Spectroscopic Analysis of Hemolymph from the American Lobster (<i>Homarus americanus</i>)

Bolton, Jason; Collins, S.D.; Smith, Robert L.; Perkins, Brian; Bushway, Rodney J.; Bayer, Robert C.; Vetelino, J.F.

doi:10.2983/035.028.0421

Cited by 13 publications

(10 citation statements)

References 17 publications

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“…Haemolymph protein concentration is considered a means of determining the physical condition or vitality of live lobsters (Stewart and Li 1969;Bolton et al 2009). In accordance with Filiciotto et al (2014), the Representative sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) analysis in 6% polyacrylamide.…”

Section: Discussionmentioning

confidence: 99%

“…Alterations in haemolymph protein concentration have been associated with various environmental stressors (Baden et al 1990;Bjerregaard 1991;Chen et al 1994;Sánchez et al 2001). Haemolymph protein concentration has been considered a means of determining the physical condition or vitality of live lobsters (Stewart and Li 1969;Bolton et al 2009;Celi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Shipping noise affecting immune responses of European spiny lobster (Palinuruselephas)

et al. 2015

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Marine crustaceans are influenced by numerous environmental factors. Environmental stress from pollutants seems to affect their metabolism, growth, moulting, survival, and immune defence. Recently, it has become clear that there is an impact of the global increase in sea noise levels due to shipping traffic on crustacean welfare. Considering the ecological and commercial importance of the European spiny lobster (Palinurus elephas (Fabricius, 1787)) in most parts of the Mediterranean coastal area, in the present study we investigated whether shipping noise pollution contribute to changing the immune parameters of stress in the European spiny lobster. The animals were exposed to a mix of noises produced by different typologies of the boat played back in a tank, and the values of cellular and humeral parameters were evaluated. Total haemocyte count (THC), haemolymphatic protein concentration, phenoloxidase (PO) activity in cell-free haemolymph, and heat shock protein 27 (Hsp27) expression in haemocyte lysate were considered potential biomarkers of stress. THC and PO activity decreased significantly, whereas total protein and Hsp27 expression increased significantly. Overall, the results demonstrate that the stressful conditions investigated in this study affect both cellular and biochemical parameters in the European spiny lobster.Résumé : Les crustacés marins sont sous l'influence de nombreux facteurs environnementaux. Le stress environnemental provenant des polluants semble avoir un impact sur le métabolisme, le développement, la mue, la capacité de survie et les défenses immunitaires. Il a récemment été découvert que l'influence croissante du niveau sonore marin, causé par le trafic maritime, avait un impact non négligeable sur la santé des crustacés. Compte tenu de l'importance économique et écologique de la langouste européenne (Palinurus elephas (Fabricius, 1787)) tout au long de la côte Méditerranéenne, nous nous sommes demandé dans cette étude si la pollution sonore liée au trafic maritime jouait un rôle sur les paramètres du stress immunitaire de la langouste européenne. Pour le vérifier, ces animaux ont été placés dans des aquariums et ont été exposés à un échantillon de bruits produits par différents bateaux. Pour cela, des données sur les paramètres cellulaires et humorales ont été recensés. Les paramètres suivants tels que : le compte total d'haemocyte (THC), la concentration en protéines haemolymphatiques, l'activité de la phenoloxidase (PO) sur les cellules libres de l'haemolymphe, l'expression de la protéine de choc thermique 27 (Hsp27) sur l'haemocyte lysate, ont été considérés comme de potentiels indicateurs de stress. Le THC et l'activité du PO présentent des baisses significatives tandis que le taux de protéines et l'expression du Hsp27 ont augmentés de manières significatives. Dans l'ensemble, les résultats de cette étude ont montré que les paramètres de stress qui ont été testés dans ce cas ont un impact, à la fois sur les paramètres cellulaires et biochimiques de la langouste européenne.Mots-c...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shipping noise affecting immune responses of European spiny lobster (Palinuruselephas)

et al. 2015

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“…Total protein concentration was found to be a strong predictor of shell disease, with low protein concentrations associated with higher odds of developing shell disease (Theriault et al, 2008). Haemolymph protein concentration has been considered as a means of determining physical condition or vitality of live lobsters (Stewart and Li, 1969;Bolton et al, 2009).…”

Section: Haemolymph Protein Concentrationmentioning

confidence: 99%

Health management during handling and live transport of crustaceans: A review

Fotedar

Evans

2011

Journal of Invertebrate Pathology

120

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“…). As the nutritional condition of an animal reflects the extent to which reserves have accumulated to allow normal physiological function and growth (Moore, Smith & Loneragan ), such biochemical parameters can be used to evaluate the fitness of an animal, and to indicate the presence of environmental stressors (Bolton, Collins, Smith, Perkins, Bushway, Bayer & Vetelino ; Hu, Wang, Tsang, Cheung & Shin 2010; Fotedar & Evans ).…”

Section: Introductionmentioning

confidence: 99%

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

et al. 2016

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Five treatments (short‐neck clam Ruditapes philippinarum and four formulated diets with the green microalgae Dunaliella tertiolecta or the golden‐brown microalgae Isochrysis galbana in the ratio (dw/dw) of 5% or 10% to that of clam meat) were used to investigate the growth performance and haemolymph quality of juvenile Chinese horseshoe crabs Tachypleus tridentatus under laboratory culture. At the end of the 12‐week study, various growth and moulting indicators as well as haemolymph biochemical parameters did not show any significant differences among the treatments. Significantly higher percentages of haemolymph amoebocyte viability and granular‐spherical amoebocyte state were noted in both 5% and 10% I. galbana‐supplemented diets compared to that of clam meat. However, the percentages of these two haemolymph parameters in juveniles fed with D. tertiolecta decreased significantly at the end of the experiment. Such differences were attributed to the presence of polyunsaturated fatty acid (PUFA), notably eicosapentaenoic acid (EPA), in I. galbana that was absent in D. tertiolecta and clam meat. Like many marine invertebrates, juvenile horseshoe crabs do not naturally obtain sufficient PUFAs from their diets to meet developmental and physiological needs; hence, supplementing microalgae with high levels of EPA in a protein‐rich diet boosts immune competence and the health status of juvenile horseshoe crabs while under culture.

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Spectroscopic Analysis of Hemolymph from the American Lobster (Homarus americanus)

Cited by 13 publications

References 17 publications

Shipping noise affecting immune responses of European spiny lobster (Palinuruselephas)

Shipping noise affecting immune responses of European spiny lobster (Palinuruselephas)

Health management during handling and live transport of crustaceans: A review

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

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