Spectrophotometric Determination of Tranexamic Acid in Pharmaceutical Bulk and Dosage Forms

Ansari, Tariq Mahmood; Raza, Asad; Rehman, Atta-Ur

doi:10.2116/analsci.21.1133

Cited by 24 publications

(15 citation statements)

References 10 publications

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“…Ninhydrin is a widely used chromogenic reagent to quantify compounds with primary amine [14,15,16,17,18]. The NHN reacts with the primary amine to produce a colored Ruhemann's purple.…”

Section: Methods Imentioning

confidence: 99%

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Quantitative Determination of Milnacipran by Simple Colorimetric Methods

Mubarakunnisa¹,

Rani²,

S³

et al. 2013

CES

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In the present study, two sensitive, precise and accurate spectrophotometric methods have been developed for the determination of milnacipran in bulk and capsule formulation. The first method is based on the reaction of milnacipran with ninhydrin in N,N'-dimethylformamide medium at 80°C temperature to form a colored Ruhemann's purple, which exhibits absorption maximum at 575nm. The second method is based on extraction of milnacipran into chloroform as ion-pair with bromothymol blue. The yellow colored ion-pair complex exhibits absorption maximum at 410nm. Beer's law is obeyed in the concentration ranges 2.5-37.5μg/mL and 2-12μg/mL of milnacipran for methods I and II, respectively. The effect of experimental variables were investigated and optimized. The validation parameters like linearity, sensitivity, accuracy, precision and robustness were checked by following the ICH guidelines. The proposed methods were applied for the analysis of milnacipran in capsule formulation with good results.

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“…Ninhydrin is a widely used chromogenic reagent to quantify compounds with primary amine [14,15,16,17,18]. The NHN reacts with the primary amine to produce a colored Ruhemann's purple.…”

Section: Methods Imentioning

confidence: 99%

“…The amino derivative thus produced undergoes condensation with another molecule of NHN to give a colored Ruhemann's purple or diketohydrindylindene-diketohydrindamine, which shows absorption maximum at 575nm (Figure 2). The possible reaction mechanism, based on the reported methods [14,15,16,17,18], is given in Figure 3. …”

Section: Methods Imentioning

confidence: 99%

Quantitative Determination of Milnacipran by Simple Colorimetric Methods

Mubarakunnisa¹,

Rani²,

S³

et al. 2013

CES

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“…Maximum wavelength determination was done by making standard solution of tranexamic acid with a serie of concentration between 3-25μg / mL. The solutions were measured using a UV-Vis spectrophotometer at a wavelength range of 200-800 nm, then determined the maximum wavelength (Ansari et al, 2005;Arayne et al, 2008).…”

Section: Derivatization For Colorimetrymentioning

confidence: 99%

The performance of derivate FTIR spectrophotometry method compared to colorimetry for tranexamic acid tablet content determination

Nugrahani¹,

Aulia²

2018

Pharmaciana

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The determination of tranexamic acid levels in tablets was performed using colorimetry method in several studies. Based on its structure, tranexamic acid shows very low UV absorption which impacts to the lack of sensitivity. Then, it requires the addition of a chromophore agent to facilitate and enhance the detection limit. Recently, FTIR reported has been established as a direct content determination for some tablet dosage forms as a specific, simple, and rapid method. In this present research, the method's performance was investigated to quantify tranexamic acid in its tablet dosage form directly. The result was compared to a developed colorimetry using ninhydrin reagent. Correlations between the two methods were analyzed using t-test. The good linearity was shown at a concentration range of 0.5 -1.75% w/w of NH group's spectra. Furthermore, the recovery, intra-and inter-day precision also have been proven to be fulfilled the validation requirement. Futhermore, LOD and LOQ of the method were 0.0531%w/w and 0.1770% w/w respectively. These results were next compared with the counterpart. Afterwards, the t-test statistically proved no significant difference of content determination yielded, between these two methods. In conclusion, FTIR can be used for quantifying the content of tranexamic tablet, than the colorimetry. Moreover, FTIR method also has some advantages such as easier, simpler, faster, cheaper and more sensitive than the counterpart methods. Finally, this method was suggested as a complementary for tranexamic content determination directly of its tablet dosage form.

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“…UV-visible spectrophotometric methods are the instrumental methods of choice commonly used in industrial laboratories because of their simplicity, accuracy, precision and low cost. [6][7][8] In the litera-ture, none of the spectrophotometry procedures have been reported for the determination of zolmitriptan in pharmaceutical formulations. This paper describes for the first time the application of UV-visible spectroscopy for the quantitative analysis of zolmitriptan in pharmaceutical raw and dosage forms.…”

Section: Introductionmentioning

confidence: 99%

A Novel Spectrophotometric Method for the Determination of Zolmitriptan in Pharmaceutical Formulations

Raza

Ansari

Niazi

2007

J Chinese Chemical Soc

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A simple, rapid, cost effective and extraction‐free spectrophotometric method has been developed for the determination of zolmitriptan in pharmaceutical raw and dosage forms. The method is based on the charge‐transfer reaction of zolmitriptan in acetonitrile medium with 0.2% 2,3‐dichloro‐5,6‐dicyano‐1,4‐benzoquinone to form a colored product peaking at 555 nm. Beer's law is obeyed in the concentration range 10‐250 μg mL−1 with molar absorptivity of 1.7 × 103 L mole−1 cm−1. The effects of variables such as reagent concentration, time of reaction, color stability and interferences have been investigated to optimize the procedure. The results have been validated analytically and statistically. The proposed method has been successfully applied for the determination of zolmitriptan in pharmaceutical formulations. Results indicate that the method is accurate, precise and reproducible (relative standard deviation < 2%).

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Spectrophotometric Determination of Tranexamic Acid in Pharmaceutical Bulk and Dosage Forms

Cited by 24 publications

References 10 publications

Quantitative Determination of Milnacipran by Simple Colorimetric Methods

Quantitative Determination of Milnacipran by Simple Colorimetric Methods

The performance of derivate FTIR spectrophotometry method compared to colorimetry for tranexamic acid tablet content determination

A Novel Spectrophotometric Method for the Determination of Zolmitriptan in Pharmaceutical Formulations

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