Spectrophotometric determination of N-acetylcysteine in drug formulations with o-phthalaldehyde and isoleucine

Garcı́a-Alvarez-Coque, M.C.; Medina-Hernández, M.J.; Camañas, R. M. Villanueva; Fernández, Carmen

doi:10.1039/an9891400975

Cited by 35 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, it is being studied and utilized for curing HIV infection, cancer and heart disease. 1 Several analytical methods have been reported for NAC determination in pharmaceutical formulations, including spectrophotometry, [2][3][4][5][6][7] fluorimetry, 8,9 chromatography 10,11 and electrochemistry. 12,13 Some of the methods described above are not simple for direct application in a large-scale routine analysis and require expensive or sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

2011

View full text Add to dashboard Cite

Abstract. A flow-injection spectrophotometric method is developed for determination of N-acetyl-Lcysteine (NAC) in pharmaceutical formulations. The method is based on coupled redox-complexation reaction, the first step of which is the reduction of Fe III by NAC; the second one includes the complexation of Fe . The analytical frequency was 60 h -1 . Usual excipients used as additives in pharmaceuticals do not interfere with the analysis of NAC. The proposed method is simple, rapid, sensitive, accurate and precise (repeatability RSD 0.29 %, n = 10), and can be applied for determination of NAC in pharmaceutical formulations up to nanomole quantity. (doi: 10.5562/cca1753)

show abstract

Section: Introductionmentioning

confidence: 99%

“…5,21 The use of TPTZ as chromogenic reagent has improved selectivity, linearity and sensitivity of measurements. Reaction mechanism of the method is based on the coupled redox-complexation reaction between NAC, Fe III and TPTZ.…”

Section: Introductionmentioning

confidence: 99%

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

2011

View full text Add to dashboard Cite

show abstract

“…1 The European Pharmacopeia recommended iodimetric titration for pharmaceutical formulations analysis. 2 Other reported methods described in the literature comprise: titrimetry, 3,4 spectrophotometry, [5][6][7][8][9] fluorimetry, [10][11][12] chromatography, 13,14 potentiometry, 15 conductometry 16 and voltametry [17][18][19] for the determination of NAC in pure form, in dosage forms and in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Radić¹,

Kukoc-Modun²,

Biocic³

2013

Croat. Chem. Acta

View full text Add to dashboard Cite

“…Intravenous and oral administrations of NAC have been extensively used in the management of paracetamol (acetaminophen) poisoning. 1 Several analytical methods have been developed for NAC determination in pharmaceutical preparations, including spectrophotometric, [2][3][4][5][6] fluorometric, 7,8 chromatographic 9,10 and electrochemical methods. 11,12 However, these methods are not sufficiently sensitive and selective, or some of them require expensive instrumentation and are too expensive for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Based on a Coupled Redox-Complexation Reaction

Kukoc-Modun

Radić

2010

ANAL. SCI.

View full text Add to dashboard Cite

Surprisingly, to the authors' knowledge, there are only two published kinetic spectrophotometric methods for the determination of NAC. 15,16 Also, none of the cited methods for the determination of NAC has used Fe 3+ and 2,4,6-trypyridyl-s-triazine (TPTZ) as a reagent solution. In this report, a novel, simple and sensitive kinetic spectrophotometric method with TPTZ as the chromogenic reagent for the determination of NAC is described and validated. Ease of application, sensitivity, short analysis time, low cost and reliability are its main advantages. The initial rate and fixed-time methods, after their optimization and validation, are adopted for the determination of NAC in its pharmaceutical formulations. Experimental ReagentsAll chemicals were of analytical-reagent grade, and solutions were prepared in Milli-Q deionized water.A stock solution of NAC, 1.0 × 10 -2 mol L -1 , was prepared by dissolving 163.2 mg of NAC (Merck, Darmstadt, Germany) in deionized water up to 100.0 mL in volume. The stock solution was stable for at least 30 days when it was stored in a dark bottle at 4 C. Working solutions of lower concentration were prepared daily by appropriate dilution of the stock solution with deionized water.A stock solution of Fe 3+ , 1.0 × 10 -2 mol L -1 , was prepared by dissolving 270.3 mg of FeCl3·6H2O (Kemika, Zagreb, Croatia) in a portion of deionized water. Concentrated hydrochloric acid (0.5 mL) was added to prevent the hydrolysis of iron, before making up to a volume of 100.0 mL.A stock solution of TPTZ (Merck, Germany), 1.0 × 10 -2 mol L -1 , was prepared by dissolving 312.3 mg in 2.0 mL of HCl (c (HCl) = 6.0 mol L -1 ), and diluted to 100.0 mL with deionized water. A stock solution of TPTZ was stored in a dark bottle at 4 C. A novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on a coupled redox-complexation reaction, the first step of which is the reduction of Fe 3+ by NAC; the second one includes the complexation of Fe 2+ , resulting from the preceding redox reaction, with 2,4,6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)2 2+ complex exhibits an absorption maximum at λ = 593 nm. The initial rate and fixed-time (at 5 min) methods were utilized for constructing calibration graphs. The graphs were linear in concentration ranges from 4.0 × 10 -6 to 1.0 × 10 -4 mol L -1 for the initial rate method and 1.0 × 10 -6 to 1.0 × 10 -4 mol L -1 for the fixed-time method, with detection limits of 1.0 × 10 -6 and 1.7 × 10 -7 mol L -1 , respectively. The proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations.

show abstract

Spectrophotometric determination of N-acetylcysteine in drug formulations with o-phthalaldehyde and isoleucine

Cited by 35 publications

References 0 publications

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Based on a Coupled Redox-Complexation Reaction

Contact Info

Product

Resources

About