Spectroscopic Studies on the Binding of Bacteriophage Mequindox with Bovine Serum Albumin

Abstract

Fluorescence spectra and UV-Vis absorption spectra have been used to study the binding of bacteriophage mequindox (MEQ) with bovine serum albumin (BSA), which performed a dynamic quenching process. The quenching constants and thermodynamic parameters at different temperatures were calculated. The binding was primarily driven by entropy, and hydrophobic forces also played a significant role. The distance between BSA and MEQ was estimated to be 4.5 nm based on the theory of Förster's non-radioactive energy transfer. Furthermore, synchronous fluorescence spectra and 3-dimensional fluorescence spectra were used to figure out the configuration of BSA in the presence or absence of MEQ, which indicated that it was basically the same.

Study on the interaction between promethazine hydrochloride and bovine serum albumin by fluorescence spectroscopy

“…The maximum emission wavelengths of tyrosine residues shifted towards short wavelength upon addition of PMT, which indicated that the polarities around tyrosine residues were decreased and the hydrophobicity was increased and the amino acid residues were less exposed to the solvent with the addition of PMT [40]. Three-dimensional fluorescence spectroscopy is a new analytical technique which is applied to investigate the conformational changes of proteins in recent years [41,42]. The excitation wavelength, the emission wavelength and the fluorescence intensity can be used as the axes making the investigation of the characteristic conformational changes of proteins be more scientific and credible [43,44].…”

Confined and in-situ zeolite synthesis: A novel strategy for defect reparation over dense Pd membranes for hydrogen separation

“…According to the supplier, the average pore diameter of the γ-Al 2 O 3 is about 0.2 μm. Ultra-thin Pd membranes were deposited on γ-Al 2 O 3 surface by a modified electroless plating method [38]. Four…”

Manufacture, Applications, and Opportunities of Thin Film Palladium-Silver (PdAg) Alloys: A Review

“…10,20 Pd and Ag both crystallise in the face-centred cubic (fcc) lattice with lattice constants of 389.07 and 405.83 Ǻ respectively. [20][21][22] The infinite miscibility of the two elements means that the expansion of the Pd lattice as Ag is doped into the structure is linear. [20][21][22] The resistivity and thus electrical conductivity of Pd-Ag alloys, vary extensively with both temperature and composition.…”

“…[20][21][22] The infinite miscibility of the two elements means that the expansion of the Pd lattice as Ag is doped into the structure is linear. [20][21][22] The resistivity and thus electrical conductivity of Pd-Ag alloys, vary extensively with both temperature and composition. 13 The room temperature variation with alloy composition is outlined in Figure 1.…”

“…20 Certain applications may use materials prepared a single manufacturing technique, as the properties essential end use of the material are closely influenced by the preparative route. For example, PdAg thin films for sensor applications are commonly prepared using CVD or PVD, 36,[65][66][67][68][69][70][71][72][73] while those for use in gas separation may use electroless deposition 14,22,28,31,35,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89] or mechanical methods 14,33,85,[92][93][94] though this is not exclusive.…”