Spectroscopic and biochemical correlations during the course of human lens aging

Ranjan, Mala; Beedu, Sashidhar Rao

doi:10.1186/1471-2415-6-10

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…These findings agree with the well-known fact that human lenses become progressively more yellow during the process of aging [9,10,11,12,13,14,15,18]. …”

Section: Discussionsupporting

confidence: 82%

“…A white light source and a costly spectrometer were usually used in previous studies [9,10,11,12,13,14] while we used four different colored lights with a not so costly CCD camera. Thus, we are able to examine the spectral transmittance of the crystalline lenses using a relatively simple and reasonable method.…”

Section: Discussionmentioning

confidence: 99%

“…The spectral transmittance of human crystalline lenses has been measured [9,10,11,12,13,14], but the measurements have been made mainly on extracted lenses. To the best of our knowledge, there is only one study, by Said and Weale [15], that measured the spectral transmittance of the human lens in situ using the third and fourth Purkinje-Sanson mirror images (PSMIs).…”

Section: Introductionmentioning

confidence: 99%

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Age-Related Changes in Spectral Transmittance of the Human Crystalline Lens in situ

Sakanishi

Awano²,

Mizota³

et al. 2012

Ophthalmologica

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Aims: It was the aim of this study to measure spectral transmission of the human crystalline lens in situ. Method: The crystalline lens was illuminated by one of four light-emitting diodes of different colors. The relative spectral transmittance of the human crystalline lens was measured with the Purkinje-Sanson mirror images over a wide range of ages. Result: The study evaluated 36 crystalline lenses of 28 subjects aged 21–76 years. There was a significant correlation between the age and spectral transmittance for blue light. Conclusion: Spectral transmittance of the crystalline lens in situ could be measured with Purkinje-Sanson mirror images.

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“…These findings agree with the well-known fact that human lenses become progressively more yellow during the process of aging [9,10,11,12,13,14,15,18]. …”

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Age-Related Changes in Spectral Transmittance of the Human Crystalline Lens in situ

Sakanishi

Awano²,

Mizota³

et al. 2012

Ophthalmologica

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show abstract

“…6) rules out this mechanism and supports conventional absorption. It is known that some absorption, increasing with age, does occur in the human lens (Ranjan and Beedu, 2006). In the pig lens, the fractional absorption at 532 nm is reported to be approximately 0.003 for light traversing its full thickness, indicating that it is quite transparent at this wavelength (Lei and Yao, 2006).…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Photoacoustic Imaging of Ocular Tissues

Silverman

Kong

Chen

et al. 2010

Ultrasound in Medicine & Biology

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Optical coherence tomography (OCT) and ultrasound (US) are methods widely used for diagnostic imaging of the eye. These techniques detect discontinuities in optical refractive index and acoustic impedance respectively. Because these both relate to variations in tissue density or composition, OCT and US images share a qualitatively similar appearance. In photoacoustic imaging (PAI), short light pulses are directed at tissues, pressure is generated due to a rapid energy deposition in the tissue volume, and thermoelastic expansion results in generation of broadband US. PAI thus depicts optical absorption, which is independent of the tissue characteristics imaged by OCT or US. Our aim was to demonstrate the application of PAI in ocular tissues and to do so with lateral resolution comparable to OCT. We developed two PAI assemblies, both of which used single-element US transducers and lasers sharing a common focus. The first assembly had optical and 35-MHz US axes offset by a 30° angle. The second assembly consisted of a 20-MHz ring transducer with a coaxial optics. The laser emitted 5-ns pulses at either 532-nm or 1064-nm, with spot sizes at the focus of 35-μm for the angled probe and 20-μm for the coaxial probe. We compared lateral resolution by scanning 12.5-μm diameter wire targets with pulse/echo US and PAI at each wavelength. We then imaged the anterior segment in whole ex vivo pig eyes and the choroid and ciliary body region in sectioned eyes. PAI data obtained at 1064 nm in the near infrared had higher penetration but reduced signal amplitude compared to that obtained using the 532-nm green wavelength. Images were obtained of the iris, choroid and ciliary processes. The zonules and anterior cornea and lens surfaces were seen at 532 nm. Because the laser spot size was significantly smaller than the US beamwidth at the focus, PAI images had superior resolution than those obtained using conventional US.

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“…21,32,[34][35][36][37][38][39][40][41][42][43] Thus increased lens autofluorescence can be a biomarker indicative of more extensive underlying damage to the proteins due to glycation.…”

Section: Significance Of Lens Autofluorescencementioning

confidence: 99%

Simultaneous Noninvasive Clinical Measurement of Lens Autofluorescence and Rayleigh Scattering Using a Fluorescence Biomicroscope

Burd

Lum

Cahn

et al. 2012

J Diabetes Sci Technol

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Spectroscopic and biochemical correlations during the course of human lens aging

Cited by 14 publications

References 28 publications

Age-Related Changes in Spectral Transmittance of the Human Crystalline Lens in situ

Age-Related Changes in Spectral Transmittance of the Human Crystalline Lens in situ

High-Resolution Photoacoustic Imaging of Ocular Tissues

Simultaneous Noninvasive Clinical Measurement of Lens Autofluorescence and Rayleigh Scattering Using a Fluorescence Biomicroscope

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