Spectrophotometric method for quantitative determination of nonionic, ionic and zwitterionic detergents

Rajakumari, Sona; Srinivasan, Malathi; Rajasekharan, Ram

doi:10.1016/j.jbbm.2006.04.004

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…The concentration of TX‐100 in the purified fractions was determined by the method of Rajakumari et al Each of the sample was added to 50 μL of 50 mM Tris–HCl (pH 7.5) and 5 μL of triolein to give a total volume of 1 mL. The mixture was vortexed for 15 s and the TX‐100 concentration was determined spectrophotometrically at 660 nm immediately using a visible spectrophotometer (Biochrom Libra S12, Chemopharm, Cambridge, England).…”

Section: Methodsmentioning

confidence: 99%

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Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

Jamaluddin

Ariff

Wong

2018

Biotechnology Progress

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Aqueous micellar two‐phase system (AMTPS) is an extractive technique of biomolecule, where it is based on the differential partitioning behavior of biomolecule between a micelle‐rich and a micelle‐poor phase. In this study, an AMTPS composed of a nonionic surfactant, Triton X‐100 (TX‐100) was used for purifying a bacteriocin‐like inhibitory substance (BLIS) derived from Pediococcus acidilactici Kp10. The influences of the surfactant concentration and the effect of additives on the partitioning behavior and activity yield of the BLIS were investigated. The obtained coexistence curves showed that the mixtures of solutions composed of different surfactant concentrations (5–30% w/w) and 50% w/w crude load were able to separate into two phases at temperatures of above 60 °C. The optimum conditions for BLIS partitioning using the TX‐100‐based AMTPS were: TX‐100 concentration of 22.5% w/w, CFCS load of 50% w/w, incubation time of 30 min at 75 °C, and back‐extraction using acetone precipitation. This optimal partitioning resulted in an activity yield of 64.3% and a purification factor of 5.8. Moreover, the addition of several additives, such as sorbitol, KCl, dioctyl sulfosuccinate sodium salt, and Coomassie® Brilliant Blue, demonstrated no improvement in the BLIS separation, except for Amberlite® resin XAD‐4, where the activity yield was improved to 70.3% but the purification factor was reduced to 2.3. Results from this study have demonstrated the potential and applicability of TX‐100‐based AMTPS as a primary recovery method for the BLIS from a complex fermentation broth of P. acidilactici Kp10. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2719, 2019

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Section: Methodsmentioning

confidence: 99%

“…To our knowledge, this is the first report on the use of TX‐100‐based AMTPS for BLIS recovery. TX‐100 was chosen for the system because it is the most commonly used nonionic surfactant in research laboratory and therefore can be easily obtainable, and is relatively the cheapest compared to other nonionic surfactants …”

Section: Introductionmentioning

confidence: 99%

Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

Jamaluddin

Ariff

Wong

2018

Biotechnology Progress

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“…The use of infrared spectroscopic methods combined with chemometric techniques has gained popularity as an alternative approach for quantitative determination of ingredients in detergents [6][7][8][9][10]. In many studies the infrared absorption spectra (IR) of nano alumina at different temperatures have been investigated [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Performance of Detergent Powder Formulations Containing Nano Alumina Using Diffuse Reflectance Infrared Spectroscopy and Pattern Recognition Techniques

Khanmohammadi

Rudmianeh

Garmarudi

et al. 2014

J Surfact & Detergents

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An analytical method has been introduced based on diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) coupled with pattern recognition techniques to determine the efficiency of various detergent powder formulations containing nano alumina. In order to reach this aim, different multivariate classification methods such as principal component analysis, hierarchical cluster analysis and linear discriminate analysis (LDA) were utilized for diffuse reflectance spectra to evaluate the classification approach. The best model was predicted by LDA, with a correct classification rate (%CC) of 93.55 %. Furthermore, sensitivity and specificity for the test set were 0.90 and 0.95, respectively. These results confirm the capability of DRIFTS coupled with chemometric techniques for estimating the performance of detergent powder formulations containing nano alumina.

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A colorimetric assay for determination of residual detergent levels in reconstituted membrane protein preparations

Roosloot¹,

Schoen²

2011

Analytical Biochemistry

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Spectrophotometric method for quantitative determination of nonionic, ionic and zwitterionic detergents

Cited by 5 publications

References 18 publications

Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

Evaluating the Performance of Detergent Powder Formulations Containing Nano Alumina Using Diffuse Reflectance Infrared Spectroscopy and Pattern Recognition Techniques

A colorimetric assay for determination of residual detergent levels in reconstituted membrane protein preparations

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