Spectroscopic Studies of Interaction of Protein with Cerium Oxide Nanoparticles

Abraham, S. Daniel

doi:10.14233/ajchem.2018.21207

Cited by 3 publications

(3 citation statements)

References 26 publications

(35 reference statements)

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“…20 In the view of strong interest to the effects of TRP interaction with NP, surprisingly little has been made on investigation of its molecular mechanisms. Except some optical spectroscopy studies 21 and the work of covalently bound Tryptophan, 22 we were, to the best of our knowledge, not able to nd any structural and mechanistic characterization of TRP-NP interactions. In the present study, we report NMR characterization of TRP interaction with surface capped ceria and titania, and bare tungsten oxide (as POM model) and an X-ray single crystal study and theoretical investigation of the latter complex with TRP.…”

Section: Introductionmentioning

confidence: 82%

Molecular mechanisms in metal oxide nanoparticle Tryptophan interactions

Kessler

Nefedova

Svensson

et al. 2023

Preprint

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One of the crucial metabolic processes for both plant and animal kingdoms is oxidation of amino acid tryptophan (TRP) that regulates the plant growth and controls hunger and sleeping patterns in animals. Here, we report revolutionary insights into how this process can be crucially effected by interactions with metal oxide nanoparticles (NP), creating a toolbox for a plethora of important biomedical and agricultural applications. Molecular mechanisms in TRP-NP interactions were revealed by NMR and optical spectroscopy for ceria and titania, and by X-ray single crystal and a computational study of a model TRP-polyoxometalate complexes. Nanozyme activity, involving concerted proton and electron transfer to the NP surface for oxides with high oxidative potential, CeO2 or WO3, converted TRP into a tricyclic organic acid resembling natural plant hormones, auxins. TiO2, a much poorer oxidant, was strongly binding TRP without concurrent oxidation in the dark, but oxidized it non-specifically via release of reactive oxygen species (ROS) in daylight.

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Section: Introductionmentioning

confidence: 82%

Molecular mechanisms in metal oxide nanoparticle Tryptophan interactions

Kessler

Nefedova

Svensson

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…20 In view of the strong interest to the effects of the TRP interaction with NPs, surprisingly little effort has been made on the investigation of its molecular mechanisms. Except some optical spectroscopy studies 21 and the work of covalently bound tryptophan, 22 we were, to the best of our knowledge, not able to find any structural and mechanistic characterization of TRP−NP interactions. In the present study, we report the NMR characterization of the TRP interaction with bare and capped ceria and titania surfaces and the bare tungsten oxide surface (in the form of a POM model, phosphotungstic acid) and an X-ray single-crystal study and theoretical investigation of the latter complex with TRP, which provided direct evidence for a new mechanism of the direct (not via intermediate reactive oxygen species, ROS) oxidation of the amino acid via simultaneous electron and proton transfer.…”

Section: ■ Introductionmentioning

confidence: 83%

Molecular Mechanisms in Metal Oxide Nanoparticle–Tryptophan Interactions

Nefedova,

Svensson,

Vanetsev

et al. 2024

Inorg. Chem.

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One of the crucial metabolic processes for both plant and animal kingdoms is the oxidation of the amino acid tryptophan (TRP) that regulates plant growth and controls hunger and sleeping patterns in animals. Here, we report revolutionary insights into how this process can be crucially affected by interactions with metal oxide nanoparticles (NPs), creating a toolbox for a plethora of important biomedical and agricultural applications. Molecular mechanisms in TRP− NP interactions were revealed by NMR and optical spectroscopy for ceria and titania and by X-ray single-crystal study and a computational study of model TRP−polyoxometalate complexes, which permitted the visualization of the oxidation mechanism at an atomic level. Nanozyme activity, involving concerted proton and electron transfer to the NP surface for oxides with a high oxidative potential, like CeO 2 or WO 3 , converted TRP in the first step into a tricyclic organic acid belonging to the family of natural plant hormones, auxins. TiO 2 , a much poorer oxidant, was strongly binding TRP without concurrent oxidation in the dark but oxidized it nonspecifically via the release of reactive oxygen species (ROS) in daylight.

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“…CeO 2 nanoparticles triggered the transition of hen egg white lysozyme secondary structure from α-helix to β-sheet and induced the hydrophobic region of lysozyme to become exposed to the solvent [190]. They also interact with bovine serum albumin with minor conformational changes in the biomolecular structure [191]. MONPs all interacted with the phosphate backbone of DNA, but exhibited different DNA adsorption affinity [192,193].…”

Section: Fluorescence Detection Of Transition Metal Oxide Nanoparticlesmentioning

confidence: 99%

Current State of Laser-Induced Fluorescence Spectroscopy for Designing Biochemical Sensors

Taylor

Lai

2021

Chemosensors

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Laser-induced fluorescence (LIF) has been a valuable analytical technique since the 1970s that has only been made more useful through advances in other scientific fields such as biochemistry. Moreover, advances in laser and detector technology have seen a decrease in LIF detector costs and an increase in their ease of use. These changes have allowed for LIF technology to be widely adopted for various sensor designs in combination with advanced instruments. With advances in biochemistry necessitating the detection of complex metabolites, labelling with fluorescent chemical reagents may be necessary to improve detection sensitivity. Furthermore, advances made in fluorescent labeling technologies have allowed for the use of LIF in the detection of nanoparticles as well as for imaging techniques using nanoparticles as signal amplifiers. This technology has become invaluable in the detection of environmental pollutants, monitoring of biological metabolites, biological imaging, and cancer diagnosis, making it one of the most valuable analytical science techniques currently available.

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Spectroscopic Studies of Interaction of Protein with Cerium Oxide Nanoparticles

Cited by 3 publications

References 26 publications

Molecular mechanisms in metal oxide nanoparticle Tryptophan interactions

Molecular mechanisms in metal oxide nanoparticle Tryptophan interactions

Molecular Mechanisms in Metal Oxide Nanoparticle–Tryptophan Interactions

Current State of Laser-Induced Fluorescence Spectroscopy for Designing Biochemical Sensors

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