Spectrophotometric Flow Injection Methods for Zinc Determination in Pharmaceutical and Biological Samples

Gaubeur, Ivanise; Ávila-Terra, Lúcia Helena S.; Masini, Jorge C.; Suárez-Iha, Maria Encarnación Vázquez

doi:10.2116/analsci.23.1227

Cited by 13 publications

(12 citation statements)

References 33 publications

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“…It is notable that bone also contains high zinc levels [13–17] along with high citrate, as it exists in prostate cells. Some early studies had recognized that impaired oxidation of citrate was involved in bone citrate production; but the prevailing view [such as 4, 10, 11] was that limited isocitrate dehydrogenase was the cause of decreased citrate oxidation although this was never established.…”

Section: The Implications Of Zinc In Net Citrate Productionmentioning

confidence: 99%

The Important Role of Osteoblasts and Citrate Production in Bone Formation: “Osteoblast Citration” as a New Concept for an Old Relationship

Costello

Franklin²,

Reynolds

et al. 2012

TOBONEJ

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It has been known for about seventy years that bone, in all vertebrates, contains uniquely high citrate levels. However, the role of citrate, its source, its regulation, and its implication in normal bone formation and in bone disorders have remained largely unknown. For the past thirty-five years, the relationship of citrate in bone has been a neglected area of attention and research. It has recently been discovered that citrate is critical for the structure of the apatite nanocrystal, and is required to impart the important properties of bone such as its stability, strength, and resistance to fracture. This brings to focus the need for a renewed interest and research into the relationships of citrate in bone formation. A most fundamental question that must be resolved is “What is the source of citrate in bone?”. This presentation provides a historical review of the early research to the present status of citrate implications in bone. This leads to a new concept of the role of osteoblasts as specialized citrate producing cells that provide the source of citrate in bone formation; i.e. the “osteoblast citration” process. This also brings into focus a new insight into the role of zinc in bone in relation to osteoblast citrate production. The genetic/hormonal/metabolic relationships of “net citrate production” are described. The intent of this presentation is to provide the background for a new perspective of the important implications of osteoblasts and citrate in bone formation; which, hopefully, will stimulate a renewed interest and essential research.

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Section: The Implications Of Zinc In Net Citrate Productionmentioning

confidence: 99%

The Important Role of Osteoblasts and Citrate Production in Bone Formation: “Osteoblast Citration” as a New Concept for an Old Relationship

Costello

Franklin²,

Reynolds

et al. 2012

TOBONEJ

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“…DPKSH was synthesized according to a procedure reported in previous paper. 7 The solutions of DPKSH were prepared by dissolving the reagent in ethanol absolute from Merck and diluted in different ethanol concentrations when needed.…”

Section: Reagents and Solutionsmentioning

confidence: 99%

“…[1][2][3][4] Several strategies are used for separation and preconcentration, such as liquid-liquid, solid-liquid and cloud point extraction. [4][5][6][7] In comparison to other strategies, solid-liquid extraction provides simplicity, lower sample contamination and higher enrichment factors, in addition to simple coupling to flow systems, improved sampling throughput and minimal sample and reagent consumption. [8][9][10][11] Recently a number of sorbents have been used for the in-line preconcentration and determination of metal ions including polymeric resins.…”

Section: Introductionmentioning

confidence: 99%

An anionic resin modified by di-2-pyridyl ketone salicyloylhydrazone as a new solid preconcentration phase for copper determination in ethanol fuel samples

Garcia¹,

Gaubeur²

2011

J. Braz. Chem. Soc.

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A adsorção do DPKSH em resina aniônica foi investigada a 25 ± 1 °C e em pH 12 com base em três modelos cinéticos pseudo-primeira ordem, pseudo-segunda ordem e difusão intra-partícula. Os dados experimentais foram aplicados com sucesso aos modelos das isotermas de Langmuir, Freundlich e Dubinin-Radushkevich (D-R). A resina aniônica modificada com DPKSH foi usada na determinação espectrofotométrica do cobre após pré-concentração em linha. Nas melhores condições, foi observada uma resposta linear entre 20 e 80 mg L , respectivamente, com um nível de confiança de 95%, e um fator de pré-concentração igual a 11. O desvio padrão relativo foi estimado em 2,6% para a concentração de 32 mg L -1 Cu(II) (n = 20). Os resultados obtidos na determinação de cobre em amostras de etanol combustível apresentaram concordância, com um nível de confiança de 99%, aos obtidos por espectrometria de absorção atômica com chama (F AAS).The adsorption of DPKSH onto anionic resin was investigated at 25 ± 1 °C and pH 12 on the basis of three kinetic models including pseudo-first order, pseudo-second order and intraparticle diffusion. Isotherm equations including Langmuir, Freundlich and Dubinin-Radushkevich (D-R) were successfully applied to model the experimental data. An anionic resin loaded with DPKSH was used in a flow system for the in-line concentration of copper prior to spectrophotometric determination. Under optimized conditions, a linear response was observed between 20 and 80 µg L , respectively, at the 95% confidence level with an enrichment factor of 11. The relative standard deviation was estimated to be 2.6% for 32 mg L -1 Cu(II) (n = 20). The results obtained for copper determination in ethanol fuel samples agreed with those achieved by flame atomic absorption spectrometry (F AAS) at the 99% confidence level.

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“…Di-2-pyridyl ketone salicyloylhydrazone (DPKSH) is a complexing agent which has been used in the spectrophotometric determination of metal ions, including Fe(II) and Fe(III) in natural waters, 30 Zn in pharmaceutical and biological materials 31,32 point extraction. The aim of this study was then the development of a sensitive, selective and environmentally friendly spectrometric method of determination of nickel, using DPKSH as a complexing agent and cloud point extraction.…”

Section: 2829mentioning

confidence: 99%

An environmentally friendly analytical procedure for nickel determination by atomic and molecular spectrometry after cloud point extraction in different samples

et al. 2012

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Cloud point extraction (CPE) was employed for separation and preconcentration prior to the determination of nickel by graphite furnace atomic absorption spectrometry (GFAAS), flame atomic absorption spectrometry (FAAS) or UV-Vis spectrophotometry. Di-2-pyridyl ketone salicyloylhydrazone (DPKSH) was used for the first time as a complexing agent in CPE. The nickel complex was extracted from the aqueous phase using the Triton X-114 surfactant. Under optimized conditions, limits of detection obtained with GFAAS, FAAS and UV-Vis spectrophotometry were 0.14, 0.76 and 1.5 mg L À1 , respectively. The extraction was quantitative and the enrichment factor was estimated to be 27. The method was applied to natural waters, hemodialysis concentrates, urine and honey samples. Accuracy was evaluated by analysis of the NIST 1643e Water standard reference material.

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Spectrophotometric Flow Injection Methods for Zinc Determination in Pharmaceutical and Biological Samples

Cited by 13 publications

References 33 publications

The Important Role of Osteoblasts and Citrate Production in Bone Formation: “Osteoblast Citration” as a New Concept for an Old Relationship

The Important Role of Osteoblasts and Citrate Production in Bone Formation: “Osteoblast Citration” as a New Concept for an Old Relationship

An anionic resin modified by di-2-pyridyl ketone salicyloylhydrazone as a new solid preconcentration phase for copper determination in ethanol fuel samples

An environmentally friendly analytical procedure for nickel determination by atomic and molecular spectrometry after cloud point extraction in different samples

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