“…Moreover, as H 3 [EB] + showed a significant positive charge, the intermolecular electrostatic attraction or hydrogen bond structure would be generated, resulting in molecule self‐aggregation, thus forming hydrophobic molecular clusters, which would precipitate and detach from the homogeneous solution system after a period of times (Figure 2b). [21] The research of Snigur and co‐workers [17] have supported our discussions above, and p K a of H 3 [EB] + in each‐order ionization based on tristimulus colourimetry were given (3.50, 4.90 and 10.10, respectively). Synthesizing above explorations and previous studies about fluorescein structures, [20, 22] we proposed the pathways of EB protonation processes and the detailed mechanisms of ISR and IDR (Figure 2d).…”