Spectrophotometric Method for Determination of Nitrite and Nitrate Levels in Broccoli and Cauliflower with Different Fertilization Treatment

Abstract

Both broccoli and cauliflower are plants that are widely cultivated and consumed. The vegetable also contains Nitrite and Nitrite, which have a negative impact on human health because of the carcinogenic effect. Levels of Nitrite and Nitrate in vegetable are dependent on several factors, one of them is fertilization. The objective of this research is to determine the levels of Nitrite and Nitrate in broccoli and cauliflower without fertilization and with fertilization (natural and chemical). Samples were planted broccoli and cauliflower with different fertilization treatment. Determination of Nitrite and Nitrate levels were done by the colorimetric method (visible spectrophotometric) by using N-(1-Naphthyl) Ethylenediamine Dihydrochloride and Sulfanilic Acid as the dyes and measured at the maximum absorbance wavelength (540 nm) and on the operating time between 11 minutes to 18 minutes. Nitrite and Nitrate levels in various fertilization treatments of broccoli and cauliflower were different significantly. Nitrite and Nitrate levels in broccoli and cauliflower without fertilization are lower than Nitrite and Nitrate levels in broccoli and cauliflower with fertilization. Nitrite and Nitrate levels in broccoli and cauliflower with natural fertilizer fertilization are lower than Nitrite and Nitrate levels in broccoli and cauliflower with chemical fertilizer fertilization.

Innovative Tools for Nitrogen Fertilization Traceability in Organic Farming Products: A Cauliflower Case Study

“…This result suggests that it is possible to substitute totally or partially mineral fertilizers with organic ones, thus promoting the sustainability of organic production, as found in a different environment with other organic materials on the same crop [47]. The nitrate levels in the cauliflower samples in our study are in line with those previously reported for the Chambord F1 cauliflower cultivar [48] and for cauliflower seedlings [49], showing no effect of the six different fertilization strategies applied on the preferential accumulation of these compounds. Given that cauliflower grows well at 18-25 • and, consequently, takes advantage of days with good sunlight, it is presumable that the low concentration of nitrates found in the cauliflowers coming from the six different fertilization strategies may be attributable, in addition to the cultivation techniques, also to the position and exposure of the field, which favored good radiation and helped the reduction of nitrates.…”

Effects of plant growth-promoting rhizobacteria (PGPR) and different fertilizer combinations on yield and quality properties in cauliflower (Brassica oleracea L. var. botrytis)

“…Studies have been shown that NO3 content of plants edible parts were influenced by fertilization amount and type (Liu et al, 2014). Nerdy et al, (2018) reported that NO3 content of cauliflower elevated with increasing nitrogen ratio that incorporated into the soil.…”

Scrutinizes the Sustainable Role of Halophilic Microbial Strains on Oxygen-Evolving Complex, Specific Energy Fluxes, Energy Flow and Nitrogen Assimilation of Sunflower Cultivars in a Suboptimal Environment

“…Nitrate reductase (NR) and nitrite (NIR) activity was measured as suggested by Srivastava (1975) and Losada and Paneque (1971) based on the nitrate reduction at 540 nm. Total nitrite and nitrate-N content were estimated by acid digestion (Nerdy and Putra, 2018). The superscript defines the significant differences among the treatments.…”