Spectroscopic Investigation on the Interaction of Pyrimidine Derivative, 2-Amino-6-hydroxy-4-(3,4-dimethoxyphenyl)-pyrimidine-5-carbonitrile with Human Serum Albumin: Mechanistic and Conformational Study

Suryawanshi, Vishwas D.; Anbhule, Prashant V.; Gore, Anil H.; Patil, Shivajirao R.; Kolekar, Govind B.

doi:10.1021/ie202005c

Cited by 61 publications

(22 citation statements)

References 50 publications

(70 reference statements)

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“…occurred via the π-π stacking between aromatic rings of compound 5d and Trp, Tyr and Phe residues possessed conjugated π-electrons and located in the binding cavity of HSA [34]. [35,36].…”

Section: Uv-visible Absorption Spectral Studymentioning

confidence: 99%

Design, synthesis and biological evaluation of berberine-benzimidazole hybrids as new type of potentially DNA-targeting antimicrobial agents

Jeyakkumar

Zhang

Avula

et al. 2016

European Journal of Medicinal Chemistry

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Section: Uv-visible Absorption Spectral Studymentioning

confidence: 99%

Design, synthesis and biological evaluation of berberine-benzimidazole hybrids as new type of potentially DNA-targeting antimicrobial agents

Jeyakkumar

Zhang

Avula

et al. 2016

European Journal of Medicinal Chemistry

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“…where F 0 and F are the fluorescence intensity of donor (pyrene) in absence and presence of acceptor (perylene), respectively [22,23]. The efficiency of energy transfer depends upon critical transfer distance (R 0 ) between pyrene and perylene is calculated by using the Förster relation [10]:…”

Section: Energy Transfer Efficiencymentioning

confidence: 99%

Fluorescence enhancement effect in pyrene and perylene doped nanoporous polystyrene films: Mechanistic and morphology

Desai

Gupta

Kolekar

et al. 2013

Physica Status Solidi (a)

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Luminescent porous polystyrene (PS) films containing pyrene and various amounts of perylene were prepared in humid atmosphere by solution casting technique for use as a plastic scintillator. The fluorescence spectra of doped PS films monitored at pyrene excitation show quenching of monomer fluorescence of pyrene with simultaneous sensitization of perylene monomer fluorescence appearing towards red. The observed enhancement in fluorescence of perylene was explained by considering excitation energy transfer (EET) from pyrene to perylene molecules accommodated in the pores generated during film formation. The fluorescence quenching data do not fit into the Stern–Volmer relation, but the sensitized fluorescence is fitted by an exponential growth equation, y = 201 860 433.29 ex/0.64366 + 0.49262, with correlation coefficient R2 = 0.997. The results on absorption and excitation spectroscopy indicated that the pyrene and perylene filled in pores of the PS films exist as isolated molecules. The porosity of the films was examined by atomic force microscopy (AFM) and scanning electron microscopy (SEM), showing randomly oriented holes, and their mean size and size distribution were found to be depending upon the dopant concentration. Pyrene and perylene doped porous PS films prepared by solution casting method in humid atmosphere showed highly intense blue perylene fluorescence by fluorescence resonance energy transfer (FRET) at pyrene excitation. The SEM and AFM images revealed that nanosized pores are perylene concentration dependent, both pyrene and perylene held in the cavity of the pores in close proximal distance less than 10 nm is required to cause efficient energy transfer between them.

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“…The overlap of absorption spectrum of ID and emission spectrum of HSA is displayed in Figure . For HSA, k 2 =2/3, n=1.336 and Φ=0.118 . By using equations (6) ‐ (8), we have calculated the values of J, R o , E, and r and noticed to be 0.78 x10 −15 cm 3 L mol −1 , 2.99 nm, 0.71 and 2.81 nm respectively.…”

Section: Resultsmentioning

confidence: 99%

Interaction of Indole Derivative with Human Serum Albumin: A Combined Spectroscopic and Molecular Dynamics Study

2018

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In the present work, we have studied the interaction of synthesized indole derivative (ID) with a model protein, human serum albumin (HSA). Various spectroscopic and molecular dynamic simulation methods were employed to characterize the binding between ID and HSA. ID showed strong binding affinity towards HSA (1.86x106 M−1) and quenched the fluorescence intensity of HSA by dynamic quenching mechanism. The existence of dynamic quenching mechanism in ID‐HSA interaction was further confirmed by lifetime measurement study. ID‐HSA interaction was favored by hydrophobic forces. The binding site for ID on HSA was examined by site probe competitive experiments and molecular docking studies. The results revealed that ID was bound to HSA primarily at site I of subdomain IIA. Absorption, 3D fluorescence and circular dichroism (CD) studies provided information on the conformational and microenvironmental changes in HSA upon binding to ID.

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Spectroscopic Investigation on the Interaction of Pyrimidine Derivative, 2-Amino-6-hydroxy-4-(3,4-dimethoxyphenyl)-pyrimidine-5-carbonitrile with Human Serum Albumin: Mechanistic and Conformational Study

Cited by 61 publications

References 50 publications

Design, synthesis and biological evaluation of berberine-benzimidazole hybrids as new type of potentially DNA-targeting antimicrobial agents

Design, synthesis and biological evaluation of berberine-benzimidazole hybrids as new type of potentially DNA-targeting antimicrobial agents

Fluorescence enhancement effect in pyrene and perylene doped nanoporous polystyrene films: Mechanistic and morphology

Interaction of Indole Derivative with Human Serum Albumin: A Combined Spectroscopic and Molecular Dynamics Study

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