Spectroscopic analyses on interaction of o-Vanillin-d-Phenylalanine, o-Vanillin-l-Tyrosine and o-Vanillin-l-Levodopa Schiff Bases with bovine serum albumin (BSA)

Gao, Jingqun; Guo, Yuwei; Wang, Jun; Wang, Zhiqiu; Jin, Xudong; Chun-ping, Cheng; Liu, Ying; Li, Kai

doi:10.1016/j.saa.2010.12.077

Cited by 62 publications

(10 citation statements)

References 51 publications

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“…NO exerts inflammatory effects by stimulating COX-2 and iNOS. 29 Excessive production of NO can cause inflammatory diseases by stimulating COX-2 or iNOS. The effect of SAM and SAM + N-GQDs on NO yield was tested in RAW 264.7 cell lines after LPS induction.…”

Section: Resultsmentioning

confidence: 99%

Nitrogen-Doped Graphene Quantum Dot-Combined Sodium 10-Amino-2-methoxyundecanoate: Studies of Proinflammatory Gene Expression and Live Cell Imaging

et al. 2018

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Marine cyanobacteria are renowned for producing bioactive secondary metabolites with great structural diversity via mixed biosynthetic pathways. Lyngbya sp., a marine cyanobacterium, produces many metabolites with anti-inflammatory potentials; nevertheless, its bioactive metabolites exercising providing protection against inflammation has been deciphered inadequate. In this study, the ethanolic fraction of the Lyngbya sp. extract was purified and identified as sodium 10-amino-2-methoxyundecanoate (SAM) using Fourier-transform infrared spectroscopy, nuclear magnetic resonance, and electron spray ionization-mass spectroscopy. SAM showed prominent inhibition of inflammation, which was analyzed by reactive oxygen species generation and nitric oxide (NO) inhibition assay. Furthermore, the anti-inflammatory potentials of SAM were evaluated in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cell lines by fluorescence-activated cell sorting analysis, which evidenced prominent decrease in COX-2 expression (∼90%) with SAM-treated cells than the control. Subsequently, a semiquantitative real-time polymerase chain reaction analysis also revealed the downregulation of COX-2, iNOS, TNF-α, NF-κß, IL-1α, IL-1ß, IL-4, and IL-6 gene expression in SAM-treated LPS-induced RAW 264.7 cells. To further enhance the delivery of SAM into the cells, it was combined with N-doped graphene quantum dots (N-GQDs) for the anti-inflammatory potentials. It resulted in improved downregulation of COX-2, iNOS, TNF-α, NF-κß, IL-1α, IL-1ß, IL-4, and IL-6 than cells treated with SAM alone. Conclusively, N-GQDs combined with SAM have the effective therapeutic potential as an inhibitor of inflammation by modulating the expression of different cytokine genes.

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Section: Resultsmentioning

confidence: 99%

Nitrogen-Doped Graphene Quantum Dot-Combined Sodium 10-Amino-2-methoxyundecanoate: Studies of Proinflammatory Gene Expression and Live Cell Imaging

et al. 2018

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“…BSA exhibited an absorption band at 275 nm and produced a fluorescence band at 337 nm in Tris–HCl buffer solution (pH 7.4). It is known that the absorption and fluorescence peaks of the BSA molecule are associated with the presence of aromatic amino acids such as tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe) in its structure …”

Section: Resultsmentioning

confidence: 99%

Deciphering binding mechanism between bovine serum albumin and new pyrazoline compound K4

Bozkurt

Gül

2019

Luminescence

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The binding mechanism of a new and possible drug candidate pyrazoline derivative compound K4 and bovine serum albumin (BSA) was investigated in buffer solution (pH 7.4) using ultraviolet-visible light absorption and steady-state and synchronous fluorescence techniques. The fluorescence intensity of BSA was quenched in the presence of K4. The quenching process between BSA and K4 was examined at four different temperatures. Decrease of the quenching constants calculated using the Stern-Volmer equation and at increasing temperature suggested that the interaction BSA-K4 was realized through a static quenching mechanism. Synchronous fluorescence measurements suggested that K4 bounded to BSA at the tryptophan region.Fourier transform infrared spectroscopy results showed that there was no significant change in polarity around the tryptophan residue The forces responsible for the BSA-K4 interaction were examined using thermodynamic parameters. In this study, the calculated negative value of ΔG, the negative value of ΔH and the positive value of ΔS pointed to the interaction being through spontaneous and electrostatic interactions that were dominant for our cases. This study provides a very useful in vitro model to researchers by mimicking in vivo conditions to estimate interactions between a possible drug candidate or a drug and body proteins. K E Y W O R D S bovine serum albumin, fluorescence quenching, FRET, pyrazoline 1 | INTRODUCTION Studies on the interaction between proteins and fluorescent probes are very important as they act as models for biochemical applications in cells. [1-3] Serum albumins are proteins that constitute approximately 60% of total plasma proteins. These proteins play an important role in the transport of exogenous and endogenous substances such as fatty acids, metal ions, drugs, and dyes. Bovine serum albumin (BSA) and human serum albumin (HSA) are proteins that are frequently used in biochemical and biophysical studies. BSA is the 76% structural homologue of HSA. BSA is generally preferred as a model protein due to its ease of use, low cost, stability, and unusual ligand binding properties in laboratory experiments. [4] Spectroscopic techniques such as ultraviolet-visible (UV-vis) light absorption, and steady-state and time-resolved fluorescence measurements are often used to investigate the interactions between BSA or HSA and fluores-cence probes due to their ease of use, accuracy, high precision, and rapid response. [5][6][7] Pyrazoline derivatives are compounds with antimicrobial, [8] antinociceptive, [9] anticancer, [10,11] antidepressant, [12] and anti-inflammatory [13] properties. In addition to the pharmacological importance of these compounds, they are used as fluorescent compounds because of their strong fluorescence properties and high quantum yields. To understand the interaction of these bioactive fluorescent compounds with protein molecules in the cell, studies in the laboratory environment with model systems constitute a marked research area. [14,15] Therefore, it is very importa...

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“…Clinicopathologic characteristics, including gender, age, primary tumor location, tumor size, histological classification, Bormann classification, lymph node metastasis and T stage (AJCC 7th edition). The clinicopathological staging (TNM) of the primary tumors was determined according to the International Union Again Cancer,15 histological type according to Lauren’s classification and differentiation grade based on WHO classification.…”

Section: Methodsmentioning

confidence: 99%

<p>Clinicopathological features and Borrmann classification associated with HER2-positive in primary gastric cancer</p>

Dai

Zhang

Jin

2019

CEG

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Purpose Human epidermal growth factor receptor 2 (HER2) assesment is important for patients with advanced gastric cancer (GC) to determine trastuzumab therapy is being considered. A study was performed to evaluate the rate of HER2 positivity in patients with primary gastric cancer and to assess the relationship between HER2-positive and Borrmann classification. Patients and methods Four hundred and sixty-one patients with gastric or gastroesophageal junction cancer were confirmed as having adenocarcinoma between 2005 and 2016. HER2 status was assessed using immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH). Tissues were considered to be HER2-positive when assessment revealed either an IHC score of 3+ or IHC score 2+ accompanied by a positive FISH result. Results The HER2-positive rate was significantly higher in men than in women (19% vs 9%; p =0.006). In our study, HER2-positive gastric tumors with differentiated histology were significantly higher. The proportion of HER2-positive gastric tumors of Borrmann classification III or IV was significantly higher than tumors classified as I or II. Conclusions HER2-positive gastric cancer tends to be associated with male gender, differentiated histology, and Borrmann tumor classification of III or IV.

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Spectroscopic analyses on interaction of o-Vanillin-d-Phenylalanine, o-Vanillin-l-Tyrosine and o-Vanillin-l-Levodopa Schiff Bases with bovine serum albumin (BSA)

Cited by 62 publications

References 51 publications

Nitrogen-Doped Graphene Quantum Dot-Combined Sodium 10-Amino-2-methoxyundecanoate: Studies of Proinflammatory Gene Expression and Live Cell Imaging

Nitrogen-Doped Graphene Quantum Dot-Combined Sodium 10-Amino-2-methoxyundecanoate: Studies of Proinflammatory Gene Expression and Live Cell Imaging

Deciphering binding mechanism between bovine serum albumin and new pyrazoline compound K4

<p>Clinicopathological features and Borrmann classification associated with HER2-positive in primary gastric cancer</p>

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