Spectroscopic properties of chloroplast grana membranes and of the core of Photosystem II

Dorssen, R.J. van; Plijter, Johan J.; Dekker, Jan; Ouden, A. den; Amesz, J.; Gorkom, Hans J. van

doi:10.1016/0005-2728(87)90014-4

Cited by 99 publications

(64 citation statements)

References 29 publications

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“…The reduced intensity of the 683 nm band in vir-m 29 (Table II) is consistent with the partial loss of PSII activity. Also consistent with this assignment is the presence of a 683 nm component (to = 8 nm) in PSII core particles (28). This band represents 8-10 of the 45 chlorophyll molecules in the complex, and consists of more than one component, including the reaction centre pigment P680 and pheophytin a.…”

Section: Viridls-k 2jsupporting

confidence: 69%

“…This can be done by isolating the chlorophyll-proteins (3,14,24,28), although the use of detergents during the isolation is a possible source of artefact. Alternatively, specific chlorophyll-proteins can be removed through mutation, and the absorption spectra of the resulting mutants recorded in vivo.…”

Section: Viridls-k 2jmentioning

confidence: 99%

“…Analysis of the absorption spectra of isolated chlorophyll-proteins (3,4,14,15,24,28), and of Chlamydomonas mutants (2,8) by Gaussian deconvolution and their fourth derivative, has shown that certain long wavelength absorbing forms of chlorophyll a are specifically associated with PSII (Ca683) and others with PSI (Ca691). The present paper reports the low temperature absorption spectra from a number of barley nuclear gene mutants lacking one or more specific chlorophyll-proteins.…”

Section: Introductionmentioning

confidence: 99%

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Low temperature absorption spectroscopy of barley mutants. Guassian deconvolution and fourth derivative analysis

Simpson

1988

Carlsberg Res. Commun.

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Keywords: Chlorophyll-proteins, photosystem I/II, thylakoids "Une absorption spectra of wild type barley and a number of nuclear gene mutants have been recorded at 77 K, and analysed by taking the fourth derivative and Gaussian deconvolution in the region 600-725 nm. Seven Gaussian curves could be fitted, corresponding to the Qy transitions of at least 7 different chlorophyll species, absorbing at 640.5 and 649.9 nm (chlorophyll b), 660.7, 668.9, 676.6, 682.9 and 688.9 nm (chlorophyll a). The first 2 components were missing from the chlorophyll b-less mutant clo-f228~176 revealing a minor chlorophyll a species at 653 nm. The same 7 wild type components could also be fitted to the low temperature absorption spectra of the mutants vir-m 29and vir-k 2J. The loss of photosystem II from the mutant vir-n~ was associated with the specific loss of the 683 nm component, corresponding to about 30-40 molecules of chlorophyll a per 500 molecules of chlorophyll. Similarly, the absence of photosystem I from the mutant vir-zb 6J was correlated with the loss of 30 molecules of chlorophyll a absorbing at 689 nm. These specific differences were confirmed by the absorption difference spectra and by fourth derivative spectroscopy. The validity of the solutions provided by Gaussian deconvolution were evaluated by comparing their fourth derivative with that of the original spectra.

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Section: Viridls-k 2jsupporting

confidence: 69%

Section: Viridls-k 2jmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low temperature absorption spectroscopy of barley mutants. Guassian deconvolution and fourth derivative analysis

Simpson

1988

Carlsberg Res. Commun.

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“…Simple spectrophotometric and photophysical criteria have been developed to assess the integrity and functional competence of the preparations, with low-temperature fluorescence being especially sensitive. Note that the fluorescence spectra in Figure 3 do not display maxima at 695 nm (see also Ref. 2) previously assigned to the PSII RC (3,23) and observed in O,-evolving core complexes (31). Figure 3 also suggests that Triton X-100 is the cause of the instability.…”

Section: Methodsmentioning

confidence: 56%

Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center

Seibert¹,

Picorel²,

Rubin³

et al. 1988

Plant Physiol.

146

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Photosystem II reaction center (RC) preparations isolated from spinach (Spinacea oleracea) by the Nanba-Satoh procedure (O Nanba, K Satoh 1987 Proc Nati Acad Sci USA 84: 109-112) are quite labile, even at 4°C in the dark. Simple spectroscopic criteria were developed to characterize the native state of the material. Degradation of the RC results in (a) blueshifting of the red-most absorption maximum, (b) a shift of the 77 K fluorescence maximum from -682 nm to -670 nm, and (c) a shift of fluorescence lifetime components from 1.3-4 nanoseconds and >25 nanoseconds to -6-7 nanoseconds. Fluorescence properties at 77 K seem to be a more sensitive spectral indicator of the integrity of the material. The >25 nanosecond lifetime component is assigned to P680+ Pheophytinrecombination luminescence, which suggests a correlation between the observed spectral shifts and the photochemical competence of the preparation. Substitution of lauryl maltoside for Triton X-100 immediately after RC isolation stabilizes the RCs and suggests that Triton may be responsible for the instability.RC4 complexes found in photosynthetic membranes contain specialized pigment components that carry out the primary photochemical processes of photosynthesis. These light reactions provide the chemical potential to drive dark electron transport that leads to the storage of energy required by the organism. The first RC fractions were isolated from the photosynthetic bacteria Rhodobacter sphaeroides R-26 (25) and Rhodospirillum rubrum S1 (14) in the late 1960s. This accomplishment has led to a great deal of progress in understanding of the function of the RC. Perhaps the most far-reaching effect of this work, however, culminated in the crystallization of the Rhodopseudomonas viridis RC, which was the first integral membrane protein complex to be crystallized (22 (8,10,18) have suggested that the Dl and D2 proteins constitute the reaction center of PSII. The first experimental evidence suggesting that this might be true was the observation that the Dl protein functions or affects function on both sides of the PSII RC (21). More recently, Nanba and Satoh (24) reported the isolation of a PSII complex composed of Dl, D2, and Cyt b-559 which they identified as the RC. Danielius et al. (9) confirmed this identification by showing that the material could transfer an electron from P680 to endogenous pheophytin. In this paper we confirm the isolation of the Nanba-Satoh preparation and report on several spectral properties that appear to relate to the stability of the isolated PSII reaction center.MATERIALS AND METHODS PSII RC fractions were prepared from market spinach (Spinacia oleracea) by the procedure of Nanba and Satoh (24). All steps were carried out at 4°C in the dark. Appressed thylakoid membrane fragments devoid of PSI (13) were obtained from market spinach using Triton X-100 (17). PSII membranes were pelleted and then resuspended in 50 mm Tris-HCl (pH 7.2). The material (30 ml of 1 mg Chl/ml final concentration) was then stirred for 1 h with 4%...

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“…Fluorescence spectra at 77 K are often used to monitor ultrastructural changes in thylakoid membranes as a response to environmental condition variations. Thus F 735 /F 685 and F 735 /F 695 ratios can be used as a probe for the amount of antenna Chls connected to each photosystem (van Dorssen et al, 1987;Alfonso et al, 1994). The antenna Chls of photosystem I emit maximum fluorescence at 735 nm whereas those of LHCII surrounding PSII emit at 680 nm.…”

Section: Effect Of Cu Treatments On Soybean Plantsmentioning

confidence: 99%

Foliar and root Cu supply affect differently Fe- and Zn-uptake and photosynthetic activity in soybean plants

Bernal

Cases

Picorel

et al. 2007

Environmental and Experimental Botany

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Spectroscopic properties of chloroplast grana membranes and of the core of Photosystem II

Cited by 99 publications

References 29 publications

Low temperature absorption spectroscopy of barley mutants. Guassian deconvolution and fourth derivative analysis

Low temperature absorption spectroscopy of barley mutants. Guassian deconvolution and fourth derivative analysis

Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center

Foliar and root Cu supply affect differently Fe- and Zn-uptake and photosynthetic activity in soybean plants

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