Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form

Abstract

New simple, sensitive, rapid, reproducible and economical spectrophotometric method have been developed for the determination of amoxicillin trihydrate in pharmaceutical bulk and tablet dosage form using citro phosphate buffer pH 7.2. The system obeys Lambert-Beer's law at 231 nm in the concentration range 2.5-50 μg/m. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were found to be 1.0020 x 104mol-1cm,-10.9996 and 0.03906 μg cm-2respectively. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate

Development and validation of RP-HPLC method for determination of amoxicillin residues and application to NICOMAC coating machine

“…2 AMO is official in British Pharmacopeia (BP), 1 European Pharmacopeia (EP) 3 and United States Pharmacopeia (USP) 4 , they include HPLC method for its determination. It is still a limited number of analytical methods that are reported for the determination of AMO including kinetics degradation, [5][6][7] spectrophotometric, [8][9][10][11][12][13] UHPLC UPLC and mass spectrometry, [14][15][16][17][18][19] thin layer chromatography (TLC), [20][21][22] capillary electrophoresis, [23][24][25][26] high performance liquid chromatography (HPLC), [27][28][29][30][31] in vitro dissolution studies, [32][33][34][35][36] amoxicillin residues in animal tissues using SPE-LC, 37 SPE-cation exchange, 38 in eggs using HPLC-FLD, 39 or HPLC-MS 40 and in commercial meat and milk samples 41 using HPLC-FLD. According to the best of our knowledge there is no validated method for the determination of amoxicillin residues and application to cleaning machine in pharmaceutical industries.…”

Spectrophotometric determination of amoxicillin in tablets using sulphanilic acid

“…Однак якщо концетрація КК перевищує вміст її у ліках вдвічі, то вона негативно впливає на визначення АМ. АМ + СК ν(АМ) : ν(КК) 1 1: 0,25 Знайдений вміст АМ, %, 97,6 ν(АМ) : ν(КК) 2 1: 0,5 Знайдений вміст АМ, %, 81,6 1 Мольні співвідношення АМ та КК у ліках. 2 Максимальні мольні співвідношення АМ та КК.…”

Untitled

“…A 5mL aliquot was withdrawn at different time intervals and filtered using a 0.45μm nylon disc filter; each sample was replaced with 5 mL of fresh dissolution medium. The filtered samples were suitably diluted, if necessary, and assayed by measuring the absorbance at 397 nm for Nimesulide 8,9,12 (Table 1 and Fig 1). All brands have slightly curved round, biconvex shape.…”