Spectrophotometric method for polyphenols analysis: Prevalidation and application on Plantago L. species

Grubešić, Renata Jurišić; Vuković, Jadranka; Kremer, Dario; Vladimir‐Knežević, Sanda

doi:10.1016/j.jpba.2005.05.004

Cited by 65 publications

(37 citation statements)

References 21 publications

(23 reference statements)

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“…The results revealed that the content of phenolics, flavonoids and alkaloids in different plant parts of D. malabaricum showed the presence of high phenolics and alkaloid content in root extracts whereas the leaf extracts exhibited the presence of high flavonoid content at both fresh weight and dry weight basis. These results are in agreement with previous works reporting high leaf polyphenol proportions in other plants [34], thus confirming that leaf function serves as defense mechanism against UV damage [31]. Exposure to increased levels of UV radiation causes the leaves to redden and increases the concentrations of total phenols and the main flavonoids [35].…”

Section: Discussionsupporting

confidence: 92%

An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz.

Kolar¹

2014

Turk J Bioch

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Objective: The aim of the study was to examine the efficiency of different extraction methods for the determination of total phenolics, flavonoids and alkaloid contents contributing to antioxidant capacity of Delphinium malabaricum. Methods: The extracts of different plant parts (roots, stems and leaves) of Delphinium malabaricum were prepared in aqueous and various organic solvents and the extracts were evaluated for phenolics, flavonoids and alkaloid contents as the equivalents of gallic acid, rutin, and colchicine; respectively. The antioxidant capacity of the extracts was also assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity and ferric reducing antioxidant power assays in both fresh and dry plant tissues and the difference in fresh and dry extracts on phytochemical constituents and antioxidant activities were compared. Results: The aqueous extracts of roots exhibited the highest total phenolic (4.94 mg gallic acid/g fresh weight, 13.4 mg gallic acid/g dry weight) and total alkaloid content (8.05 mg colchicine/g fresh weight, 20.4 mg colchicine/g dry weight) as compared to stem, leaves and other solvent extracts. Whereas, flavonoid contents were found to be highest in the leaf extracts (5.36 mg rutin/g fresh weight, 7.88 mg rutin/g dry weight). Interestingly the aqueous extracts of all the plant parts exhibited highest yield of phenolic, flavonoids and alkaloids as compare to the other solvents used for the extraction. Antioxidant activity assays exhibited considerable antioxidant potential and showed expected significant positive correlation with the phytochemical compounds. Conclusion: The study specified that aqueous extracts are more effective to extract phenols, flavonoids, alkaloids and antioxidants from Delphinium malabaricum than organic extracts and roots have higher level and the alkaloids were found to be higher comparing to that of phenolics and flavonoids content per gram dry weight of plant tissue. Key Words: Delphinium malabaricum, Phenolics, Flavonoids, Alkaloids, Free radicals, Antioxidant activity Conflict of Interest: Authors have no conflict of interest. ÖZETAmaç: Bu çalışmanın amacı, Delphinium malabaricum'un antioksidan kapasitesine katkıda bulunan total fenol, flavonoid ve alkaloid içeriklerin belirlenmesinde farklı ekstraksiyon metodlarının veriminin incelenmesidir. Metod: Delphinium malabaricum'un bitkisinin değişik bölgelerinden (kökler, gövde ve yapraklar) suda ve farklı organik çözücülerde hazırlanan ekstrelerin, fenol, flavonoid ve alkaloid içerikleri sırasıyla gallik asit, rutin ve kolçisin eşdeğeri olarak değerlendirildi. Ayrıca hem taze hem de kuru bitki ekstrelerinin total antioksidan kapasitesi 1,1-difenil-2-pikrilhidrazil serbest radikal süpürme aktivitesi ve ferrik indirgen antioksidan güç testleri ile değerlendirildi. Taze ve kuru ekstrelerin fitokimyasal bileşenleri ve antioksidan aktiviteleri arasındaki fark karşılaştırıldı. Bulgular: En yüksek total fenol (4.94 mg gallik asit/g taze ağırlık, 13.4 mg gallik asit/g kuru ağırlık) ve total ...

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Section: Discussionsupporting

confidence: 92%

An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz.

Kolar¹

2014

Turk J Bioch

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“…11 Rutin was used to calculate the standard curve (20-120 µg/ml, Y=0.0043x+ 0.03682, R 2 =0.9652) and the results were expressed as µg of rutin equivalents per mg of extract.…”

Section: Estimation Of Total Flavonol Contentmentioning

confidence: 99%

Antioxidant, Antibacterial Activities and Identification of Bioactive Compounds from Terminalia chebula Bark Extracts

Arul

Kathirvel²,

Prakash

et al. 2016

FRA

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“…Spectrophotometric determination of total polyphenols and tannins was performed using Folin-Ciocalteu's phenol reagent (FCR) [21] and comprehensive validation of this method was carried out by authors Jurišić Grubešić et al [29]. Table 1 shows the results of the determinations of total polyphenols (TP), tannins (T), β-sitosterol, and bitterness values in above-ground herbal parts of native and cultivated populations of the following Teucrium L. species: T. arduini L., T. botrys L. T. chamaedrys L., T. flavum L., T. montanum L., T. polium L., and T. scordium L. subsp.…”

Section: Resultsmentioning

confidence: 99%

Analysis of polyphenols, phytosterols, and bitter principles in Teucrium L. species

et al. 2012

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Total polyphenols, tannins, β-sitosterol, and bitterness values were determined in native and cultivated populations of Teucrium L. species from Croatia (T. arduini L., T. botrys L., T. chamaedrys L., T. flavum L., T. montanum L., T. polium L., and T. scordium L. subsp. scordioides Schreb.). Teucrium species have long been present in folk medicine for diverse medicinal uses, but little is known about polyphenols, phytosterols, and bitter principles. Spectrophotometrically determined contents of total polyphenols (TP) and tannins (T) significantly varied among investigated Teucrium species and were somewhat higher in native populations. The highest TP and T contents were measured in native specimens of T. montanum (TP: 13.68%; T: 3.48%). Scanning densitometry was used for measurements of β-sitosterol levels in plant samples ranged from 0.056% (T. montanum) to 0.129% (T. botrys), and it was not significantly different between native and cultivated specimens of the same plant species. Bitterness values were similar for native and cultivated samples of the same plant species; the highest was measured for T. montanum (15659). The present study suggests that Teucrium species growing in Croatia have potential for cultivation and might be a valuable source of natural bioactive compounds.

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Spectrophotometric method for polyphenols analysis: Prevalidation and application on Plantago L. species

Cited by 65 publications

References 21 publications

An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz.

An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz.

Antioxidant, Antibacterial Activities and Identification of Bioactive Compounds from Terminalia chebula Bark Extracts

Analysis of polyphenols, phytosterols, and bitter principles in Teucrium L. species

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