Spectroscopic studies on the interaction of fluorescein and safranine T in PC liposomes

Bozkurt, Ebru; Bayraktutan, Tuğba; Acar, Murat; Toprak, Mahmut

doi:10.1016/j.saa.2012.09.082

Cited by 22 publications

(9 citation statements)

References 35 publications

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“…Generally speaking, all the static quenching, dynamic quenching, as well as IFE can result in the fluorescence quench phenomenon. Among these different mechanisms, static quenching leads to the intense interaction and formation of a ground-state complex between the fluorophore and quencher, which generally changes the absorption spectra . In this case, we have investigated the interaction of PPDox titrated with fluorescein by qualitative isothermal titration calorimetry experiments .…”

Section: Resultsmentioning

confidence: 99%

“…Among these different mechanisms, static quenching leads to the intense interaction and formation of a ground-state complex between the fluorophore and quencher, which generally changes the absorption spectra. 37 In this case, we have investigated the interaction of PPDox titrated with fluorescein by qualitative isothermal titration calorimetry experiments. 38 Thereinto, the PPDox stock solution (1 mM) was freshly prepared by mixing PPD (1 mM), H 2 O 2 (2 mM), and HRP (20 ng/mL) in the phosphate buffer (20 mM, pH 7.0) and incubating for 30 min at room temperature.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay

et al. 2018

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Being an important model peroxidase, horseradish peroxidase (HRP) has been thoroughly understood, and the detection of HRP is not only directly related to peroxidasetriggered catalytic process, but also linked to the development of HRP-based enzyme-linked immunosorbent assay (ELISA). Herein, we have reported an unconventional fluorescent sensor for convenient assay of HRP activity based on the HRPcatalyzed specific conversion of p-phenylenediamine (PPD) into chromogenic PPDox with H 2 O 2 as the oxidizing agent, accompanied by the fluorescence quenching effect on fluorescein. By combining UV−vis absorption spectrum, isothermal titration calorimetry, and fluorescence lifetime analysis, we have confirmed the inner filter effect as a main quenching mechanism in our proposed fluorescent assay. According to the intrinsic sensitivity of fluorescent sensor and high selectivity, our PPD/fluorescein-based sensing system can be utilized for real-time monitoring of the HRP activity in real biological samples. Furthermore, the unambiguous response mechanism and excellent sensing performance encourage us to extend such HRP assay into the HRP-based fluorescent ELISA, which has a broad prospect of application in fluorescent diagnosis of hepatocellular carcinoma (HCC) by sensing alphafetoprotein, the well-known serologic HCC marker.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay

et al. 2018

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“…The dynamic quenching rate is affected by the rate of collision and increases with increasing temperature because higher temperatures increase the mobility of the quencher during diffusion. The opposite effect is seen for static quenching because the stability of the ground‐state complex diminishes with increasing temperature . In addition, with static quenching, the complex form changes the absorption spectra of the fluorophore, whereas with dynamic quenching it does not .…”

Section: Resultsmentioning

confidence: 97%

The investigation of the interaction between orientin and bovine serum albumin by spectroscopic analysis

Toprak

Arık

2013

Luminescence

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In this paper, the interaction between orientin and bovine serum albumin (BSA) was examined using fluorescence and absorbance spectroscopy. The analysis of the quenching mechanism was done using Stern-Volmer plots which exhibit upward (positive) deviation. A linear response to orientin was shown in the concentration range between 3 and 50 μM. The experimental results showed the presence of a static quenching process between orientin and BSA. The thermodynamic parameters ΔH, ΔS and ΔG were also calculated and suggested that the hydrophobic and electrostatic interactions played an important role in the interaction between orientin and BSA. Furthermore, the distances between BSA and orientin were determined according to Förster non-radiation energy transfer theory. In addition, the results of the synchronous fluorescence obtained indicated that the binding of orientin with BSA could affect conformation in BSA.

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“…It reveals that the energy transfer process which in turn depends on citric acid concentration. Samples with appropriate citric acid addition yields lesser defects, which may cause lesser non-radiation decay [31]. The excessive or insufficient citric addition generates more defective luminance centers, the non- radiation decay occurs when the relaxed-excited-state may return to the ground state through the centers mentioned above.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of photosynthetic action spectrum for 660 nm-featured phase-pure (Ba,Sr)3MgSi2O8: Eu2+, Mn2+ phosphor by spray-combustion synthesis

Sun

Mao

et al. 2015

J Mater Sci: Mater Electron

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Dual band emissions positioned at both 660 and 430 nm of (Ba, Sr) 3 MgSi 2 O 8 : Eu 2? , Mn 2? phosphor was achieved via spray-combustion synthesis procedure. At a specific limited solid solubility composition Ba 1.3 Sr 1.7 MgSi 2 O 8 , the secondary green-emitting phases of (Ba, Sr) 2 SiO 4 or (Ba, Sr)MgSiO 4 were effectively suppressed to give out the desired 660 nm broad emission. An enhancement of photoluminescence (PL) intensity for 660 nm red emission originated from Mn transition was observed in combustion synthesis samples, indicating an intrinsic promotion of energy transfer from Eu 2? to Mn 2? in (Ba, Sr) 3 MgSi 2 O 8 host. This PL enhancement to 660 nm emission is probably ascribed to an effective reduction of Mn ion in the combustion process with the aid of decomposed reductive radicals formed in situ upon fuel ignition. The crystallized particle size varies in the range from 5 to 20 lm depending on process parameters. A near ultraviolet LED illumination prototype packaged with as-prepared phosphor exhibits to be violet in color. This combustion synthesis process provides an effective means for PL enhancement of phase-pure (Ba,Sr) 3 MgSi 2 O 8 : Eu 2? , Mn 2? phosphors to construct the photosynthetic action spectrum.

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Spectroscopic studies on the interaction of fluorescein and safranine T in PC liposomes

Cited by 22 publications

References 35 publications

Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay

Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay

The investigation of the interaction between orientin and bovine serum albumin by spectroscopic analysis

Enhancement of photosynthetic action spectrum for 660 nm-featured phase-pure (Ba,Sr)3MgSi2O8: Eu2+, Mn2+ phosphor by spray-combustion synthesis

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