Spectroscopic Studies on the Binding of a New Quinolone Antibacterial Agent: Sinafloxacin to DNA

Yan, Fei; L, Gao; Fan, Guorong; Wu, Yutian

doi:10.2116/analsci.25.1333

Cited by 64 publications

(20 citation statements)

References 29 publications

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“…In the presence of DNA, the cyclic voltammograms of EFZ exhibited positive shift in the anodic peak potential followed by a decrease in the peak current, indicating the interaction that exists between EFZ and DNA. The decreases in peak current can be explained in terms of slow diffusion of EFZ bound to the large DNA molecules , and also support the changes found in CV experiments. Hence it can be concluded that the EFZ molecule binds to DNA via intercalation, with insertion of the EFZ molecule between the base pairs of the DNA duplex strand.…”

Section: Resultssupporting

confidence: 82%

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

et al. 2015

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Interactions between efavirenz (EFZ) with calf thymus DNA (CT-DNA) were investigated in vitro under stimulated physiological conditions using multispectroscopic techniques, cyclic voltammetry viscosity measurement, and gel electrophoresis. Methylene blue and acridine orange dyes were used as spectral probes by fluorescence spectroscopy. Hypochromicity was observed in ultra-violet (UV) absorption band of EFZ. Considerable fluorescence enhancement of EFZ was observed in the presence of increasing amounts of DNA solution and the binding constants (Kf ) and corresponding numbers of binding sites (n) were calculated at different temperatures. Thermodynamic parameters including enthalpy change (ΔH) and entropy change (ΔS) were calculated to be -304.78 kJ mol(-1) and -924.52 J mol(-1) K(-1) according to the van 't Hoff equation, which indicated that reaction is predominantly enthalpically driven. In addition, UV/vis absorption titration of DNA bases confirmed that EFZ interacted with guanine and cytosine preferentially. Gel electrophoresis of DNA with EFZ demonstrated that EFZ also has the ability to cleave supercoiled plasmid DNA. Circular dichroism study showed stabilization of the right-handed B form of CT-DNA. All results suggest that EFZ interacts with CT-DNA via an intercalative mode of binding.

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Section: Resultssupporting

confidence: 82%

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

et al. 2015

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“…The voltammogram exhibited remarkable shifts in the anodic and cathodic peak potentials along with a decrease in both peak currents, indicating the a strong interaction had occurred between metal complexes and HS–DNA …”

Section: Resultsmentioning

confidence: 99%

Cu(II) and Ni(II) Complexes of Anthracene‐Affixed Schiff Base: A Conflict between Covalent and Stacking Interactions with DNA Bases

2017

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Four new mononuclear complexes [Cu(L1)Cl 2 ] (1), [Ni(L1)Cl 2 ] (2), [Cu(L2)Cl 2 ] (3) and [Ni(L2)Cl 2 ] (4), where L1 (1-(anthracen-9-yl)-N-(1H-benzo[d]imidazole-2-yl)methanimine) and L2 (1-(anthracen-9-yl)-N-(benzo[d]thiazol-2yl)methanimine) have been synthesized and characterized by various spectral and electro analytical techniques. The molecular structure of the ligands L1 and L2 were determined by the single crystal XRD method. The absorption and emission spectral approaches revealed that these complexes may partially be stacking in between the DNA bases. In contrast, the possibility of their covalent interaction was evaluated through the cyclic voltammetric techniques. All these obtained results revealed that the complexes 1-4 could interact with HS-DNA via covalent/partial intercalation modes and the quenching mechanism of BSA by all these complexes are through static mode. In addition, the in vitro cytotoxicity of the complexes on human gastric cancer cell lines have been observed by MTT and the fluorescence-based morphological assessment obtained using Hoechst 33342 staining reveals that these complexes induces apoptosis against AGS cancer cells, among them best result was obtained for complex 3 (8.9 mM).

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“…The G factor is defined as 25 G = I HV / I HH (2) where the intensities I HV and I HH refer to the vertical and horizontal positions of the emission polarizer, with the excitation polarizer being horizontal. The experiments were carried out at an ambient temperature of 298 K.…”

Section: Steady State Spectral Measurementsmentioning

confidence: 99%

Binding of an anionic fluorescent probe with calf thymus DNA and effect of salt on the probe–DNA binding: a spectroscopic and molecular docking investigation

et al. 2014

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Spectroscopic Studies on the Binding of a New Quinolone Antibacterial Agent: Sinafloxacin to DNA

Cited by 64 publications

References 29 publications

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

Cu(II) and Ni(II) Complexes of Anthracene‐Affixed Schiff Base: A Conflict between Covalent and Stacking Interactions with DNA Bases

Binding of an anionic fluorescent probe with calf thymus DNA and effect of salt on the probe–DNA binding: a spectroscopic and molecular docking investigation

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