Spectrophotometric determination of dolichol and dolichyl derivatives using the Chugaev color reaction

Steen, L.; Wolf, M.; Lagrou, A.; Hilderson, H. J.; Dessel, G. Van; Dierick, W.

doi:10.1016/0003-2697(84)90433-0

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Standard solutions of 40, 30, 20, 10, and 5% (w/v) sucrose were used as standards. Cholesterol was analyzed in all fractions by the procedure of Hilderson (25) and was found to correlate with an increased absorbance at 600 nm. Therefore, in subsequent experiments, fractions were analyzed by absorbance, and appropriate fractions were pooled and concentrated using a 10-kDa molecular mass cut-off filter (Amicon, Milford, MA) to approximately one-fifth volume.…”

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confidence: 99%

Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1

Musch

Koomoa

Goldstein

2004

Journal of Biological Chemistry

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Upon hypotonic volume expansion, skate erythrocytes lose solutes via a pathway that requires participation of anion exchangers (AEs). Three skate AE isoforms (skAEs) are expressed, and at least skAE1 has been shown to mediate this effect when expressed in oocytes. Under isoosmotic conditions, only a small fraction of skAE1 is expressed on the external plasma membrane. Under these conditions, a portion of skAE1 may be found in non-ionic detergent-insoluble regions. However, the detergent-insoluble material is found intracellularly. Cellular volume expansion by hypoosmotic volume expansion but not volume expansion by isoosmotic medium by permeant solutes (ethylene glycol, diethyl urea, or ammonium chloride) stimulates the appearance of skAE1 in the external plasma membrane, and a significant portion of this is found in detergent-insoluble regions. Upon hypoosmotic volume expansion nearly half of the skAE1 is found as oligomers. SkAE1 in these detergent-insoluble fractions is highly tyrosine phosphorylated. These data suggest that volume expansion by hypoosmotic medium stimulates movement of skAE1 from an intracellular pool contained in detergent-insoluble lipid rafts to the plasma membrane. This skAE1 associates to form oligomers that could be involved in the solute efflux that occurs upon volume expansion.

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confidence: 99%

Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1

Musch

Koomoa

Goldstein

2004

Journal of Biological Chemistry

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“…As a model for the The spectrometric determination of dolichol and phosphorylated derivatives via the Chugaev colour reaction has been described. 531 The role of hydroxymethylglutaryl-CoA reductases in regulating the biosynthesis of dolichols and ubiquinones has been re~iewed. '~' Several papers report experiments which re-examine the stereochemistry of formation of cis-isoprenoid residues in the biosynthesis of long-chain polyprenols.…”

Section: Biosynthesis and Metabolismmentioning

confidence: 99%

Carotenoids and polyterpenoids

Britton¹

1985

Nat. Prod. Rep.

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Conditions for Quantitation of Dolichyl Phosphate, Dolichol, Ubiquinone and Cholesterol by HPLC

Elmberger

Eggens

Dallner

1989

Biomedical Chromatography

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Conditions for the isolation and quantitation of dolichyl phosphate, dolichol, cholesterol, and ubiquinone by reversed phase high performance liquid chromatography were investigated. A simple and fast sample preparation procedure using prepacked mini columns was employed. The UV spectra of the fractions obtained were examined and, in the case of dolichol compounds, the maximum absorbance around 205 nm was shown to be linearly dependent on the number of double bonds present in the isoprenolog. The analytical procedure described shows a very broad range of linearity (five orders of magnitude) and detects single dolichyl phosphate isoprenologs in amounts as small as 0.1 ng. The lowest overall recovery, that for dolichyl phosphate, is 77%. Use of isoprenolog 23 and ergosterol as internal standards reduced the variation in the method to 2.5, 4.0 and 5.5% for cholesterol, dolichyl phosphate and dolichol, respectively. The method described was employed to study the lipid composition of rat organs and biological variations in these compositions.

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Spectrophotometric determination of dolichol and dolichyl derivatives using the Chugaev color reaction

Cited by 10 publications

References 8 publications

Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1

Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1

Carotenoids and polyterpenoids

Conditions for Quantitation of Dolichyl Phosphate, Dolichol, Ubiquinone and Cholesterol by HPLC

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