Spectroscopic characterization and docking studies of ZnO nanoparticle modified with BSA

Ledesma, Ana Estela; Chemes, Doly M.; Frías, María de los Ángeles; Torres, María del Pilar Guauque

doi:10.1016/j.apsusc.2017.03.202

Cited by 40 publications

(16 citation statements)

References 42 publications

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“…Amide I and II band intensity can also provide quantitative information about adsorbed proteins [ 88 , 123 ]. Raman spectroscopy is a technique mostly applied for studying adsorption on nanoparticles [ 36 , 237 ], which was also reported to be used, with 2D-correlation analysis, for adsorption on bulk materials [ 71 ]. The evolution of the adsorbing layer can be monitored in situ by spectroscopic ellipsometry by measuring the thickness of the layer, according to the variation of the ellipsometric angles Δ and Ψ [ 86 , 208 , 209 ].…”

Section: Methods For Investigating Protein Adsorption On Titanium-mentioning

confidence: 99%

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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Titanium and its alloys, specially Ti6Al4V, are among the most employed materials in orthopedic and dental implants. Cells response and osseointegration of implant devices are strongly dependent on the body–biomaterial interface zone. This interface is mainly defined by proteins: They adsorb immediately after implantation from blood and biological fluids, forming a layer on implant surfaces. Therefore, it is of utmost importance to understand which features of biomaterials surfaces influence formation of the protein layer and how to guide it. In this paper, relevant literature of the last 15 years about protein adsorption on titanium-based materials is reviewed. How the surface characteristics affect protein adsorption is investigated, aiming to provide an as comprehensive a picture as possible of adsorption mechanisms and type of chemical bonding with the surface, as well as of the characterization techniques effectively applied to model and real implant surfaces. Surface free energy, charge, microroughness, and hydroxylation degree have been found to be the main surface parameters to affect the amount of adsorbed proteins. On the other hand, the conformation of adsorbed proteins is mainly dictated by the protein structure, surface topography at the nano-scale, and exposed functional groups. Protein adsorption on titanium surfaces still needs further clarification, in particular concerning adsorption from complex protein solutions. In addition, characterization techniques to investigate and compare the different aspects of protein adsorption on different surfaces (in terms of roughness and chemistry) shall be developed.

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Section: Methods For Investigating Protein Adsorption On Titanium-mentioning

confidence: 99%

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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“…The characterization of the binding site between the BSA protein with AG and ZnO nanoparticle was estimated by molecular docking. For ZnO-NPs docking, the structure reported by our previous studies was used [28]. Flat ZnO-NPs surfaces (15 × 25 Å) mimicking the particle curvature of a ZnO-NPs were constructed.…”

Section: Molecular Docking Of Bsa With Zno-nps and Agmentioning

confidence: 99%

Biosynthesized ZnO-NPs from Morus indica Attenuates Methylglyoxal-Induced Protein Glycation and RBC Damage: In-Vitro, In-Vivo and Molecular Docking Study

et al. 2019

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The development of advanced glycation end-products (AGEs) inhibitors is considered to have therapeutic potential in diabetic complications inhibiting the loss of the biomolecular function. In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized from aqueous leaf extract of Morus indica and were characterized by various techniques such as ultraviolet (UV)-Vis spectroscopy, Powder X-Ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). Further, the inhibition of AGEs formation after exposure to ZnO-NPs was investigated by in-vitro, in-vivo, and molecular docking studies. Biochemical and histopathological changes after exposure to ZnO-NPs were also studied in streptozotocin-induced diabetic rats. ZnO-NPs showed an absorption peak at 359 nm with a purity of 92.62% and ~6–12 nm in size, which is characteristic of nanoparticles. The images of SEM showed agglomeration of smaller ZnO-NPs and EDS authenticating that the synthesized nanoparticles were without impurities. The biosynthesized ZnO-NPs showed significant inhibition in the formation of AGEs. The particles were effective against methylglyoxal (MGO) mediated glycation of bovine serum albumin (BSA) by inhibiting the formation of AGEs, which was dose-dependent. Further, the presence of MGO resulted in complete damage of biconcave red blood corpuscles (RBCs) to an irregular shape, whereas the morphological changes were prevented when they were treated with ZnO-NPs leading to the prevention of complications caused due to glycation. The administration of ZnO-NPs (100 mg Kg−1) in streptozotocin(STZ)-induced diabetic rats reversed hyperglycemia and significantly improved hepatic enzymes level and renal functionality, also the histopathological studies revealed restoration of kidney and liver damage nearer to normal conditions. Molecular docking of BSA with ZnO-NPs confirms that masking of lysine and arginine residues is one of the possible mechanisms responsible for the potent antiglycation activity of ZnO-NPs. The findings strongly suggest scope for exploring the therapeutic potential of diabetes-related complications.

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“…This allows negatively charged proteins to form hydrogen bonds at the lattice oxygen on the nonpolar surfaces of nanorods. 22 …”

Section: Resultsmentioning

confidence: 99%

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

Liu

Dai

2021

ACS Omega

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ZnO electrospun nanofibers can act as seed fibers to fabricate multidentate barbed fibers perpendicular to the growth of the fibers using the chemical bath deposition (CBD) method. Fibers with a multirod morphology have a porous grid structure. The sample is easy to recover, and the nonpolar surface in the sample is sufficiently exposed. In the research of barbed fiber fabrication and adsorption on bovine serum albumin (BSA), the effects of different chemical bath conditions on the growth of ZnO nanorods were discussed. Barbed fibers with large slenderness ratios were obtained at a water content of 60 mL at 75 °C. Each milligram of barbed fibers can quickly adsorb about 162 μg of protein within 30 min. The adsorption activity of BSA between polar and nonpolar ZnO surfaces was also studied. The selective adsorption behavior of BSA on the nonpolar surface was revealed.

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Spectroscopic characterization and docking studies of ZnO nanoparticle modified with BSA

Cited by 40 publications

References 42 publications

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Biosynthesized ZnO-NPs from Morus indica Attenuates Methylglyoxal-Induced Protein Glycation and RBC Damage: In-Vitro, In-Vivo and Molecular Docking Study

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

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