Spectrophotometric Determination of Saponin in Yucca Extract Used as Food Additive

Uematsu, Yoko; Hirata, Keiko; Saito, Kazuo; Kudo, Ichiro

doi:10.1093/jaoac/83.6.1451

Cited by 81 publications

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“…The flavonoid content was estimated using ferric chloride colorimetric method of Mattila and Kumpulairen (2002). Saponin content was quantitatively estimated by spectrometric determination method of Uematsu et al (2000). Determination of alkaloid content was carried out by the method described by Harborne (1998).…”

Section: Qualitative Phytochemical Analysismentioning

confidence: 99%

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Agatemor¹,

Nwodo²,

Anosike³

2018

Afr. J. Biotechnol.

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Cucumber (Cucumis sativus L.) is very common, cultivated throughout the world and often eaten as a raw vegetable without cooking. In this study, the phytochemical and proximate compositions of cucumber were investigated. Quantitative phytochemical analysis of the homogenate of C. sativus fruit showed that reducing sugars (574.36 ± 3.88 mg/g) was highest amount when compared to other phytochemicals, alkaloids (2.22 ± 0.96 mg/g) and flavonoids (2.14 ± 0.56 mg/g) were moderately present, while cyanogenic glycoside (0.21 ± 0.13 mg/g) was the lowest in quantity. Proximate analysis showed that C. sativus fruit contained the following-fibre (1.02 ± 0.01%), moisture (94.2 ± 0.08%), protein (3.01 ± 0.07%), lipid (0.55 ± 0.13%), carbohydrate (0.28 ± 0.09%) and ash (0.94 ± 0.24%) contents.

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Section: Qualitative Phytochemical Analysismentioning

confidence: 99%

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Agatemor¹,

Nwodo²,

Anosike³

2018

Afr. J. Biotechnol.

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“…Saponins are known to have not only foaming and emulsifying properties (Price et al , ), but also a diverse range of biological and pharmacological effects such as antimicrobial, insecticidal and molluscicidal activities (Sindambiwe et al , ; Attele et al , ; Sparg et al , ), as well as haemolytic properties (Oda et al , ). Saponins have wide applications in beverages and confectionery, as well as in cosmetics (Price et al , ; Petit et al , ; Uematsu et al , ) and pharmaceutical products (Sparg et al , ). Flavonoids have also been reported to have multiple biological effects, including anti‐inflammatory, antiviral, anti‐allergic, antioxidant and free radical‐scavenging abilities (Bors and Saran, ; Middleton and Kandaswami, ; Gil et al , ; Sahu and Gray, ; Duthie et al , ).…”

Section: Introductionmentioning

confidence: 99%

An in vitro Study on the DNA Damaging Effects of Phytochemicals Partially Isolated from an Extract of Glinus lotoides

Demma

El‐Seedi

Engidawork

et al. 2012

Phytotherapy Research

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An extract of Glinus lotoides, a medicinal plant used in Africa and Asia for various therapeutic purposes, was recently shown to cause DNA damage in vitro. To further explore the potential genotoxicity of this plant, fractionation of the crude extract was performed using reverse phase solid-phase extraction and a stepwise gradient elution of methanol in water. Four fractions were collected and subsequently analysed for their DNA damaging effects in mouse lymphoma cells using an alkaline version of the comet assay. To identify potential genotoxic and non-genotoxic principles, each fraction was then subjected to liquid chromatography coupled to mass spectrometry, LC-MS/MS. 1D and 2D nuclear magnetic resonance analyses were used to confirm the identity of some saponins. Although fractions containing a mixture of flavonoids and oleanane-type saponins or oleanane-type saponins alone produced no DNA damage, those containing hopane-type saponins exhibited a pronounced DNA damaging effect without affecting the viability of the cells. To conclude, even if this study presents evidence that hopane-type of saponins are endowed with a DNA damaging ability, further studies are needed before individual saponins can be cited as a culprit for the previously reported genotoxicity of the crude extract of G. lotoides.

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“…The saponin content of the sample was determined by double extraction gravimetric method [18,19]. 5 g of the powdered sample was mixed with 50 ML of 20% aqueous ethanol solution in a flask.…”

Section: Determination Of Saponinmentioning

confidence: 99%

Comparative Studies on the Effect of Boiling and Fermentation on Calabash Gourd Melon (Lagenaria siceraria) Seeds

Ileola

Omodara

Filua

2019

AFSJ

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The research was carried out to collect some useful information on Lagenaria siceraria (Calabash gourd melon) seeds. The dried melon seeds were sorted, cleaned, soaked in water for easy removal and dehulled by abrasion to get the cotyledons, which were divided into three parts. The first part was used directly for analysis, the second part was boiled while the third part was fermented and analysed. Boiled melon had the highest fat and protein content while the raw melon had the highest carbohydrate content, however there was no significant increase in the ash content of the samples. There was a significant reduction in the antinutritional factors of boiled and fermented melon samples compared to the raw melon. Processing led to significant increase in the antioxidant activities and vitamin contents. The study observed that processing led to significant increase in the nutritional composition of the melon seeds and significant reduction in the antinutritional factors.

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Spectrophotometric Determination of Saponin in Yucca Extract Used as Food Additive

Cited by 81 publications

References 0 publications

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

An in vitro Study on the DNA Damaging Effects of Phytochemicals Partially Isolated from an Extract of Glinus lotoides

Comparative Studies on the Effect of Boiling and Fermentation on Calabash Gourd Melon (Lagenaria siceraria) Seeds

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