Spectrophotometric Determination of Paracetamol using a Newly Synthesized Chromogenic Reagent 4-[(2-amino-1, 3thiazol-4-yl)amino]nitro benzene

Abstract

This study describes the new simple and accurate spectrophotometric method for the determination of paracetamol after the formation of azo dye with a new chromogenic reagent 4 [(2-amino-1,3thiazol-4-yl)amino] nitrobenzene to form an orangecoloured product measured at 425 nm. The molecular structure of the newly synthesized compound was confirmed by several spectroscopic techniques such as UV-visible, FTIR, 1HNMR, and mass spectroscopy. Newly synthesized compound was in vitro screened against several bacterial species. The experimental conditions that affect the reaction were carefully optimized and under the optimized conditions, a linear relationship was obtained in the concentration range of 5-25mg.L −1 of paracetamol. A reaction with a new chromogenic reagent has been occurred at a stoichiometric ratio of 1:1. This method has a limit of detection of 1.334 mg.L -1 and Sandell's sensitivity of 0.0235 mg.cm -1 for new azo dye product.

Spectrophotometric estimation of para-aminophenol via oxidative coupling reaction with 4-chlororesorcinol –Application to paracetamol

“…These methods included HPLC [5][6][7],electrochemical [8,9], flow injection [10], and flurometric [11]. The spectrophotometric methods included various types of reactions such as diazotization of p-aminophenol resulted from hydrolysis of paracetamol then coupled with various coupling agent such as 4 [(2-amino-1, 3thiazol-4-yl) amino] nitrobenzene [12], histidine reagent in alkaline medium [13], 1-naphthol [14], 2, 7-dihydroxy naphthalene in alkaline medium [15], hydroxyl analog of the pharmaceutical naproxen [16], phloroacetophenone in an alkaline medium [17]. The oxidative coupling methods have been used with different reagent such as using N-(1-naphthyl) ethylendiamine dihydrochloride in presence of potassium iodate [18], chlorocresol in the presence of dissolved atmospheric oxygen and sodium carbonate [19] and 2, 5-di-hydroxy benzaldehyde in presence of potassium periodate as oxidizing agent [20], oxidation and bleaching color of dye: oxidation with N-bromosuccinimide and bleaching color of rifampicin [21], with bromosuccinimide and bleaching color of Eriochrom Black-T [22].…”

Development and Validation of a RP-HPLC Method for Simultaneous Quantification of Paracetamol and Phenylephrine Hydrochloride

“…Given its significance, numerous researchers have focused on analyzing this drug in its different forms, including tablets, syrups, and injections. Methods employed for estimating paracetamol concentrations include high-performance liquid chromatography [4][5][6], We observe that numerous researchers have heavily relied on optical spectroscopic methods for the quantitative estimation of various drugs [7], including those currently under investigation; flow injection analysis [8], first derivative spectroscopy [9,10], UV-visible spectroscopy [11,12], and electrochemical techniques [13,14]. Figure 1 illustrates the chemical structure of paracetamol.…”