Spectrophotometric determination of carbamazepine and mosapride citrate in pure and pharmaceutical preparations

Frag, Eman Yossri; Zayed, M.A.; Omar, M. M.; Elashery, Sally E.A.

doi:10.1016/j.arabjc.2011.02.023

Cited by 20 publications

(18 citation statements)

References 17 publications

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“…An advantage of the present study over the previously published spectrophotometric method is that it is more sensitive and time saving. It is also inexpensive and relies on a simple apparatus.…”

Section: Introductionmentioning

confidence: 92%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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A sensitive and simple spectrofluorimetric method has been developed and validated for the determination of the anti-epileptic drug carbamazepine (CBZ) in its dosage forms. The method was based on a nucleophilic substitution reaction of CBZ with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer (pH 9) to form a highly fluorescent derivative that was measured at 530 nm after excitation at 460 nm. Factors affecting the formation of the reaction product were studied and optimized, and the reaction mechanism was postulated. The fluorescence-concentration plot is rectilinear over the range of 0.6-8 µg/mL with limit of detection of 0.06 µg/mL and limit of quantitation of 0.19 µg/mL. The method was applied to the analysis of commercial tablets and the results were in good agreement with those obtained using the reference method. Validation of the analytical procedures was evaluated according to ICH guidelines.

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Section: Introductionmentioning

confidence: 92%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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“…The subsequent fragmentation in MS is determined to large extent by the initial bond rupture of molecular ion [6,[17][18][19][20][21][22][23]. A number of mass spectrometric techniques have utilized helping in rationalized the correct pathways of the molecules, among which are: threshold measurement [29] and metastable abundance ratios [30].…”

Section: Mass Spectral (Ms) Fragmentation and Mocs Of Terazosin Ionizmentioning

confidence: 99%

“…On the other hand, computational quantum chemistry can provide additional information about the atoms and bonds, which can be used successfully in an interpretation of experimental results [20]. Application of computational quantum chemistry in addition to experimental results (MS and TA) gives valuable information about the atoms and bonds which helps in the description and prediction of primary fragmentation site of cleavage and subsequent one [6,[21][22][23].…”

Section: Introductionmentioning

confidence: 97%

“…The aim of the present work is focusing on further application of our previously work on various drugs [6,[21][22][23]. This work includes a correlation between mass spectral fragmentation and TA degradation of the terazosin drug and comparing these experimental data with the theoretical MO calculation to identify the weakest bonds ruptured during both mass and thermal studies.…”

Section: Introductionmentioning

confidence: 97%

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Structure characterization of terazosin drug using mass spectrometry and thermal analyses techniques in comparison with semi-empirical molecular orbital (MO) calculations

Zayed

Fahmey²,

El-Desawy³

et al. 2015

J Therm Anal Calorim

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Terazosin (C 19 H 25 N 5 O 4 , MW = 387) drug is a selective alpha 1 antagonist. It is used for lowering the blood pressure. Also, it used for treatment of symptoms of an enlarged prostate and is therefore a drug of choice for men with hypertension and prostate enlargement. In the present study, mass spectrometry (MS) and thermal analyses (TA) were used to investigate the fragmentation decomposition pathways of terazosin and confirmed by semi-empirical molecular orbital (MO) calculation, using PM3 procedure on the neutral and the positively charged species of the drug. These calculations included, bond length, bond order, partial charge distribution, ionization energy and heats of formation (DH f ). The mass spectra and TA fragmentation pathways were proposed and compared to each other to select the most suitable scheme representing the correct fragmentation pathway of the drug in both techniques. This selection helps understanding of metabolism of the drug in vivo system. Therefore, comparison between MS and TA helps in selection, the proper pathway representing the fragmentation of this drug. This comparison successfully confirmed by MO calculation. Graphical Abstract In the present study, mass spectrometry (MS) and thermal analyses (TA) were used to investigate the fragmentation decomposition pathways of terazosin and confirmed by semi-empirical molecular orbital (MO) calculations. The mass spectra and thermal analyses fragmentation pathways were proposed and compared to each other to select the most suitable scheme representing the correct fragmentation pathway of the drug in both techniques. This selection helps understanding of metabolism of the drug in vivo system. Terazosin hydrochloride (C 19 H 25 N 5 O 4 ) drug (marketed ITRIN), has an IUPAC name; 2-[4-(2-tetrahydrofuranyl) carbonyl]-1 piperazinyl-6,7-dimethoxy-4-quinazolinamine monohydrochloride dihydrate, and its stereo structure is given below

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“…Several analytical methods have been reported for determination of antiepileptic drugs in pharmaceutical and biological samples such as spectrophotometry , spectrofluorimetry , ELISA , voltamperometric techniques , TLC , high‐performance TLC , CE , and MEKC , but the most widely used method for monitoring these drugs is LC . Before analysis by HPLC, a sample preparation step is necessary to extract the trace amount of drugs from the biological medium and to remove the interfering components in the matrix.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction and quantification of lamotrigine, phenobarbital, and phenytoin in human plasma and urine samples using solidified floating organic drop microextraction and high‐performance liquid chromatography

Asadi

Dadfarnia

Shabani

et al. 2015

J of Separation Science

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A novel and simple method based on solidified floating organic drop microextraction followed by high-performance liquid chromatography with ultraviolet detection has been developed for simultaneous preconcentration and determination of phenobarbital, lamotrigine, and phenytoin in human plasma and urine samples. Factors affecting microextraction efficiency such as the type and volume of the extraction solvent, sample pH, extraction time, stirring rate, extraction temperature, ionic strength, and sample volume were optimized. Under the optimum conditions (i.e. extraction solvent, 1-undecanol (40 μL); sample pH, 8.0; temperature, 25°C; stirring rate, 500 rpm; sample volume, 7 mL; potassium chloride concentration, 5% and extraction time, 50 min), the limits of detection for phenobarbital, lamotrigine, and phenytoin were 1.0, 0.1, and 0.3 μg/L, respectively. Also, the calibration curves for phenobarbital, lamotrigine, and phenytoin were linear in the concentration range of 2.0-300.0, 0.3-200.0, and 1.0-200.0 μg/L, respectively. The relative standard deviations for six replicate extractions and determinations of phenobarbital, lamotrigine, and phenytoin at 50 μg/L level were less than 4.6%. The method was successfully applied to determine phenobarbital, lamotrigine, and phenytoin in plasma and urine samples.

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Spectrophotometric determination of carbamazepine and mosapride citrate in pure and pharmaceutical preparations

Cited by 20 publications

References 17 publications

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Structure characterization of terazosin drug using mass spectrometry and thermal analyses techniques in comparison with semi-empirical molecular orbital (MO) calculations

Simultaneous extraction and quantification of lamotrigine, phenobarbital, and phenytoin in human plasma and urine samples using solidified floating organic drop microextraction and high‐performance liquid chromatography

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