Spectrophotometric Determination of Hydroperoxide in Diethyl Ether.

Abstract

With solvent mixtures containing alcohols (Table I), Rt values decrease with increase in the molecular weight of the alcohol added (4, 8). No movement of the ions occurred with pentanolchloroform mixtures.Butanol and 2-propanol aided in the slight movement of cobalt and iron only (Table I).Solvent mixtures 14 and 16 containing ketones gave better movement for the cations.Acetone mixtures gave a reasonable R¡ value for iron(III) only.However, the presence of 2-butanone aided in the migration of all four cations. With this solvent, cobalt and nickel gave greater Rf values than iron and copper, because the nitrates of cobalt and nickel form complexes with 2butanone (8).The presence of hydrochloric acid modified the migration tendencies of the ions in each case (Table I), and helped to give constant and reproducible R/ values. When hydrochloric acid was used, iron had an R¡ value of 1.0 in all cases. The R¡ values decreased in the following sequence when acid-containing solvents were employed: Fe > Cu > Co > Ni. (Chloroformmethanol and chloroform-acetone systems were exceptions.)An improved acidic titanium reagent for determining hydroperoxide is described. The reagent is used to determine hydrogen peroxide, ferf-butyl hydroperoxide, 1 -ethoxyethyl hydroperoxide, and the unknown peroxide resulting from the oxidation of diethyl ether on storage or on exposure to ultraviolet radiation.1 -Ethoxyethyl hydroperoxide has been postulated as a product of the oxidation of diethyl ether in air. Samples of diethyl ether, which had been oxidized in storage or by exposure to ultraviolet radiation, are analyzed by the acidic titanium method, using the improved reagent, and by an iodometric method, and the results are compared. Residues from the evaporation of oxidized ether are analyzed in an attempt to identify the oxidation products. Rscent publications (7, 10) show a renewed interest in the acidic titanium method (8, 9) for hydrogen peroxide and various organic andThe addition of phenol to an organic solvent enhanced the polarity and acidity simultaneously. Hence, Rt values for the cations increased in its presence.

IN SITU HYDROGEN PEROXIDE PRODUCTION MAY ACCOUNT FOR A PORTION OF NUV (300–400 nm) INACTIVATION OF STATIONARY PHASE Escherichia coli

“…Attempts to directly measure the in situ production of H202 by NUV, using the spectrophotometric method of Wolfe (1962), were unsuccessful. Overnight cultures were washed several times by centrifugation, resuspended, irradiated, sonicated, and the extracts measured for the presence of H 2 0 2 .…”

Near‐uv‐induced Breaks in Phage Dna: Sensitization by Hydrogen Peroxide (A Tryptophan Photoproduct)

“…;.W lamps in a Rayonet RPR-202 reactor (Southern New England Ultraviolet Co.) with continuous airbubbling as described previously (McCormick et al, 1976). Hydrogen peroxide (H,O,) concentration in the stock TP was measured (Wolfe, 1962) and found to be 0.024).025% by volume. In terms of molecules of H,O, per initial Trp molecule, the yield was about 2%.…”

The effect of an NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leukocyte bactericidal activity

“…The hydrogen peroxide content of the pellet was determined by spectrophotometric determination of the oxidation of titanium tetrachloride (22).…”