Spectroscopic studies on the interaction of alpha‐eleostearic acid with calf thymus DNA

Abstract

Interaction between alpha‐eleostearic acid (α‐ESA) and calf thymus DNA in Tris‐HCl buffer (pH = 7.4) using neutral red (NR) dye as a spectral probe was investigated using UV–Vis absorption and fluorescence spectroscopy. Spectral data matrix of the complexed reaction between α‐ESA and NR with DNA was processed with an alternative least‐squares (ALS) algorithm, the obtained concentration profiles and the corresponding pure spectra for species (NR, DNA–NR, and DNA–NR–ESA) demonstrated three kinds of reactions might occur in the system. The major groove binding between α‐ESA and DNA was further validated using circular dichroism, viscosity, DNA melting, and ionic strength effect measurements. Moreover, the calculated values of thermodynamic parameters, such as enthalpy (ΔHθ, −22.04 kJ/mol) and entropy change (ΔSθ, 91.52 J K−1 mol−1), suggested binding between α‐ESA and DNA was mainly driven by hydrophobic interactions and hydrogen bonds without electrostatic force.

Multi-Spectroscopic and Molecular Simulation Approaches to Characterize the Intercalation Binding of 1-Naphthaleneacetic Acid With Calf Thymus DNA

“…At the same time, DNA is also the primary target of many exogenous small molecules, such as drugs, fatty acids, and metal ions complexes, etc. (Qais et al, 2017 ; Liu et al, 2019 ; Zianna et al, 2019 ). However, the covalent or non-covalent binding between toxic molecules and DNA affects DNA structure, and can even affect its transcription and translation (Roy et al, 2015 ).…”