacy involving these polyphosphate-coated MNPs, 100% recovery (12/12) was observed in the oral cancer tumor bearing Syrian hamsters seven days after the treatment with the magnetohyperthermia procedure. Data supports the suggestion that the MNPs-mediated hyperthermia represents a promising strategy for the treatment of oral cancer.in patients with malignancies in the mouth region. Unlike many other oncology subspecialties, however, the rates of recurrence and overall survival have improved only modestly over the past decades, despite the continuous search for new therapeutic techniques [6,7]. Thus, it is expected that the identification and use of specific agents will lead to individualized treatments, which in turn will lead to significantly better results as manifested by more cures and better quality of life by decreasing toxicity [8].Over the last years, maghemite nanoparticles (MNPs) have appeared as a well-established technology and an important field of research in superparamagnetism of colloidal materials, characteristic which allows them to be guided with an external magnetic field [9]. Recently, MNPs have been investigated for therapeutic purposes such as hyperthermic treatment [10,11]. Magnetohyperthermia (MHT) represents a novel and promising therapy for cancer treatment [11,12], being capable of promoting the specific lysis of tumor cells, thereby improving patient outcomes while minimizing the subsequent toxicity effects [13]. The Citation: Candido NM, Calmon MF, Taboga SR, Bonilha JL, dos Santos MC, et al. (2014) High Efficacy in Hyperthermia-associated with Polyphosphate

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Section: Magnetic Nanoparticle Preparation and Functionalizationmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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“…Modulation of the citrate surface-grafting onto cobalt ferrite nanoparticles was successfully achieved by varying the pH of the solution the bare particles were treated with [77]. Meso-2,3-dimercaptosuccinic acid (DMSA) has been used for surface-functionalization of bare maghemite nanoparticles at increasing surface-grafting values, revealing a rich scenario of interparticle and intra-particle linking via disulfide bridges resulting from the oxidation of neighboring thiol groups [78]. The biodistribution and biocompatibility of DMSA-coated maghemite nanoparticles have been recently tested in non-human primates [79].…”

Section: Nanoparticle-based Hyperthermiamentioning

confidence: 99%

Remote Hyperthermia, Drug Delivery and Thermometry: The Multifunctional Platform Provided by Nanoparticles

Qi¹,

Morais

2014

J Nanomed Nanotechno

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The huge demand for biocompatible, robust, accurate and noninvasive technology to assess the temperature of a biological targeted site for monitoring the hyperthermia effect brings the topic of remote thermometry to a very high level of interest. There are already promising research directions to fulfil such demand in the short term and a review of the achievements in this issue is certainly worth. This report offers an overview of the research regarding the most promising nanothermometers nowadays, with emphasis on those addressed to remote operation while using optical or magnetic responses of nanosized materials as the thermometric property. More specifically the optical emission intensity, optical emission peak shift and optical emission lifetime will be covered as far as the optical-based nanothermometers are concerned. Additionally, for the magnetic-based nanothermometers the magnetization or the magnetic susceptibility are the thermometric properties covered in this review. Furthermore, the review includes the hyperthermia effect based on nanosized metallic or magnetic particles plus a couple of thermally-responsive polymeric drug delivery systems, aiming to provide an integrated view of the multipurpose platform offered by actuating-sensing nanoparticles. As far as the regulatory system is concerned the availability of noninvasive thermometry incorporating biocompatibility, robustness and accuracy will establish the grounds needed for the approval of the hyperthermia technology for therapeutic purposes, thus allowing the market of this technological option on a global scale.hyperthermia and thermoresponsive drug delivery systems for clinical use.Although nanothermometry is at its infancy considerable progress has been made recently in regard to its use for remote assessing the temperature at the site the nanomaterials are incorporated to. Nanoprobes allowing remote temperature-sensing using optical or magnetic properties are among the most promising directions nowadays. Most of the optical-based nanothermometers use the light-emitting intensity [15][16][17][18][19][20][21][22][23], light-emitting peak shift [24][25][26], or lifetime decay of a suitable optical band as the thermometric property [27][28][29][30]. As far as the clinical use is concerned the drawbacks of the actual optical-based nanothermometers rely on biocompatibility (usual semiconductor-based core nanoprobes or dye-based shell moieties are toxic), robustness (typical bleaching of organic-based shell moieties) or temperature accuracy (no better than 0.3°C nowadays), or even combination of these factors. Moreover, due to limitations imposed by living tissues on the optical penetration of light (optical therapeutic window in the 700-1100 nm wavelength range), noninvasive clinical use of optical excitation and signal pickup is a huge challenge, not yet solved, except for very special therapeutic applications, as for instance the photodynamic therapy for treatment (heating monitoring) of skin cancer and follow up [31]. Alternatively, ...

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“…Actually, the S-S cross-linkages are alleged to play a key role in the stabilization of the built in surface molecular layer. 40 Data obtained by Raman also allow the observation of a NHS-linker effectively attached at the surface of the MNPs suspended within the MF sample, because the shoulder at 1625 cm -1 and weak features peaking is assigned to the C=O stretching of a secondary amide. Thus, the presence of this peak is claimed to be due to the vibration of a carbonyl group formed after conjugation of the anti-CEA to the DMSA linked onto the MNPs.…”

Section: Primary Antibodymentioning

confidence: 99%

Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

Paz¹,

Santos²,

Santos³

et al. 2012

IJN

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Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid) and surface-functionalized with anticarcinoembryonic antigen (anti-CEA) and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC) cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF) sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T) and a low-CEA expressing cell line (HCT116). Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter), dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm) and the zeta-potential (-38 mV) of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS) was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm -1 , absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116) confirm that the as-prepared MF-anti-CEA is highly specific for CEA-expressing cells. Finally, transmission electron microscopy analyses show that the association with anti-CEA seems to increase the number of LS174T cells with internalized maghemite nanoparticles, whereas no such increase seems to occur in the HCT116 cell line. In conclusion, the MF-anti-CEA sample is a biocompatible device that can specifically target CEA, suggesting its potential use as a theragnostic tool for CEA-expressing tumors, micrometastasis, and cancer-circulating cells.

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Spectroscopic Study of Maghemite Nanoparticles Surface-Grafted with DMSA

Cited by 37 publications

References 48 publications

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Remote Hyperthermia, Drug Delivery and Thermometry: The Multifunctional Platform Provided by Nanoparticles

Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

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