Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro

Simpson, Matthew C.; Gozzo, J J

doi:10.1016/0022-1759(78)90232-6

Cited by 39 publications

(17 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…QHS assay was performed using the methods of Simpson and Gozzo [13] with some modifications. In brief, 0.2 ml of 20% sheep red blood cells (SRBC) prepared in normal saline was injected to animals, i.p., 4 days prior to the assay.…”

Section: Quantitative Hemolysis Of Sheep Red Blood Cells (Qhs) Assaymentioning

confidence: 99%

Immunomodulation and antitumor activity by a polysaccharide–protein complex from Lycium barbarum

Gan

Zhang

Yang

et al. 2004

International Immunopharmacology

251

167

View full text Add to dashboard Cite

Section: Quantitative Hemolysis Of Sheep Red Blood Cells (Qhs) Assaymentioning

confidence: 99%

Immunomodulation and antitumor activity by a polysaccharide–protein complex from Lycium barbarum

Gan

Zhang

Yang

et al. 2004

International Immunopharmacology

251

167

View full text Add to dashboard Cite

“…Therefore, it is not too surprising that the DTH response was lessened due to DZ2002's ability to effectively block IL-12 production. The QHS assay quantitates the antibody-producing (especially IgM-producing) capacity of plasma cells responding to SRBC (Simpson and Gozzo, 1978). Suppression of QHS by DZ2002 administration indicates that it also has an effect upon antibody-secreting B cells.…”

Section: Tablementioning

confidence: 99%

Inhibition ofS-Adenosyl-l-homocysteine Hydrolase Induces Immunosuppression

Wu¹,

Fu²,

Zhou³

et al. 2005

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Lymphocytes depend on transmethylation reactions for efficient activation and function. These reactions are primarily catalyzed by S-adenosylmethionine-dependent methyltransferases, which convert S-adenosylmethionine to S-adenosyl-Lhomocysteine. S-adenosyl-L-homocysteine is then hydrolyzed by S-adenosyl-L-homocysteine hydrolase to prevent feedback inhibition of transmethylation reactions. By impeding S-adenosyl-L-homocysteine hydrolase, a build-up of S-adenosyl-L-homocysteine occurs, and most intracellular transmethylation reactions cease. Thus, a nontoxic inhibitor of this enzyme might be a useful immunosuppressive therapeutic agent. We identified a potent reversible type III inhibitor of S-adenosyl-L-homocysteine hydrolase, DZ2002 [methyl 4-(adenin-9-yl)-2-hydroxybutanoate], and determined its cytotoxic and immunologic effects. We demonstrated that DZ2002 blocked S-adenosyl-Lhomocysteine hydrolase more effectively than a type I inhibitor, but cytotoxicity from DZ2002 was greatly reduced. Although DZ2002 did not prevent concanavalin A-induced T cell proliferation or interleukin (IL)-2 production, it significantly reduced both a mixed lymphocyte reaction and IL-12 production from in vitro-stimulated splenocytes. In addition, levels of CD80 and CD86 on human monocytic THP-1 cells were decreased in a dose-dependent manner in the presence of 0.1 to 10 M DZ2002, and decreases were also seen in IL-12 and tumor necrosis factor-␣ production from both mouse thioglycollatestimulated peritoneal macrophages and THP-1 cells. In vivo, DZ2002 significantly suppressed a delayed-type hypersensitivity reaction as well as antibody secretion. We conclude that DZ2002's immunosuppressive effects are likely not solely attributed to T cell inhibition but also to the obstruction of macrophage activation and function through reductions in cytokine output and/or T cell costimulation. These data suggest an important dual role for the S-adenosyl-L-homocysteine hydrolase in both macrophage and T cell function.

show abstract

“…The PFC [13] and QHS [14] assays were performed on the mice. After cervical dislocation in the mice, the spleens were taken out and the blood was dried with filter paper for PFC and QHS assays.…”

Section: Methodsmentioning

confidence: 99%

Immunotoxicity of Copper Alginate Fibers in Guinea Pigs and Mice

Gong

Han

Zhang

et al. 2011

Biol Trace Elem Res

View full text Add to dashboard Cite

The relation between copper alginate fibers and immunotoxicity in animals was studied by dividing guinea pigs and mice into control groups and experimental groups. Varied weights of fibers were subcutaneously embedded in the experimental groups, whereas the control groups were operated on simulatively. Morphology analysis, erythrocyte osmotic fragility (EOF) test, direct plaque-forming cell (PFC) assay, quantitative hemolysis spectrophotometry (QHS) assay, macrophages phagocytosis assay, and pathology analysis were used to examine morphology, microstructure, and immunotoxicity. With increasing doses of copper alginate fibers, the EOF of experimental groups increased in contrast with the control group. Moreover, the antibody level decreased based on the results of the PFC and QHS assays, and macrophages phagocytosis descended in relation to dose. However, the immune functions were weakened without time dependence. According to pathologic photographs, the partial organs were damaged, implying bad histocompatibility. Hence, copper alginate fiber is proved to be a harmful material for medical devices.

show abstract

Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro

Cited by 39 publications

References 4 publications

Immunomodulation and antitumor activity by a polysaccharide–protein complex from Lycium barbarum

Immunomodulation and antitumor activity by a polysaccharide–protein complex from Lycium barbarum

Inhibition ofS-Adenosyl-l-homocysteine Hydrolase Induces Immunosuppression

Immunotoxicity of Copper Alginate Fibers in Guinea Pigs and Mice

Contact Info

Product

Resources

About