Spectrophotometric Determination of Phenylmercuric Acetate

Eldridge, A.; Sweet, Thomas R.

doi:10.1021/ac60116a009

Cited by 4 publications

(5 citation statements)

References 7 publications

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“…Aqueous spore suspensions were either nonirradiated, irradiated with 150,000 rad under FIG. 1. Regressions of log surviving fraction against time at 80 C for aqueous suspensions of B. subtilis spores stored in the presence (2,3) or absence (1, 4) of 0.04% phenylmercuric nitrate in phosphate buffer (pH 7), nonirradiated (1, 2) and irradiated 150,000 rad under air (3,4), 0.04% phenylmercuric nitrate in phosphate buffer (pH 7) being added (1, 4) just before the heat treatment.…”

Section: Materuils and Methodsmentioning

confidence: 99%

“…The 3 combined process should facilitate the ready inactivation of those organisms in the mixed flora of the substances which are particularly sensitive to inactivation by any of the individual . 4 bactericidal agents comprising the process.…”

Section: Figmentioning

confidence: 99%

“…time for aqueous suspensions of B. subtilis spores nionirradiated (1,3) and irradiated with 150,000 rad under air (2, 4), with 0.04% phenylmercuric nitrate in phosphate buffer (pH 7) added just before the heat treatment at 70 C (1, 2) and at 90 C (3,4). radiochemical reactions in the spore initiated a sequence of events, which resulted in either spore inactivation or a predisposition of surviving spores to inactivation during storage and heat treatment with the bactericide.…”

Section: Figmentioning

confidence: 99%

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Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide

Deasy¹,

Küster²,

Timoney³

1971

Applied Microbiology

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Aqueous suspensions of Bacillus subtilis NCTC 8236 spores, surviving gamma irradiation from a cesium-137 source, exhibited an enhanced rate of inactivation compared to nonirradiated spores when heated with 0.04% phenylmercuric nitrate. The enhanced rate of inactivation, observable from survival curves, was noted when spores were irradiated with 150,000 rad under air in either the presence or absence of the bactericide. The magnitude of the enhanced inactivation rate increased as the irradiation dose under air increased from 150,000 to 300,000 rad. The inactivation rates of spores surviving irradiation with 150,000 rad under either oxic or anoxic conditions did not exhibit a simple quantitative relationship. The enhancement effect was observed when the severity of the heat treatment was increased by either reducing the pH from 8 to 6 or raising the temperature from 70 to 90 C.

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Section: Materuils and Methodsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

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Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide

Deasy¹,

Küster²,

Timoney³

1971

Applied Microbiology

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“…Phenylmercuric acetate has been determined by spectrophotometric measurements in perchloric acid solution (161). A general method for the determination of phenylethylamines by spectrophotometric measurements is described by Rontondaro (535).…”

Section: Vitaminsmentioning

confidence: 99%

Pharmaceuticals

Slack¹,

Mader²

1959

Anal. Chem.

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The cathode ray polarography of phenylmercuric nitrate

Porter¹

1968

Journal of Pharmacy and Pharmacology

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XPERIMENTS on the absorption of phenylmercuric nitrate by E rubber teats required a simple, rapid and sensitive assay for concentraticns of phenylmercuric nitrate from 0.5 to 20.0pgIml. Both ultraviolet spectrometry (Eldridge & Sweet, 1956) and chemical methods (British Pharmacopoeia, 1963 ; Department of Pharmaceutical Sciences Report, 1962) are relatively insensitive, the spectrometric method also being subject to interference by extractive from rubber teats. Ordinary polarographic methods have been used for higher concentrations of phenylmercuric nitrate, two cathodic waves being observed at half-wave potentials of about -0-2 V and -0.9 V respectively. These have been observed in a number of supporting electrolytes, including hydrochloric acid (Page & Waller, 1949), Britton-Robinson buffers (Benesch & Benesch, 1951) and a glycerol-triethanolamine mixture with gelatin (Osborne, 1950).The cathode ray polarography of phenylmercuric nitrate (04-20.0 pg/ ml) was therefore investigated in hydrochloric acid (O-~N), lithium chloride ( 0 . 2~) and buffer solutions (Britton-Robinson and Sorensen) in the pH range 1.8 to 12.3. In all cases only one wave of analytical importance was seen, the peak voltage being about -0.3 V. Since good linearity, wave shape and sensitivity were found with both 0-2 N lithium chloride and 0.1 N hydrochloric acid, the latter was chosen as supporting electrolyte. EXPERIMENTAL AND RESULTS Apparatus.A Davis differential cathode ray polarograph was used, and peak voltages were recorded with reference to the mercury pool anode.Operation. Trace impurities in the supporting electrolyte became apparent at high instrument sensitivities. Phenylmercuric nitrate concentrations in the range of 04-2.0 pg/ml were therefore examined by comparing 50 mS derivative operation with the twin cell subtractive mode. This enabled subsequent adoption of the more rapid and convenient 50 mS derivative working for lower concentrations. Direct operation was used in the concentration range 2-0-20.0 pglml. Determinations were made at 25", all solutions being previously flushed with nitrogen until dissolved oxygen no longer affected wave shape or peak height (5 min).Calibration graph. To solutions (5 ml) containing 04-20-0 pg/ml of phenylmercuric nitrate (B.D.H.) in a polarographic cell, 0.6 N hydrochloric acid (1 ml) was added and the solution polarographed (starting potential -0.1 V). A ten point calibration graph was linear (r= 0.997, P>0.999), each point representing the mean of six readings from duplicate determinations on each of three solutions prepared from solid

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Spectrophotometric Determination of Phenylmercuric Acetate

Cited by 4 publications

References 7 publications

Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide

Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide

Pharmaceuticals

The cathode ray polarography of phenylmercuric nitrate

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