Spectrophotometric Method for Quantitative Determination of Total Anthocyanins and Quality Characteristics of Roselle (<i>Hibiscus sabdariffa</i>)

Sukwattanasinit, Tasamaporn; Burana-osot, Jankana; Sotanaphun, Uthai

doi:10.1055/s-2007-990262

Cited by 20 publications

(12 citation statements)

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“…The total anthocyanin content (TAC) of purple pak-choi was quantified with a modified pH differential method (AOAC official method 2005.2) [45,46]. The TAC was derived using cyanidin-3-glucoside, which has a molar extinction coefficient of 26,900 L cm -1 mol -1 and a molecular weight of 449.2 g mol -1 .…”

Section: Methodsmentioning

confidence: 99%

Effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pak-choi (Brassica campestris ssp. Chinensis Makino)

Zhu

Zhai

et al. 2017

PLoS ONE

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Anthocyanins are secondary metabolites that contribute to red, blue, and purple colors in plants and are affected by light, but the effects of low light on the physiological responses of purple pak-choi plant leaves are still unclear. In this study, purple pak-choi seedlings were exposed to low light by shading with white gauze and black shading in a phytotron. The responses in terms of photosynthetic properties, carbohydrate metabolism, antioxidant enzyme activity, anthocyanin biosynthetic enzyme activity, and the relative chlorophyll and anthocyanin content of leaves were measured. The results showed that chlorophyll b, intracellular CO2 content, stomatal conductance and antioxidant activities of guaiacol peroxidase, catalase and superoxide dismutase transiently increased in the shade treatments at 5 d. The malondialdehyde content also increased under low light stress, which damages plant cells. With the extension of shading time (at 15 d), the relative chlorophyll a, anthocyanin and soluble protein contents, net photosynthetic rate, transpiration rate, stomata conductance, antioxidant enzyme activities, and activities of four anthocyanin biosynthetic enzymes decreased significantly. Thus, at the early stage of low light treatment, the chlorophyll b content increased to improve photosynthesis. When the low light treatment was extended, antioxidant enzyme activity and the activity of anthocyanin biosynthesis enzymes were inhibited, causing the purple pak-choi seedlings to fade from purple to green. This study provides valuable information for further deciphering genetic mechanisms and improving agronomic traits in purple pak-choi under optimal light requirements.

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Section: Methodsmentioning

confidence: 99%

Effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pak-choi (Brassica campestris ssp. Chinensis Makino)

Zhu

Zhai

et al. 2017

PLoS ONE

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“…UKMR-2 calyces at different maturation stages. The analysis result of the total phenolic and anthocyanin contents was obtained from our previous investigation ( Figure 2) Sukwattanasinit et al 2007). The total phenolics content shows increasing progress throughout the ripening period.…”

Section: Chemical Analysismentioning

confidence: 88%

“…In addition to anthocyanins, Roselle calyces are also rich natural source of other chemical compounds including flavonoids, tannins, steroids, phenols, triterpenoids and alkaloids (Brahma et al 2014;Ijeomah et al 2012;Mungole & Chaturvedi 2011;Obouayeba et al 2014). High-Performance Liquid Chromatography (HPLC) has been widely used in the identification and quantification of anthocyanins in the Roselle extract (Kouakou et al 2015;Lislivia et al 2017;Sukwattanasinit et al 2007). Lislivia et al (2017) and Sukwattanasinit et al (2007) reported that delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside are two predominant anthocyanins that can be found in dried roselle calyces.…”

Section: Introductionmentioning

confidence: 99%

“…High-Performance Liquid Chromatography (HPLC) has been widely used in the identification and quantification of anthocyanins in the Roselle extract (Kouakou et al 2015;Lislivia et al 2017;Sukwattanasinit et al 2007). Lislivia et al (2017) and Sukwattanasinit et al (2007) reported that delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside are two predominant anthocyanins that can be found in dried roselle calyces. It has also been reported that cyanidin-3-glucoside and delphinidin-3-glucoside as minor anthocyanins in H. sabdariffa dried calyces (Kouakou et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Phenolic Constituent in Hibiscus sabdariffa cv. UKMR-2 Calyx at Different Harvesting Times

Ali

Zain²,

Latip³

2019

JSM

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The metabolic changes in the phenolics content of Hibiscus sabdariffa cv. UKMR-2 were investigated at different harvesting times based on the High-Performance Liquid Chromatographic method using a photodiode array detector (HPLC-PDA) with gradient elution. The antioxidant activity was also determined using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (DPPH) assay. The cultivation was executed under a control condition, and the calyces were separately harvested at four maturation stages. UKMR-2 calyces were extracted with water via sonication (50°C, 30 min). HPLC-PDA analysis showed two predominant anthocyanins, namely delphinidin-3-O-sambubioside (2.07-2.47 mg/g DW) and cyanidin-3-O-sambubioside (0.55-1.01 mg/g DW). In addition, ascorbic acid (3.34-9.88 mg/g DW), caffeic acid (0.08-0.09 mg/g DW) and chlorogenic acid (0.50-0.65 mg/g DW) were also detected in all maturity stages. Cyanidin-3-Osambubioside content increased as the calyx became more mature, whereas the delphinidin-3-O-sambubioside, ascorbic acid, and chlorogenic acid content declined towards calyx maturity. The antioxidant activity gradually increased as the calyx ripening progressed. However, the activities did not differ significantly between the stages (p>0.05). The high content of total phenolic, total anthocyanins, and free radical scavenging activity were detected at Stage 4 (28-30 DAA) in UKMR-2, suggesting that this stage is the most appropriate maturity stage for harvesting H. sabdariffa cv. UKMR-2 calyces compared to the other maturity stages.

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“…The spectral and chromatographic analyses showed that the isolated compound was delphinidine-3-glucoside [12]. The total anthocyan content in L. chinensis flowers was 0.14% [13].…”

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confidence: 95%

Chemical study of Lophanthus chinensis

2010

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582.949.2:677.46 and L. M. TankhaevaThe genus Lophanthus Adanson (Lamiaceae) contains 20 species [1]. The aerial part of L. chinensis Benth. (pri yang ku) is used in Tibetan medicine for diseases of the cardiovascular and central nervous systems that are comprised by the name "planetary diseases" [2]. Water-soluble polysaccharides from the aerial part were investigated earlier during a chemical study of L. chinensis [3]. Information on other classes of compounds in L. chinensis has not appeared. The goal of our work was to study the composition of extracted substances from the aerial part of L. chinensis.The aerial part of L. chinensis was collected during flowering in August 2008 near Atsagat (Republic of Buryatia, Russia; sample No. L/L1589-08, located in the Herbarium of the Department of Biologically Active Compounds, IGEB, SB, RAS). The species was verified by Doctor of Pharmaceutical Sciences Professor T. A. Aseeva (IGEB, SB, RAS).Dried and ground raw material (0.6 kg) was extracted with EtOH (70%, 8u, 1:20) at 80°C. The alcohol extract was concentrated to an aqueous residue that was extracted with hexane, CHCl 3 , and EtOAc. This produced four fractions: hexane (10.05 g, 1.68% of air-dried mass); CHCl 3 (17.81 g, 2.97%); EtOAc (8.72 g, 1.45%); and H 2 O (157.65 g, 26.17%). The hexane fraction (8 g) was chromatographed over a SiO 2 column (3.5 u 40 cm) using gradients of hexane:CHCl 3 (100:0o75:25) and CHCl 3 :EtOH (100:0o80:20) to isolate 1 {35 mg, mp 138-140°C, [D] D 20 -50° (c 0.3, CHCl 3 )} and 2 {12 mg, mp 309-310°C, [D] D 20 +79° (c 0.32, CHCl 3 )}, which were identified as E-sitosterol and oleanolic acid, respectively [4]. Subfractions containing carotenoids afforded after HPTLC (petroleum ether:Me 2 CO 7:3) 3 [3 mg; UV spectrum (hexane, O max , nm): 420, 446, 474], 4 [15 mg; UV spectrum (hexane, O max , nm): 424, 449, 476], 5 [27 mg; UV spectrum (hexane, O max , nm): 421, 445, 474], and 6 [2 mg; UV spectrum (EtOH, O max , nm): 425, 450, 475], which were characterized as D-and E-carotenes, lutein, and zeaxanthin [5]. The CHCl 3 fraction (15 g) was dissolved in MeOH (1:10) and left at 4°C for 30 h. An amorphous precipitate separated and was repeatedly recrystallized from CHCl 3 :EtOH to afford 7 [157 mg, mp 290-292°C, [D] D 20 +62° (c 1.0, MeOH)], which was identified as ursolic acid [6]. Next the CHCl 3 fraction was chromatographed over a SiO 2 column (3 u 50 cm) using hexane:CHCl 3 (100:0o75:25) and CHCl 3 :Me 2 CO (100:0o0:100). Subfractions were also rechromatographed using TLC (petroleum ether:Et 2 O:HCOOH 62:31:7) to afford 2 (22 mg), 7 (92 mg), 8 [11 mg, mp 202°C. UV spectrum (MeOH, O max , nm): 228, 255, 295, 343], 9 [13 mg, mp ~350°C. UV spectrum (MeOH, O max , nm): 265, 333; +AlCl 3 278, 300, 368, 381; +AlCl 3 /HCl 278, 300, 343, 380; +NaOMe 275, 325, 393], and 10 [19 mg, mp ~330°C. UV spectrum (MeOH, O max , nm): 252, 266, 290, 350; +AlCl 3 272, 300sh, 326, 420; +AlCl 3 /HCl 260, 275, 300, 355, 385; +NaOMe 266, 329, 400]. According to the results, 8, 9, and 10 were identified as scopoletin ...

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Spectrophotometric Method for Quantitative Determination of Total Anthocyanins and Quality Characteristics of Roselle (Hibiscus sabdariffa)

Cited by 20 publications

References 16 publications

Effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pak-choi (Brassica campestris ssp. Chinensis Makino)

Effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pak-choi (Brassica campestris ssp. Chinensis Makino)

Comparison of Phenolic Constituent in Hibiscus sabdariffa cv. UKMR-2 Calyx at Different Harvesting Times

Chemical study of Lophanthus chinensis

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