Spectrophotometric Determination of Reducing Sugar in Wines Employing in-Line Dialysis and a Multicommuted Flow Analysis Approach

Silva, Paulo A.B. da; Souza, Gustavo C. S. de; Paim, Ana Paula S.; Lavorante, André F.

doi:10.4067/s0717-97072018000203994

Cited by 7 publications

(3 citation statements)

References 27 publications

(31 reference statements)

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“…The working solution of uric acid was acquired by diluting the stock solution with a suitable distilled water volume, and then pH was adjusted using 0.1 M hydrochloric acid or 0.1 M sodium hydroxide. A stock solution of NC reagent 1000 mg/L was prepared in 10% (v/v) of absolute ethanol [24]. Working solutions of NC reagent were prepared by dilution in distilled water.…”

Section: Solutions and Reagentsmentioning

confidence: 99%

A New Flow Injection System with Merging-Zone Technique for the Determination of Copper(II) by Neocuproine Reagent in Aqueous Solution

Farhood

Taha

2022

Indones. J. Chem.

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A fast, simple, and high throughput sample merging-zone flow injection design was developed to determine copper(II) in aqueous solution. The procedure is based on the reduction of copper(II) to copper(I) by uric acid followed by a direct reaction with Neocuproine reagent (NC). The orange-yellow complex that forms absorb light at 454 nm. All conditions of the new flow injection unit were investigated. The analytical curve of copper(II) was linear with (r2) value of 0.9978, in the range of 0.4 to 40 mg/L with a detection limit of 0.1 mg/L and a quantification limit of 0.3 mg/L. the molar absorptivity was 1.661 × 105 L/mol cm and the recovery range was between 104.9 and 97%. The homemade acrylic valve was low-cost with zero dead volume and high repeatability (n = 7) with an RSD of 2.31%. The dispersion coefficient values were 1.8,1.62, and 1.31 for the concentrations of 5, 15, and 25 mg/L, respectively. The sample throughput was 69 h–1.

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Section: Solutions and Reagentsmentioning

confidence: 99%

A New Flow Injection System with Merging-Zone Technique for the Determination of Copper(II) by Neocuproine Reagent in Aqueous Solution

Farhood

Taha

2022

Indones. J. Chem.

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“…The Fehling method was carried out by inserting 1 mL of Fehling A and Fehling B solutions into a test tube, then adding 2 mL of the sample and heating it in a boiling water bath for 10 minutes. The presence of reducing sugar indicated by the formation of red brick Cu 2 O deposits [23].…”

Section: Alkimia Jurnal Ilmu Kimia Dan Terapanmentioning

confidence: 99%

Hydrolysis Optimization of Beneng Taro Tubers (Xantoshoma undipes K. Koch) as Bioethanol Raw Material

Visiamah¹,

Simanjuntak²

2021

ALKIMIA

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This study conducted to assess the most appropriate method or hydrolysis of beneng taro tuber to optimize the production of bioethanol to investigate the effect of pHs, times, and temperatures on reducing sugar produced. The concentration of reducing sugar was determined using a UV-Vis spectrophotometer. The result obtained indicated that the optimum amount of reducing sugar (910,875 mg/L ) achieved at pH 10, hydrolysis time of 3 hours, and the temperature of 90 °C. Fermentation of reducing sugar using the powdered bark of raru plant produced 0,18765 % (v/v) of bioethanol and fermentation using Saccharomyces cerevisiae produced 0,2116 % (v/v) of bioethanol.

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“…When infecting, CWDE from Ganoderma is responsible to break plant components such as cellulose and lignin and converted into reducing sugar (Wang, 2020). Other than that, UV/Vis Diffuse reflectance spectroscopy has shown its capability to predict reducing sugar (Silva, 2018) which is potential to detect BSR. Yet, there is no research on the use of DR UV/Vis spectroscopy to determine the presence of BSR.…”

Section: Introductionmentioning

confidence: 99%

Prediction Model of Basal Stem Rot (BSR) Based on Ganoderma Boninense Using UV/Vis Diffuse Reflectance Spectroscopy

Iqbal¹,

Mahmudah²,

Susilo³

2021

Teknotan

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Basal Stem Rot (BSR) disease was considered as the most destructive disease in oil palm tree. Ganoderma boninense. fungi causing BSR on oil palm tree, could release cell wall degrading enzymes (CWDE) which responsible to degrade polysaccharide on oil palm tree cell wall such as cellulose and lignin into reducing sugar. In this research optical measurement using UV/Vis Diffuse Reflectance Spectroscopy (DRS) was utilized to qualify and quantify BSR level based on its reducing sugar. Several stages were conducted to qualify and quantify reducing sugar comprising sample preparation, spectral data acquisition and chemometrics analysis. Health Stem (HS) and Infected Stem (IS) were prepared. The two stem condition were dried and powdered separately then mixed into 5 mixtures from both stems (100% HS, 75% HS + 25% IS + 50% HS + 50% IS, 25% HS +75% IS and 100% IS) and duplicated to produce 10 total sample. Reducing sugar was measured for each sample. Other than that, spectrum acquisition data was conducted using UV/Vis DRS. In the final stage, chemometrics analysis was performed where reducing sugar was set as response and spectral data was set as predictor. The result showed that Principal Component Analysis (PCA) could well classify 4 group of mixtures. While for quantitative analysis, Partial Least Square (PLS) and Support Vector Machine Regresion (SVR) were used to develop prediction model of reducing sugar. PLS showed low performance with the highest R 2 val and R 2 cal accounting for 0.218 and 0.019 and RMSEC and RMSECV were 0.019 and 0.023, respectively. Besides, SVMR using first derivative savitzky-golay (DG1) preprocessing showed high R 2 cal and R 2 val accounting for 0.823 and 0.701 with RMSEC and RMSECV were 0.012 and 0.028, respectively.

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Spectrophotometric Determination of Reducing Sugar in Wines Employing in-Line Dialysis and a Multicommuted Flow Analysis Approach

Cited by 7 publications

References 27 publications

A New Flow Injection System with Merging-Zone Technique for the Determination of Copper(II) by Neocuproine Reagent in Aqueous Solution

A New Flow Injection System with Merging-Zone Technique for the Determination of Copper(II) by Neocuproine Reagent in Aqueous Solution

Hydrolysis Optimization of Beneng Taro Tubers (Xantoshoma undipes K. Koch) as Bioethanol Raw Material

Prediction Model of Basal Stem Rot (BSR) Based on Ganoderma Boninense Using UV/Vis Diffuse Reflectance Spectroscopy

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