Spectrophotometric determination of metronidazole and secnidazole in pharmaceutical preparations

Saffaj, T.; Charrouf, M.; Abourriche, A.; Abboud, Y.; Bennamara, A.; Berrada, Mohamed Saleh

doi:10.1016/j.farmac.2004.06.001

Cited by 35 publications

(21 citation statements)

References 12 publications

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“…Different analytical methods for the determination of secnidazole in preparations and bio-samples were employed. These included spectrophotometric (Abdel et al, 2000;Saffaj et al, 2004Saffaj et al, , 2006, packed column supercritical fluid chromatography (Patel et al, 1998), gas chromatography-electron capture negative ionization mass spectrometry (GC-ECNI-MS; Ho et al, 2005) and high-performance liquid chromatography (HPLC; Abdel et al, 2000;Ravi et al, 1997). However, spectrophotometric and supercritical fluid chromatography were not suitable for the pharmacokinetic study of secnidazole in hman plasma because of poor sensitivity and selectivity; mass spectrometry is too expensive to be adopted in most laboratories in developing countries, and the published HPLC method only had a 500 ng/mL (Ravi et al, 1997) quantification limit, which may not be adequate for the analysis of secnidazole in human plasma.…”

Section: Secnidazolementioning

confidence: 99%

Determination of secnidazole in human plasma by high‐performance liquid chromatography with UV detection and its application to the bioequivalence studies

Sun

Zheng

et al. 2007

Biomedical Chromatography

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A simple, accurate, precise and sensitive HPLC-UV method was developed for the determination of secnidazole in human plasma. Secnidazole and tinidazole (IS) were extracted from 0.2 mL of human plasma by ethyl acetate. Secnidazole was then separated by HPLC on a Diamond C(18) column and quantified by ultraviolet detection at 319 nm. The mobile phase consisted of acetonitrile-aqueous 5 mm sodium acetate (30:70, v/v) containing of 0.1% acetic acid adjusted to pH 4.0, and the flow rate was 1.0 mL/min. The low limit of quantification was 0.1 microg/mL. The method was linear over the concentration range 0.1-25.0 microg/mL (R(2) = 1.000). The recovery of secnidazole from human plasma ranged from 76.5 to 89.1%. Inter- and intra-assay precision ranged from 3.3 to 10.7%. Secnidazole in plasma was stable when stored at ambient temperature for 8 h, at -20 degrees C for 2 weeks and at -20 degrees C for three freeze-thaw cycles. The developed method was successfully applied to the pharmacokinetic and bioequivalence studies between test and reference secnidazole tablets following a single 500 mg oral dosage to 20 healthy volunteers of both genders. Pharmacokinetics parameters T(max), C(max), AUC(0-)t, AUC(0-infinity), T(1/2) were determined of both preparations. The analysis of variance (ANOVA) did not show any significant difference between the two preparations and 90% confidence intervals fell within the acceptable range for bioequivalence. It was concluded that the two secnidazole preparations are bioequivalence and may be used interchangeably.

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Section: Secnidazolementioning

confidence: 99%

Determination of secnidazole in human plasma by high‐performance liquid chromatography with UV detection and its application to the bioequivalence studies

Sun

Zheng

et al. 2007

Biomedical Chromatography

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“…CIP in admixtures with MET and ampicillin has been determined by NMR [18]. MET has been analyzed by spectrophotometry [19][20][21][22] and HPLC [23][24][25][26][27][28]. MET in intravenous admixture with CIP has been determined by first-derivative spectrophotometry [29] and LC [30].…”

Section: Introductionmentioning

confidence: 99%

Reversed-Phase Ion-Pair HPLC and TLC-Densitometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Elkady

Mahrouse

2011

Chromatographia

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Two methods are described for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in binary mixture. The first method was based on the ion-pair liquid chromatographic separation of the two drugs on reversed-phase, lBondapak C 18 column (250 mm 9 4.6 mm, 10 lm) Waters. The mobile phase consisted of monobasic potassium phosphate (50 mM, pH 3, adjusted with phosphoric acid) and acetonitrile (65:35, v/v) containing sodium octane sulphonate (50 mM). Flow rate of 1.2 mL min -1 was applied. Quantitation was achieved with UV detection at 280 nm. Linearity, accuracy and precision were found to be acceptable over the concentration range of 3-180 and 15-180 lg mL -1 for CIP and MET, respectively. The second method was based on the TLC separation of the two drugs followed by densitometric measurements of their bands at 280 nm. The separation was carried out on silica gel 60 F 254 plates, using acetonitrile, ammonia, methanol, methylene chloride and hexane (1.3:1.1:2.0:3.0:1.0, v/v) as developing system. The linear regression analysis data were used for the regression line in the range of 1.5-10 lg band -1 for CIP and MET. The two proposed methods were successfully applied to the determination of both drugs in laboratory prepared mixtures and in commercial tablets. The optimized methods proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparation.

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“…Several * Corresponding author. E-mail: asadbzu@yahoo.com methods have been reported for the determination of metronidazole, including spectrophotometry [8][9][10][11][12][13][14][15]21], polarography [16], titrometry [17] and HPLC [8]. Most of these are expensive and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of metronidazole in pharmaceutical pure and dosage forms using p-benzoquinone

Atta-Ur-Rehman

Ijaz

Raza³

2005

JICS

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A simple and accurate spectrophotometric method for the determination of metronidazole in pure and pharmaceutical dosage forms has been developed. The proposed method is based on the reduction of the nitro group to amino group of the drug. This can be achieved by heating a mixture of an alcoholic solution of metronidazole, zinc powder and dilute hydrochloric acid in a water bath at 90 ± 5 °C for 15 min. The cold and clear filtrate reacts with p-benzoquinone to develop a purple color, which absorbs maximally at 526 nm. The calibration graph is linear over the concentration range of 15-190 μg ml -1 with a molar absorptivity of 1.09 × 10 3 l mol -1 cm -1 . The proposed method is applied to commercially available pharmaceutical dosage forms and the results are statistically compared with those obtained by the reference method.

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Spectrophotometric determination of metronidazole and secnidazole in pharmaceutical preparations

Cited by 35 publications

References 12 publications

Determination of secnidazole in human plasma by high‐performance liquid chromatography with UV detection and its application to the bioequivalence studies

Determination of secnidazole in human plasma by high‐performance liquid chromatography with UV detection and its application to the bioequivalence studies

Reversed-Phase Ion-Pair HPLC and TLC-Densitometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Spectrophotometric determination of metronidazole in pharmaceutical pure and dosage forms using p-benzoquinone

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