1994
DOI: 10.1007/bf01881886
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Spectroscopic properties of the photoproducts of pyridoxal-5′-P irradiation: Catalytic site recognition of ribonuclease A

Abstract: Photoproducts of pyridoxal-5'-P, i.e., 4-pyridoxic-5'-P and bis-pyridoxal-5'-P, have been studied by spectroscopic methods. The spectroscopic properties of bis-pyridoxal-5'-P (bis-PLP) resemble those of pyridoxal-5'-P (PLP) under similar experimental conditions. The coupling of methylen hydrogens to the phosphorus atom has been shown by NMR spectroscopy. The singlet in the(31)P-NMR spectra and the triplet in(1)H-undecoupled experiments confirm the presence of the phosphate group in the 5' position of the struc… Show more

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