Spectroscopic characterization of antibodies adsorbed to aluminium adjuvants: correlation with antibody vaccine immunogenicity

Improved formulation of a recombinant ricin A-chain vaccine increases its stability and effective antigenicity

“…Chang et al [31] reported that the antigenicity of lysozyme in rabbits was independent of its adherence to aluminum adjuvant during preparation, while Romero Mendez et al [32] proposed that trapping of a nonadsorbed protein in a porous matrix of aluminum phosphate adjuvant was adequate to stimulate the immune response. On the other hand, Capelle et al [33] suggested that stronger adherence of an immunoglobulin antigen to adjuvant results in the formation of more condensed particles of greater immunogenicity. Berthold et al [34] concluded that adsorption of recombinant anthrax protective antigen to aluminum phosphate enhances the immunogenicity of lower doses of antigen.…”

Biophysical and formulation studies of theSchistosoma mansoniTSP-2 extracellular domain recombinant protein, a lead vaccine candidate antigen for intestinal schistosomiasis

“…10 A number of biophysical analyses specifically looking at the structure of model antigens bound to aluminum based hydrated gels, have revealed none or subtle effects upon the bound protein. [11][12][13][14][15][16] Even though alum adjuvantation is not expected to affect the properties of Sm-Tsp-2, future studies will be able to address and document that this indeed is the case.…”

Physical instability, aggregation and conformational changes of recombinant human bone morphogenetic protein-2 (rhBMP-2)

“…Various complementary methods, such as intrinsic and extrinsic (hydrophobic dyes Nile Red and 1,8-ANS) fluorescence spectroscopy, light scattering and transmission electron microscopy (TEM) were used to compare rhBMP-2 solutions at pH 4.5 and 6.5. These analytical techniques are well established for the characterization of chemical and physical stability of protein formulations (Pellaud et al, 1999;Lakowicz, 2004;Capelle et al, 2005;Jiskoot and Crommelin, 2005;Demeule et al, 2007b;Hawe et al, 2008).…”